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Product Name
Human ACOT13/THEM2 (His Tag) recombinant protein
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Description
Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Can also hydrolyze 3-hydroxyphenylacetyl-CoA and 3,4-dihydroxyphenylacetyl-CoA (in vitro). May play a role in controlling adaptive thermogenesis (By similarity).
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Protein name
Acyl-coenzyme A thioesterase 13
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Protein short names
Acyl-CoA thioesterase 13
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Uniprot ID
Q9NPJ3
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Gene Name
ACOT13; THEM2; HT012; PNAS-27
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the human THEM2 (NP_060943.1) (Met1-Asn140) was expressed with a polyhistidine tag at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human THEM2 consists of 158 amino acids and predicts a molecular mass of 17.2 KDa. It migrates as an approximately 18 KDa band in SDS-PAGE under reducing conditions.
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Purity
> 85 % as determined by SDS-PAGE
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Validations
Human THEM2 / ACOT13 Protein (His Tag) SDS-PAGE
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