AC 187 peptide

AC 187 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Ac-VLGKLSQELHKLQTYPRTNTGSNTY-NH2

Ac-Val-Leu-Gly-Lys-Leu-Ser-Gln-Glu-Leu-His-Lys-Leu-Gln-Thr-Tyr-Pro-Arg-Thr-Asn-Thr-Gly-Ser-Asn-Thr-Tyr-NH2

25

95.61%

C₁₂₇H₂₀₅N₃₇O₄₀

2890.19

151804-77-2

Synthetic

Powder

AC 187 is an orally active, amylin receptor antagonist with an IC50 of 0.48 nM that displays 38 and 400 fold selectivity over calcitonin and CGRP receptors respectively. Blockade of amylin with AC 187 increases glucagon concentration, accelerates gastric emptying of liquids, and results in an exaggerated post-challenge glycemia. AC 187 alone increases feeding when infused into the area postrema.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Rushing (2001) Inhibition of central amylin signaling increases food intake and body adiposity in rats. Endocrinolog 142(11) 5035 PMID: 11606473
• Mollet (2004) Infusion of the amylin antagonist AC 187 into the area postrema increases food intake in rats. Physiol Behav. 81(1) 149 PMID: 15059694
• Gedulin et al (2006) Role of endogenous amylin in glucagon secretion and gastric emptying in rats demonstrated with the selective antagonist, AC187. Regul.Pept. 137 121 PMID: 16914214

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.