Ala-6-fenycin peptide

Ala-6-fenycin peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

EKYTEAPEYI

Glu-Lys-Tyr-Thr-Glu-Ala-Pro-Glu-Tyr-Ile

10

96.2%

Synthetic

Powder

BLIS: bacteriocin-like inhibitory substances. An acyl chain (C15 to C17 carbons) is attached to E1, residue 2 is an ornithine (represented as K), and the carboxylic group of C-terminal I10 forms an ester bond with the side chain of Y3. Active against Gram-positive bacteria (B. subtilis, L. acidophilus ATCC 4356, B. linens, C. Wmi NCTC 7547, L. monocytogenes, L. innocua, B. cereus, L. innocua ATCC 33090, L. innocua 1572, L. ivanovii 5 NCTC 11007, L. seeligeri AC 82/4, L. welshimeri AC 73/2, (Motta et al., 2007b), and Gram-negative bacteria (S. Gallinarum, E. carotovora 413, A. hydrophila, P. haemolytica, P. haemolytica, clinical isolate). Also inhibited the replication of viruses such as equine arteritis virus (EAV) and feline herpesvirus type 1 (FHV-1) by over 94%. Ala-6-fenycin was found in Bacillus sp. strain P34.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Scopel e silva D, De castro CC, Da silva e silva F, et al. Antiviral activity of a Bacillus sp. P34 peptide against pathogenic viruses of domestic animals. Braz J Microbiol. 2014;45(3):1089-94.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.