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Recombinant ACHB protein (His tag)

Cat.#: 544384

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Product Information

  • Product Name
    Recombinant ACHB protein (His tag)
  • Documents
  • Description

    Recombinant Acetylcholine receptor subunit beta (CHRNB1) fragment (residues 24-244) from Homo sapiens, designed for structural and biochemical studies. This 221-amino acid soluble fragment retains key features for investigating acetylcholine-mediated signaling pathways.

    The full-length Acetylcholine receptor subunit beta (CHRNB1) functions as a ligand-gated ion channel that undergoes extensive conformational changes upon acetylcholine binding, leading to opening of an ion-conducting channel across the plasma membrane (PubMed:27375219). Located in the postsynaptic cell membrane as a multi-pass membrane protein, it plays a critical role in neurotransmission and synaptic signaling. The selected fragment (24-244) represents a stable, soluble unit optimized for experimental applications while maintaining relevance to the native protein's functional architecture.

  • Protein name
    Acetylcholine receptor subunit beta
  • Uniprot ID
    P11230
  • Gene Name
    CHRNB1; ACHRB; CHRNB
  • Source/Expression Host
    E. coli
  • Expression Plasmid/cDNA
    DNA encoding 24-244 aa (P11230) were fused with N-terminal 6His tag.
  • Sequence 
    MSKIKHHHHH HGGSSEAEGR LREKLFSGYD SSVRPAREVG DRVRVSVGLI LAQLISLNEK DEEMSTKVYL DLEWTDYRLS
WDPAEHDGID SLRITAESVW LPDVVLLNNN DGNFDVALDI SVVVSSDGSV RWQPPGIYRS SCSIQVTYFP FDWQNCTMVF
SSYSYDSSEV SLQTGLGPDG QGHQEIHIHE GTFIENGQWE IIHKPSRLIQ PPGDPRGGRE GQRQEVIFYL IIRRK
  • Protein Species
    Human
  • Molecular weight
    Predictes a molecular mass of 26.67 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 29 kDa band. ACHB was found to form oligomers in SDS-PAGE analysis, which is consistent with reports in the literature (doi: 10.1111/j.1742-4658.2006.05363.x).
  • Purity
    >85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
  • Activity
    Not tested.
  • Validations

    Recombinant ACHB protein (His tag) SDS-PAGE

    Recombinant ACHB protein (His tag) SDS-PAGE

    M: Marker; Lane 1: ACHB; Lane 2: BSA

    Western Blot of recombinant ACHB (His tag) with anti-His-tag antibody.

    Western Blot of recombinant ACHB (His tag) with anti-His-tag antibody.

    M: Marker; Lane 1: ACHB; Lane 2: Multi-tag protein

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"