LOCUS       40924_43603        4360 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTM30, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Morrison TB, Parkinson JS.
  TITLE     Liberation of an interaction domain from the phosphotransfer region 
            of CheA, a signaling kinase of Escherichia coli
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 91 (12), 5485-5489 (1994)
  PUBMED    8202513
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Morrison TB.
  TITLE     pTM30 expression vector for randomly coding DNA fragments
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4360)
  AUTHORS   Morrison TB, Parkinson JS.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-1997) Pathology, University of Utah, School of 
            Medicine 5B210, Salt Lake City, UT 84132, USA
REFERENCE   4  (bases 1 to 4360)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4360)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "1994"; volume: "91"; issue: "12"; pages: 
            "5485-5489"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (09-SEP-1997) Pathology, University of Utah, School of Medicine 
            5B210, Salt Lake City, UT 84132, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..30
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    38..54
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      60..70
                     /gene="cheY"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /regulatory_class="ribosome_binding_site"
     gene            60..70
                     /gene="cheY"
                     /label=cheY
     misc_feature    74..102
                     /label=Region: cloning site
                     /note="Region: cloning site"
                     /experiment="experimental evidence, no additional details
                     recorded"
     regulatory      103..113
                     /note="ochre translation terminators in all reading frames"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /regulatory_class="terminator"
     rep_origin      187..642
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        850..927
                     /label=lacI promoter
     CDS             928..2007
                     /label=lacI
                     /note="lac repressor"
     protein_bind    2023..2044
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2170..2274
                     /label=AmpR promoter
     CDS             2275..3132
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3306..3894
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4080..4220)
                     /label=bom
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 gttgacaatt aatcatcggc tcgtataatg tatttaattg tgagcggata acaatttaaa
       61 ggagtgtgaa atgctgcagg atccgatatc aagcttggta cctaactaac taaggcgcct
      121 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatcg tcaaagcaac
      181 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
      241 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      301 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      361 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
      421 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      481 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg
      541 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      601 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttggtgcac tctcagtaca
      661 atctgctctg atgccgcata gttaactgcg ccccgacacc cgccaacacc cgctgacgcg
      721 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
      781 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gcttgaagac
      841 gaaagggtcg acaccatcga atggcgcaaa acctttcgcg gtatggcatg atagcgcccg
      901 gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag
      961 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
     1021 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
     1081 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
     1141 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
     1201 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
     1261 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
     1321 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
     1381 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
     1441 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
     1501 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
     1561 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
     1621 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
     1681 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcgggtat ctcggtagtg
     1741 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgtcaaccac catcaaacag
     1801 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
     1861 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
     1921 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     1981 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     2041 tcattaggca ccccaggctt tacactttat gcttcgactc tagagctcgt gatacgccta
     2101 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     2161 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     2221 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     2281 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     2341 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     2401 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     2461 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     2521 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     2581 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2641 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2701 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     2761 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctcca
     2821 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     2881 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     2941 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3001 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     3061 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     3121 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     3181 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     3241 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3301 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3361 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3421 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     3481 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3541 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3601 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3661 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3721 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3781 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3841 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3901 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3961 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     4021 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     4081 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     4141 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
     4201 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     4261 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     4321 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
//
