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Product Name
E. coli acnB recombinant protein
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Description
Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate. Also catalyzes the hydration of 2-methyl-cis-aconitate to yield (2R,3S)-2-methylisocitrate. The apo form of AcnB functions as a RNA-binding regulatory protein. During oxidative stress inactive AcnB apo-enzyme without iron sulfur clusters binds the acnB mRNA 3' UTRs (untranslated regions), stabilizes acnB mRNA and increases AcnB synthesis, thus mediating a post-transcriptional positive autoregulatory switch. AcnB also decreases the stability of the sodA transcript.
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Protein name
Aconitate hydratase B
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Protein short names
ESCHERICHIA COLI; YACI; ACNB; YACJ
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Uniprot ID
P36683
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Gene Name
acnB; yacI; yacJ; b0118; JW0114
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the E.Coli (strain K12) acnB (P36683) (Met 1-Val 865) was expressed and purified.
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Protein Species
E. coli
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Molecular weight
The recombinant E.Coli acnB consisting of 865 amino acids and has a calculated molecular mass of 93.5 kDa. It migrates as an approximately 95 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 97 % as determined by SDS-PAGE
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Validations
Escherichia coli acnB Protein SDS-PAGE
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