Anaritide peptide

Anaritide peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-RSSCFGGRMDRIGAQSGLGCNSFRY-OH

H-Arg-Ser-Ser-Cys-Phe-Gly-Gly-Arg-Met-Asp-Arg-Ile-Gly-Ala-Gln-Ser-Gly-Leu-Gly-Cys-Asn-Ser-Phe-Arg-Tyr-OH

25

95.24%

C₁₁₂H₁₇₅N₃₉O₃₅S₃

2724.01

95896-08-5

16137704

Synthetic

Powder

Anaritide is a 25-amino-acid synthetic form of atrial natriuretic peptide. It has been shown to improve renal function (increase glomerular filtration and urinary output) and renal histopathology in laboratory animals with acute renal dysfunction. It increases the glomerular filtration rate by dilating afferent arterioles while constricting efferent arterioles. It also blocks the tubular reabsorption of sodium and chloride, redistributes renal medullary blood flow, disrupts tubuloglomerular feedback, and reverses endothelin-induced vasoconstriction. Anaritide can be used to study the effects on patients with acute tubular necrosis, particularly in improving dialysis free survival.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Allgren RL, Marbury TC, Rahman SN, Weisberg LS, Fenves AZ, Lafayette RA, Sweet RM, Genter FC, Kurnik BR, Conger JD, Sayegh MH. Anaritide in acute tubular necrosis. Auriculin Anaritide Acute Renal Failure Study Group. N Engl J Med. 1997 Mar 20;336(12):828-34. doi: 10.1056/NEJM199703203361203. PMID: 9062091.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.