pTM30 vector (V002607)

LOCUS       40924_43603        4360 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTM30, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Morrison TB, Parkinson JS.
  TITLE     Liberation of an interaction domain from the phosphotransfer region 
            of CheA, a signaling kinase of Escherichia coli
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 91 (12), 5485-5489 (1994)
  PUBMED    8202513
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Morrison TB.
  TITLE     pTM30 expression vector for randomly coding DNA fragments
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4360)
  AUTHORS   Morrison TB, Parkinson JS.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-1997) Pathology, University of Utah, School of 
            Medicine 5B210, Salt Lake City, UT 84132, USA
REFERENCE   4  (bases 1 to 4360)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4360)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "1994"; volume: "91"; issue: "12"; pages: 
            "5485-5489"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (09-SEP-1997) Pathology, University of Utah, School of Medicine 
            5B210, Salt Lake City, UT 84132, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..30
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    38..54
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      60..70
                     /gene="cheY"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /regulatory_class="ribosome_binding_site"
     gene            60..70
                     /gene="cheY"
                     /label=cheY
     misc_feature    74..102
                     /label=Region: cloning site
                     /note="Region: cloning site"
                     /experiment="experimental evidence, no additional details
                     recorded"
     regulatory      103..113
                     /note="ochre translation terminators in all reading frames"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /regulatory_class="terminator"
     rep_origin      187..642
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        850..927
                     /label=lacI promoter
     CDS             928..2007
                     /label=lacI
                     /note="lac repressor"
     protein_bind    2023..2044
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2170..2274
                     /label=AmpR promoter
     CDS             2275..3132
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3306..3894
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4080..4220)
                     /label=bom
                     /note="basis of mobility region from pBR322"