(Ala1)-PAR-4 (1-6) amide (mouse) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

(Ala1)-PAR-4 (1-6) amide (mouse) is a synthetic hexapeptide corresponding to the amino-terminal tethered ligand sequence of the mouse protease-activated receptor-4 (PAR-4). PAR-4 belongs to the family of G protein-coupled protease-activated receptors, which are activated by proteolytic cleavage of their amino-terminal exodomain, exposing a new terminus that acts as a tethered ligand to activate the receptor. The peptide sequence mimics this unmasked ligand, thereby enabling receptor activation independently of protease cleavage.

In the mouse PAR-4 sequence, the first residue of the native tethered ligand is replaced by alanine in this analog, a modification often employed to enhance stability or alter receptor interaction properties. This peptide is commonly used experimentally to selectively activate PAR-4 and to study its signaling functions in various cell types and tissues.

• T.R.Faruqi et al., J. Biol. Chem., 275, 19728 (2000)
• M.D.Hollenberg and M.Saifeddine, Can. J. Physiol. Pharmacol., 79, 439 (2001)
• R.A.Henriksen and V.K.Hanks, Arterioscler. Thromb. Vasc. Biol., 22, 861 (2002)
• Sambrano GR, Huang W, Faruqi T, Mahrus S, Craik C, Coughlin SR. Cathepsin G activates protease-activated receptor-4 in human platelets. J Biol Chem. 2000 Mar 10;275(10):6819-23. doi: 10.1074/jbc.275.10.6819. PMID: 10702240.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.