10Panx peptide

10Panx peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-WRQAAFVDSY-OH

H-Trp-Arg-Gln-Ala-Ala-Phe-Val-Asp-Ser-Tyr-OH

10

95.03%

C₅₈H₇₉N₁₅O₁₆

1242.33

Synthetic

Powder

10Panx mimics a sequence (WRQAAFVDSY) in the second extracellular loop of Panx1 and is a panx-1 mimetic inhibitory peptide that blocks pannexin 1 gap junctions. 10Panx can inhibit Panx1 hemichannel mediated ATP release and inflammasome activation in astrocytes and macrophages and in vivo, pharmacological blockade of Panx1 hemichannels with 10Panx peptide attenuates the progression of acetaminophen induced hepatotoxicity, neuropathic pain and epilepsy.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Wang et al (2007) Modulation of membrane channel currents by gap junction protein mimetic peptides: size matters. Am.J.Physiol.Cell Physiol. 293 C1112 PMID: 17652431
• Bravo et al (2014) Pannexin 1: a novel participant in neuropathic pain signaling in the rat spinal cord. Pain 155(10) 2108 PMID: 25102401
• Chen (2019) Enhanced Macrophage Pannexin 1 Expression and Hemichannel Activation Exacerbates Lethal Experimental Sepsis. Sci Rep. 9(1) 160 PMID: 30655582

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.