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Product Name
ACSL3 Polyclonal Antibody
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Description
Polyclonal antibody to ACSL3
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse
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Alternative names
ACSL3 antibody; ACS3 antibody; FACL3 antibody; PRO2194 antibody; long-chain-fatty-acid--CoA ligase 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 42-300 of human ACSL3 (NP_004448.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - ACSL3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ACSL3 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Immunohistochemistry - ACSL3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human mammary cancer using ACSL3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ACSL3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using ACSL3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ACSL3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using ACSL3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ACSL3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using ACSL3 antibody at dilution of 1:100 (40x lens).
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Background
Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis (By similarity). Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates (By similarity). Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"