3BP-3940 peptide

3BP-3940 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

nBu-CAyl-[Cys(tMeBn(DOTA-AET)-PPTQFC]-OH

nBu-CAyl-[Cys(tMeBn(DOTA-AET)-Pro-Pro-Thr-GIn-Phe-Cys]-OH

7

95.45%

C₆₆H₉₈N₁₄O₁₈S₃

1471.76

2803421-14-7

168830803

Synthetic

Powder

3BP-3940 is a highly potent peptide developed for targeting fibroblast activation protein (FAP), which is commonly expressed in the tumor microenvironment of various solid cancers. The peptide is designed for use in theranostics, combining diagnostic and therapeutic applications. It can be labeled with radionuclides like Gallium-68 (Ga-68) for imaging purposes and Lutetium-177 (Lu-177) for therapy. 3BP-3940 has been optimized for prolonged tumor retention and low uptake in healthy tissues, addressing a major challenge with other FAP-targeting compounds that show rapid clearance from tumors. The development of 3BP-3940 includes automated manufacturing processes and radiolabeling methods, ensuring high purity and stability for clinical use. Initial studies have demonstrated promising results in terms of diagnostic imaging and therapeutic effectiveness in cancer patients.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Greifenstein L, Gunkel A, Hoehne A, Osterkamp F, Smerling C, Landvogt C, Mueller C, Baum RP. 3BP-3940, a highly potent FAP-targeting peptide for theranostics - production, validation and first in human experience with Ga-68 and Lu-177. iScience. 2023 Nov 23;26(12):108541. doi: 10.1016/j.isci.2023.108541. PMID: 38089587; PMCID: PMC10711456.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.