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  • Ac-YVAD-AFC peptide

Ac-YVAD-AFC peptide

Not For Human Use, Lab Use Only.

Cat.#: 319648

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Optional Service: TFA RemovalWhat's this?

Special Price 189.0 USD

Availability: 1-2 weeks
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Product Information

  • Product Name
    Ac-YVAD-AFC peptide
  • Documents

    Batch to batch variation of the purity

  • Sequence Shortening
    Ac-YVAD-AFC
  • Sequence
    Ac-Tyr-Val-Ala-Asp-AFC
  • Length (aa)
    4
  • Peptide Purity (HPLC)
    95.64%
  • Molecular Formula
    C33H36F3N5O10
  • Molecular Weight
    719.66
  • CAS No.
    219137-85-6
  • PubChem CID
    16760708
  • Source
    Synthetic
  • Form
    Powder
  • Description
    Ac-YVAD-AFC is a fluorometric peptide substrate used to measure caspase-1 activity. Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm.
  • Storage Guidelines
    Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides
  • References
    • Furuoka M, Ozaki K, Sadatomi D, Mamiya S, Yonezawa T, Tanimura S, Takeda K. TNF-α Induces Caspase-1 Activation Independently of Simultaneously Induced NLRP3 in 3T3-L1 Cells. J Cell Physiol. 2016 Dec;231(12):2761-7. doi: 10.1002/jcp.25385. Epub 2016 Mar 30. PMID: 26989816.
  • About TFA salt

    Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

    TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

    TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

    In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.

  • Molar Concentration Calculator

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  • Percent Concentration Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.

• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.

• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"