Ac-DEVD-AMC peptide

Ac-DEVD-AMC peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Ac-DEVD-AMC

Ac-Asp-Glu-Val-Asp-AMC

4

95.65%

C₃₀H₃₇N₅O₁₃

675.64

169332-61-0

9896164

Synthetic

Powder

The peptide Ac-DEVD-AMC (Acetyl-Asp-Glu-Val-Asp-amidomethylcoumarin) is a fluorogenic substrate that can be cleaved specifically by caspase-3. Caspases play a critical role in mammalian apoptosis, and they all contain an active site cysteine nucleophile and cleave their substrate after an aspartic acid residue. Caspase-3 recognizes substrates with the sequence DEVD, while caspase-1 recognizes the sequence YVAD.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Nobel CS, Kimland M, Nicholson DW, Orrenius S, Slater AF. Disulfiram is a potent inhibitor of proteases of the caspase family. Chem Res Toxicol. 1997 Dec;10(12):1319-24. doi: 10.1021/tx970131m. PMID: 9437520.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.