Ac-DEVD-pNA peptide

Ac-DEVD-pNA peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Ac-DEVD-pNA

Ac-Asp-Glu-Val-Asp-pNA

4

95.44%

C₂₆H₃₄N₆O₁₃

638.58

189950-66-1

11527474

Synthetic

Powder

The preferred chromogenic substrate for caspase-7 was Ac-DEVD-pNA. Caspase-3 also efficiently cleaved this peptide. Ac-DEVD-pNA was poorly cleaved by caspase-2. The chromogenic tetrapeptide P-nitroanilides Ac-YEVD-pNA and Ac-LEVD-pNA were preferentially cleaved by caspase-1 and-4, respectively, with higher kcat / Km values than related peptides, including the widely used substrate Ac-YVAD-pNA.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Talanian RV, Quinlan C, Trautz S, Hackett MC, Mankovich JA, Banach D, Ghayur T, Brady KD, Wong WW. Substrate specificities of caspase family proteases. J Biol Chem. 1997 Apr 11;272(15):9677-82. doi: 10.1074/jbc.272.15.9677. PMID: 9092497.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.