18-CSP peptide

18-CSP peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-SGSLSTFFRLFNRSFTQA-OH

H-Ser-Gly-Ser-Leu-Ser-Thr-Phe-Phe-Arg-Leu-Phe-Asn-Arg-Ser-Phe-Thr-Gln-Ala-OH

18

95.55%

C₉₄H₁₄₀N₂₆O₂₇

2066.26

Synthetic

Powder

18-CSP is an 18 amino acid competence stimulating peptide. 18-CSP is derived from the ComC precursor, processed to a final length of 18 residues and exported to the extracellular medium. S. mutans detects CSP through the ComDE two-component signal transduction system (TCS), which directly activates multiple genes involved in bacteriocin biogenesis, secretion and immunity.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Son M, Kaspar J, Ahn SJ, Burne RA, Hagen SJ. Threshold regulation and stochasticity from the MecA/ClpCP proteolytic system in Streptococcus mutans competence. Mol Microbiol. 2018 Dec;110(6):914-930. doi: 10.1111/mmi.13992. Epub 2018 Nov 11. PMID: 29873131; PMCID: PMC6281771.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.