Alyteserin-2a peptide

Alyteserin-2a peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

ILGKLLSTAAGLLSNL

Ile-Leu-Gly-Lys-Leu-Leu-Ser-Thr-Ala-Ala-Gly-Leu-Leu-Ser-Asn-Leu

16

96.5%

Synthetic

Powder

Active against primarily G+ bacteria such as S. aureus (MIC 50 uM). It is helical in 50% TFE. Three years later, several peptide mutants were made to modulate peptide properties. While increase in cationic amino acids increases antimicrobial activity as well as cytotoxicity, incorporation of D-amino acids improved peptide selectivity (Conlon JM et al., 2012). Updated 11/2012 GW. Alyteserin-2a has Antibacterial activity. Alyteserin-2a was found in the European midwife toad, Alytes obstetricans.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Conlon JM, Demandt A, Nielsen PF, Leprince J, Vaudry H, Woodhams DC. The alyteserins: two families of antimicrobial peptides from the skin secretions of the midwife toad Alytes obstetricans (Alytidae). Peptides. 2009;30(6):1069-73.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.