LOCUS       40924_33702        3737 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mutagenesis vector pNZ5319, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Lambert JM, Bongers RS, Kleerebezem M.
  TITLE     Cre-lox-based system for multiple gene deletions and 
            selectable-marker removal in Lactobacillus plantarum
  JOURNAL   Appl. Environ. Microbiol. 73 (4), 1126-1135 (2007)
  PUBMED    17142375
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Lambert JM, Bongers RS, Kleerebezem M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2005) Health and Safety Department, NIZO Food 
            Research, Kernhemseweg 2, Ede, Gld 6718ZB, The Netherlands
REFERENCE   3  (bases 1 to 3737)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3737)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2007"; volume: "73"; issue: "4"; pages:
            "1126-1135"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-JUN-2005) Health and Safety Department, NIZO Food Research, 
            Kernhemseweg 2, Ede, Gld 6718ZB, The Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3737
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..648
                     /gene="cat"
                     /label=cat
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     protein_bind    772..805
                     /label=lox71
                     /note="Left element (LE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      complement(949..1050)
                     /label=Tlas
                     /note="Tlas"
                     /regulatory_class="terminator"
     CDS             complement(1165..1899)
                     /gene="ermBP"
                     /label=ermBP
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     rep_origin      2328..2873
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     regulatory      3285..3311
                     /label=TpepN
                     /note="TpepN"
                     /regulatory_class="terminator"
     protein_bind    3505..3538
                     /label=lox66
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      3609..3721
                     /label=P32
                     /note="P32"
                     /regulatory_class="promoter"
ORIGIN
        1 atgaacttta ataaaattga tttagacaat tggaagagaa aagagatatt taatcattat
       61 ttgaaccaac aaacgacttt tagtataacc acagaaattg atattagtgt tttataccga
      121 aacataaaac aagaaggata taaattttac cctgcattta ttttcttagt gacaagggtg
      181 ataaactcaa atacagcttt tagaactggt tacaatagcg acggagagtt aggttattgg
      241 gataagttag agccacttta tacaattttt gatggtgtat ctaaaacatt ctctggtatt
      301 tggactcctg taaagaatga cttcaaagag ttttatgatt tatacctttc tgatgtagag
      361 aaatataatg gttcggggaa attgtttccc aaaacaccta tacctgaaaa tgctttttct
      421 ctttctatta ttccatggac ttcatttact gggtttaact taaatatcaa taataatagt
      481 aattaccttc tacccattat tacagcagga aaattcatta ataaaggtaa ttcaatatat
      541 ttaccgctat ctttacaggt acatcattct gtttgtgatg gttatcatgc aggattgttt
      601 atgaactcta ttcaggaatt gtcagatagg cctaatgact ggcttttata atatgagata
      661 atgccgactg tactttcgga tcctaaacgc aattgatgat tggttcggaa ggcacgttag
      721 gaatcattac cgaagtaatc gttaaactgt tgccgattcc gctagggacc cataacttcg
      781 tataatgtat gctatacgaa cggtacagcc cgggcatgag ctccgatcgc tacgagaaga
      841 cgcactatcg accataccta ataatttatc tacattccct ttagtaacgt gaagaagatc
      901 tctaaagctg acggggtaaa ctatataaaa tccaaataaa tttctaaaaa taaaaaagtc
      961 tgtcgatgaa cagacttttt tattatagtt taaagcaaac ttttaaatat aataaaaaga
     1021 gttagttgaa attttctact aactcttttt tatttttagt ttttaactgc agaagcaaat
     1081 tcttctttag caaaagcttc atcgatgata gctttcaatt gagcgtgtaa ctttccaaat
     1141 ttacaaaagc gactcataga attatttcct cccgttaaat aatagataac tattaaaaat
     1201 agacaatact tgctcataag taacggtact taaattgttt actttggcgt gtttcattgc
     1261 ttgatgaaac tgatttttag taaacagttg acgatattct cgattgaccc attttgaaac
     1321 aaagtacgta tatagcttcc aatatttatc tggaacatct gtggtatggc gggtaagttt
     1381 tattaagaca ctgtttactt ttggtttagg atgaaagcat tccgctggca gcttaagcaa
     1441 ttgctgaatc gagacttgag tgtgcaagag caaccctagt gttcggtgaa tatccaaggt
     1501 acgcttgtag aatccttctt caacaatcag atagatgtca gacgcatggc tttcaaaaac
     1561 cactttttta ataatttgtg tgcttaaatg gtaaggaata ctcccaacaa ttttatacct
     1621 ctgtttgtta gggaattgaa actgtagaat atcttggtga attaaagtga cacgagtatt
     1681 cagttttaat ttttctgacg ataagttgaa tagatgactg tctaattcaa tagacgttac
     1741 ctgtttactt attttagcca gtttcgtcgt taaatgccct ttacctgttc caatttcgta
     1801 aacggtatcg gtttctttta aattcaattg ttttattatt tggttgagta ctttttcact
     1861 cgttaaaaag ttttgagaat attttatatt tttgttcatg taatcactcc ttcttaatta
     1921 caaattttta gcatctaatt taacttcaat tcctattata caaaatttta agatactgca
     1981 ctatcaacac actcttaagt ttgcttctaa gtcttatttc cataacttct tttacgtttc
     2041 cgccattctt tgctgtttcg atttttatga tatggtgcaa gtcagcacga acacgaaccg
     2101 tcttatctcc cattatatct ttttttgcac tgattggtgt atcatttcgt ttttcttttg
     2161 cggacctgca gatgcgatat catgcgcatg caagcttatc gatgataagc tgtcaaacat
     2221 gagaattaca acttatatcg tatggggctg acttcaggtg ctacatttga agagataaat
     2281 tgcactgaaa tctagaaata ttttatctga ttaataagat gatcttcttg agatcgtttt
     2341 ggtctgcgcg taatctcttg ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg
     2401 aaggttctct gagctaccaa ctctttgaac cgaggtaact ggcttggagg agcgcagtca
     2461 ccaaaacttg tcctttcagt ttagccttaa ccggcgcatg acttcaagac taactcctct
     2521 aaatcaatta ccagtggctg ctgccagtgg tgcttttgca tgtctttccg ggttggactc
     2581 aagacgatag ttaccggata aggcgcagcg gtcggactga acggggggtt cgtgcataca
     2641 gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacaaac
     2701 gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga
     2761 gggagccgcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact
     2821 gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct
     2881 ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca ggaaatctcc
     2941 gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt
     3001 gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg gtgcagcctt
     3061 ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac atagtaagcc
     3121 agtatacact ccgctagcgc tgatgtccgg cggtgctttt gccgttacgc accaccccgt
     3181 cagtagctga acaggaggga cagcgtgttg ctttgattga tagccaaaaa gcagcagttg
     3241 ataaagcaat tactgatatt gctgaaaaat tgtaatttat aaataaaaat caccttttag
     3301 aggtggtttt tttatttata aattattcgt ttgatttcgc tttcgataga acaatcaaag
     3361 cgagaataag gaagataaat cccataaggg cgggagcaga atgtccgaga ctaatgtaaa
     3421 tttgtccacc aattaaagga ccgataacgc gctcgagcct gatagaaaca gaagccactg
     3481 gagcacgttt aaacaattta aatctaccgt tcgtataatg tatgctatac gaagttatga
     3541 caatgtctta ggcgttaagg tcgttttagc cgatggtcgc gaagttaagt aaggtaccat
     3601 gcagtttaaa ttcggtcctc gggatatgat aagaatggct taataaagcg gttactttgg
     3661 atttttgtga gcttggacta gaaaaaaact tcacaaaatg ctatactagg tagataaaaa
     3721 tttaggaggc atatcaa
//
