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Product Name
Human ABHD4 (His Tag) recombinant protein
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Description
Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine (By similarity).
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Protein name
Abhydrolase domain-containing protein 4
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Protein short names
AI429574; FLJ12816; ABHD4; ABH4; 1110035H23RIK
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Uniprot ID
Q8TB40
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Gene Name
ABHD4
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the full length of human ABHD4 (NP_071343.2) (Met 1-Asp 342) was expressed, with a polyhistidine tag at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human ABHD4 consists of 360 amino acids and predicts a molecular mass of 41 kDa. It migrates as an approximately 40 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 82 % as determined by SDS-PAGE
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Validations
Human ABHD4 Protein (His Tag) SDS-PAGE
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