pREX1LC vector (V003750)

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LOCUS       V003750                 9258 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003750
VERSION     V003750
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9258)
  AUTHORS   Murakawa M, Freeman MW.
  TITLE     Adeno-associated virus 2 (AAV)-based plasmid vector system that can
            mediate auto-excision of AAV terminal motifs via auto-regulated
            transient Rep78 expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9258)
  AUTHORS   Murakawa M, Freeman MW.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2001) Lipid Metabolism Unit, Massachusetts General
            Hospital, Jackson 1328, Boston, MA 02114-2694, USA
REFERENCE   3  (bases 1 to 9258)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9258)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAY-2001) Lipid Metabolism Unit, Massachusetts General Hospital,
            Jackson 1328, Boston, MA 02114-2694, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        69..372
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        373..576
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          1375..1727
                     /note="artificial"
     repeat_region   1421..1565
                     /label="adeno-associated virus 5' inverted terminal repeat"
                     /note="adeno-associated virus 5' inverted terminal repeat"
     misc_feature    1462..1503
                     /label="flip oriented DNA"
                     /note="flip oriented DNA"
     CDS             1728..2888
                     /gene="Rep52"
                     /label="Protein Rep52"
                     /note="Protein Rep52 from Adeno-associated virus 2 (isolate
                     Srivastava/1982). Accession#: Q89270"
     promoter        3352..4533
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             4552..5268
                     /label="hrGFP"
                     /note="humanized Renilla green fluorescent protein"
     polyA_signal    5385..5517
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     repeat_region   5612..5756
                     /label="adeno-associated virus 3' inverted terminal repeat"
                     /note="adeno-associated virus 3' inverted terminal repeat"
     misc_feature    5673..5715
                     /label="flop oriented DNA"
                     /note="flop oriented DNA"
     CDS             complement(5774..6901)
                     /label="HSV TK"
                     /note="herpes simplex virus thymidine kinase"
     misc_feature    complement(6905..6922)
                     /note="derived from bovine papilloma virus type 1; Region:
                     AU1 epitope"
     regulatory      6925..6934
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7152..7340
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     rep_origin      complement(7413..8001)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8175..9032)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9033..9137)
                     /label="AmpR promoter"