Human ARF1/ADP-ribosylation factor 1 (His Tag) recombinant protein

Human ARF1/ADP-ribosylation factor 1 (His Tag) recombinant protein

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GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).

ADP-ribosylation factor 1

ADP-RIBOSYLATION FACTOR 1; ARF1; RP23-192P17.1

P84077

ARF1

E. coli

A DNA sequence encoding the human ARF1 (P84077) (Met1-Lys181) was expressed with a polyhistidine tag at the N-terminus.

Human

The recombinant human ARF1 consists of 196 amino acids and predicts a molecular mass of 22.5 KDa. It migrates as an approximately 23 KDa band in SDS-PAGE under reducing conditions.

> 85 % as determined by SDS-PAGE