Mouse CD13 / ANPEP (His Tag) recombinant protein

Mouse CD13 / ANPEP (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May have a role in angiogenesis (By similarity). Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells.

Aminopeptidase N

P150; LAP-1; CD13; GP150; APN; ANPEP; LAP1; PEPN; AP-N; AP-M

Q3UP74

Anpep; Lap-1; Lap1

Human Cells

A DNA sequence encoding the metalloprotease domain (Lys 66-Ser 966) of mouse ANPEP (NP_032512.2) was expressed with a C-terminal polyhistidine tag.

Mouse

The recombinant mouse ANPEP consists of 909 amino acids and has a calculated molecular mass of 104 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 120-130 kDa protein in SDS-PAGE under reducing conditions.

> 97 % as determined by SDS-PAGE

Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is >800 pmoles/min/μg.