ADT6 peptide

ADT6 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Ac-ADTPPV-NH2

Ac-Ala-Asp-Thr-Pro-Pro-Val-NH2

6

95.6%

C₂₈H₄₅N₇O₁₀

639.69

Synthetic

Powder

ADT peptides containing the Ala-Asp-Thr-Pro-Pro-Val (ADTPPV) sequence were derived from a bulge region in the EC1 domain of E-cadherin. Bocsik et al. showed that the ADT6 peptide (Ac-ADTPPV-NH2) reversibly disrupts the intercellular junctions of primary brain endothelial cell monolayers as measured by trans-endothelial electrical resistance (TEER) values

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Bocsik A., Walter F.R., Gyebrovszki A., Fulop L., Blasig I., Dabrowski S., Otvos F., Toth A., Rakhely G., Veszelka S., et al. Reversible Opening of Intercellular Junctions of Intestinal Epithelial and Brain Endothelial Cells With Tight Junction Modulator Peptides. J. Pharm. Sci. 2016;105:754-765.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.