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Product Name
AFAP1L1 antibody
- Documents
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Description
AFAP1L1 Rabbit Polyclonal antibody. Positive WB detected in COS-7 cells. Positive IHC detected in human colon tissue, human cerebellum tissue. Observed molecular weight by Western-blot: 75-86 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Monkey; other species not tested.
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Alternative names
AFAP1 like protein 1 antibody; AFAP1L1 antibody; FLJ36748 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AFAP1L1 recombinant protein (Accession Number: NM_152406). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
COS-7 cells were subjected to SDS PAGE followed by western blot with Catalog No:107903(AFAP1L1 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human cerebellum tissue slide using Catalog No:107903(AFAP1L1 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:107903(AFAP1L1 Antibody) at dilution of 1:50 (under 40x lens)
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Background
Actin Filament-Associated Protein of 110 kDa (AFAP-110/AFAP1) is an adaptor protein with multiple protein binding motifs that is known to function as both an actin binding protein and a cSrc activating protein. A homology search of AFAP1 recently identified AFAP1L1 which has a similar sequence, domain structure and cellular localization. AFAP1L1 is highly expressed in human breast, colon and brain tissue. AFAP1L1 interacts with cortactin and involves the invadosomes formation. AFAP1L1 as a prognostic marker has a role in the progression of spindle cell sarcomas.
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