(NANP)3 peptide

(NANP)3 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-NANPNANPNANP-OH

H-Asn-Ala-Asn-Pro-Asn-Ala-Asn-Pro-Asn-Ala-Asn-Pro-OH

12

95.12%

C₄₈H₇₄N₁₈O₁₉

1207.2

Synthetic

Powder

(NANP)3 polypeptide is a crucial peptide sequence in the field of malaria research. It is a tandem repeat peptide with the structure NANPNANPNANP, derived from the circumsporozoite (CS) protein of Plasmodium falciparum. This polypeptide serves as a significant protective epitope. Antibodies against (NANP)3 play a vital role in inhibiting the motility of sporozoites, thereby preventing the invasion of host hepatocytes and subsequent blood-stage infection. It has been widely utilized in the development of various malaria vaccine candidates, including synthetic peptides, multiple antigenic peptides (MAPs), polyoxime branched peptides, and virus-like particles (VLPs). The immunogenicity of (NANP)3 makes it a key target for inducing immune responses against malaria, holding great potential in the fight against this deadly disease.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Rudra JS, Mishra S, Chong AS, Mitchell RA, Nardin EH, Nussenzweig V, Collier JH. Self-assembled peptide nanofibers raising durable antibody responses against a malaria epitope. Biomaterials. 2012 Sep;33(27):6476-84. doi: 10.1016/j.biomaterials.2012.05.041. Epub 2012 Jun 12. PMID: 22695068; PMCID: PMC3392361.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.