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Product Name
Recombinant AF9 protein (His tag)
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Description
Recombinant Human AF-9 (MLLT3) YEATS Domain Fragment (aa 1-142) is a high-purity protein designed for chromatin biology and epigenetic research. This fragment fully encompasses the YEATS domain, enabling detailed study of histone recognition and transcriptional regulation mechanisms.
Protein AF-9 (MLLT3) is a crucial chromatin reader component of the super elongation complex (SEC) that enhances RNA polymerase II transcription by suppressing polymerase pausing. It specifically recognizes and binds acylated histone H3, showing preferential affinity for crotonylated histones (H3K9cr and H3K18cr) over acetylated or butyrylated forms. This recognition of crotonylation marks, which characterize active promoters and enhancers, allows AF-9 to recruit the SEC complex to chromatin and facilitate DOT1L-mediated H3K79 methylation. The protein plays a vital role in hematopoietic stem cell maintenance by preserving stemness through binding active gene transcription start sites and sustaining H3K79me2 levels. AF-9
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Protein name
Protein AF-9
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Uniprot ID
P42568
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Gene Name
MLLT3; AF9; YEATS3
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 1-142 aa (P42568) were fused with C-terminal 6His tag.
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Sequence
MASSCAVQVK LELGHRAQVR KKPTVEGFTH DWMVFVRGPE HSNIQHFVEK VVFHLHESFP RPKRVCKDPP YKVEESGYAG FILPIEVYFK NKEEPRKVRF DYDLFLHLEG HPPVNHLRCE KLTFNNPTED FRRKLLKAGG DPLEHHHHHH
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 17.67 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 19 kDa band.
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Purity
>90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Validations
Recombinant AF9 protein (His tag) SDS-PAGE
M: Marker; Lane 1: AF9; Lane 2: BSA
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"