Basic Vector Information
For L. plantarum, 10 μg/ml chloramphenicol and 10 μg/ml or (for replica plating) 30 μg/ml erythromycin were used. For E. coli, 10 μg/ml chloramphenicol and 250 μg/ml erythromycin were used.
- Vector Name:
- pNZ5319
- Antibiotic Resistance:
- Erythromycin
- Length:
- 3737 bp
- Type:
- Mutagenesis vector
- Replication origin:
- p15A ori
- Source/Author:
- Lambert JM, Bongers RS, Kleerebezem M.
- Copy Number:
- Medium copy number
- Growth Strain(s):
- DH5alpha
- Growth Temperature:
- 37℃
pNZ5319 vector Vector Map
References
- Lambert JM, Bongers RS, Kleerebezem M. Cre-lox-based system for multiple gene deletions and selectable-marker removal in Lactobacillus plantarum. Appl Environ Microbiol. 2007 Feb;73(4):1126-35. doi: 10.1128/AEM.01473-06. Epub 2006 Dec 1. PMID: 17142375; PMCID: PMC1828656.
pNZ5319 vector Sequence
LOCUS 40924_33702 3737 bp DNA circular SYN 18-DEC-2018
DEFINITION Mutagenesis vector pNZ5319, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3737)
AUTHORS Lambert JM, Bongers RS, Kleerebezem M.
TITLE Cre-lox-based system for multiple gene deletions and
selectable-marker removal in Lactobacillus plantarum
JOURNAL Appl. Environ. Microbiol. 73 (4), 1126-1135 (2007)
PUBMED 17142375
REFERENCE 2 (bases 1 to 3737)
AUTHORS Lambert JM, Bongers RS, Kleerebezem M.
TITLE Direct Submission
JOURNAL Submitted (27-JUN-2005) Health and Safety Department, NIZO Food
Research, Kernhemseweg 2, Ede, Gld 6718ZB, The Netherlands
REFERENCE 3 (bases 1 to 3737)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3737)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
Environ. Microbiol."; date: "2007"; volume: "73"; issue: "4"; pages:
"1126-1135"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(27-JUN-2005) Health and Safety Department, NIZO Food Research,
Kernhemseweg 2, Ede, Gld 6718ZB, The Netherlands"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3737
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 1..648
/gene="cat"
/label=cat
/note="Chloramphenicol acetyltransferase from
Staphylococcus aureus. Accession#: P00485"
protein_bind 772..805
/label=lox71
/note="Left element (LE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
regulatory complement(949..1050)
/label=Tlas
/note="Tlas"
/regulatory_class="terminator"
CDS complement(1165..1899)
/gene="ermBP"
/label=ermBP
/note="rRNA adenine N-6-methyltransferase from Enterococcus
faecalis. Accession#: P0A4D5"
rep_origin 2328..2873
/direction=RIGHT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
regulatory 3285..3311
/label=TpepN
/note="TpepN"
/regulatory_class="terminator"
protein_bind 3505..3538
/label=lox66
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
regulatory 3609..3721
/label=P32
/note="P32"
/regulatory_class="promoter"
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