LOCUS       V003750                 9258 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003750
VERSION     V003750
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9258)
  AUTHORS   Murakawa M, Freeman MW.
  TITLE     Adeno-associated virus 2 (AAV)-based plasmid vector system that can
            mediate auto-excision of AAV terminal motifs via auto-regulated
            transient Rep78 expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9258)
  AUTHORS   Murakawa M, Freeman MW.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2001) Lipid Metabolism Unit, Massachusetts General
            Hospital, Jackson 1328, Boston, MA 02114-2694, USA
REFERENCE   3  (bases 1 to 9258)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9258)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAY-2001) Lipid Metabolism Unit, Massachusetts General Hospital,
            Jackson 1328, Boston, MA 02114-2694, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        69..372
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        373..576
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          1375..1727
                     /note="artificial"
     repeat_region   1421..1565
                     /label="adeno-associated virus 5' inverted terminal repeat"
                     /note="adeno-associated virus 5' inverted terminal repeat"
     misc_feature    1462..1503
                     /label="flip oriented DNA"
                     /note="flip oriented DNA"
     CDS             1728..2888
                     /gene="Rep52"
                     /label="Protein Rep52"
                     /note="Protein Rep52 from Adeno-associated virus 2 (isolate
                     Srivastava/1982). Accession#: Q89270"
     promoter        3352..4533
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             4552..5268
                     /label="hrGFP"
                     /note="humanized Renilla green fluorescent protein"
     polyA_signal    5385..5517
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     repeat_region   5612..5756
                     /label="adeno-associated virus 3' inverted terminal repeat"
                     /note="adeno-associated virus 3' inverted terminal repeat"
     misc_feature    5673..5715
                     /label="flop oriented DNA"
                     /note="flop oriented DNA"
     CDS             complement(5774..6901)
                     /label="HSV TK"
                     /note="herpes simplex virus thymidine kinase"
     misc_feature    complement(6905..6922)
                     /note="derived from bovine papilloma virus type 1; Region:
                     AU1 epitope"
     regulatory      6925..6934
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7152..7340
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     rep_origin      complement(7413..8001)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8175..9032)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9033..9137)
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacagat cttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
       61 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      121 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      181 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      241 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      301 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      361 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      421 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      481 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      541 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
      601 gcttactggc ttatcccgag tgagcacgca gggtctccat tttgaagcgg gaggtttgaa
      661 cgcgcagccg ccatgccggg gttttacgag attgtgatta aggtccccag cgaccttgac
      721 gggcatctgc ccggcatttc tgacagcttt gtgaactggg tggccgagaa ggaatgggag
      781 ttgccgccag attctgacat ggatctgaat ctgattgagc aggcacccct gaccgtggcc
      841 gagaagctgc agcgcgactt tctgacggaa tggcgccgtg tgagtaaggc cccggaggcc
      901 cttttctttg tgcaatttga gaagggagag agctacttcc acatgcacgt gctcgtggaa
      961 accaccgggg tgaaatccat ggttttggga cgtttcctga gtcagattcg cgaaaaactg
     1021 attcagagaa tttaccgcgg gatcgagccg actttgccaa actggttcgc ggtcacaaag
     1081 accagaaatg gcgccggagg cgggaacaag gtggtggatg agtgctacat ccccaattac
     1141 ttgctcccca agacccagcc tgaactgcag tgggcgtgga ctaatatgga acagtattta
     1201 agcgcctgtt tgaatctcac ggagcgtaaa cggttggtgg cgcagcatct gacgcacgtg
     1261 tcgcagacgc aggagcagaa caaagagaat cagaatccca attctgatgc gccggtgatc
     1321 agatcaaaaa cttcagccag gtacatggag ctggtcgggt ggctcgtgga caaggtaagc
     1381 tcgctaaaat cccctccatt cgcgtgtcct taagggaccc ttggccactc cctctctgcg
     1441 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     1501 ggcggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg
     1561 ttcctggagg ggtggagtcg tgacgtgaat tacgtcatag ggttagggag gtcctacgcg
     1621 tttgaaatgt tctcagtccc tggagggaag ggtaggtggg gctggcagga gatcaggcgt
     1681 ctagctccct ggggccctcc gtcgcggccc tcctggtctc tccctagggg attacctcgg
     1741 agaagcagtg gatacaggag gaccaggcct catacatctc cttcaatgcg gcctccaact
     1801 cgcggtccca aatcaaggct gccttggaca atgcgggaaa gattatgagc ctgactaaaa
     1861 ccgcccccga ctacctggtg ggccagcagc ccgtggagga catttccagc aatcggattt
     1921 ataaaatttt ggaactaaac gggtacgatc cccaatatgc ggcttccgtc tttctgggat
     1981 gggccacgaa aaagttcggc aagaggaaca ccatctggct gtttgggcct gcaactaccg
     2041 ggaagaccaa catcgcggag gccatagccc acactgtgcc cttctacggg tgcgtaaact
     2101 ggaccaatga gaactttccc ttcaacgact gtgtcgataa gatggtgatc tggtgggagg
     2161 aggggaagat gaccgccaag gtcgtggagt cggccaaagc cattctcgga ggaagcaagg
     2221 tgcgcgtgga ccagaaatgc aagtcctcgg cccagataga cccgactccc gtgatcgtca
     2281 cctccaacac caatatgtgc gccgtgattg acgggaactc aacgaccttc gaacaccagc
     2341 agccgttgca agaccggatg ttcaaatttg aactcacccg ccgtctggat catgactttg
     2401 ggaaggtcac caagcaggaa gtcaaagact ttttccggtg ggcaaaggat cacgtggttg
     2461 aggtggagca tgagttctac gtcaaaaagg gtggagccaa gaaaagaccc gcccccagtg
     2521 acgcagatat aagtgagccc aaacgggtgc gcgagtcagt tgcgcagcca tcgacgtcag
     2581 acgcggaagc ttcgatcaac tacgcagata gataccaaaa caaatgttct cgtcacgtgg
     2641 gcatgaatct gatgctgttt ccgtgcagac aatgcgagag aatgaatcag aactcaaata
     2701 tctgcttcac tcacggacag aaagactgtt tagagtgctt tcccgtgtca gaatctcaac
     2761 ccgtttctgt cgtcaaaaag gcgtatcaga aactgtgcta cattcatcat atcatgggaa
     2821 aggtgccaga cgcttgcact gcctgcgatc tggtcaatgt ggatttggat gactgcatct
     2881 ttgaacaata aatgcagctg tgaggaagcc caggccagct ctgaatccag cttctcagac
     2941 tgctgctttt gtgcctgcgt aatgagccag gaacttggaa tttctgcctt aaagggacca
     3001 agagatgtgg cacagccaca gttggaaggc agtatagccc tctgaaaacg ctgactcagc
     3061 ttggacagcg gaagacaaac gagagatatt ttctactgat agggaccatt atatttattt
     3121 atatatttat attttttaaa tatttattta tttatttatt tatttttgca actctattta
     3181 ttgagaatgt cttaccagaa taataaatta ttaaaacttt tgtttgtata gttatctggt
     3241 ttattttgaa aggggaaaaa tttgggcata ggtggagtgg gggagctatt gggatatggt
     3301 attgatgaga gtcaatgctg tttaaacctc tcttaaggta gcactagtga ggctccggtg
     3361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     3421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     3481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     3541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
     3601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacgc
     3661 ccctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt
     3721 tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
     3781 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
     3841 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
     3901 agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc
     3961 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
     4021 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
     4081 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
     4141 tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg
     4201 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca
     4261 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     4321 tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     4381 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     4441 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     4501 caaagttttt ttcttccatt tcaggtgtcg tgaaaagctt ggcgcgccac catggtgagc
     4561 aagcagatcc tgaagaacac cggcctgcag gagatcatga gcttcaaggt gaacctggag
     4621 ggcgtggtga acaaccacgt gttcaccatg gagggctgcg gcaagggcaa catcctgttc
     4681 ggcaaccagc tggtgcagat ccgcgtgacc aagggcgccc ccctgccctt cgccttcgac
     4741 atcctgagcc ccgccttcca gtacggcaac cgcaccttca ccaagtaccc cgaggacatc
     4801 agcgacttct tcatccagag cttccccgcc ggcttcgtgt acgagcgcac cctgcgctac
     4861 gaggacggcg gcctggtgga gatccgcagc gacatcaacc tgatcgagga gatgttcgtg
     4921 taccgcgtgg agtacaaggg ccgcaacttc cccaacgacg gccccgtgat gaagaagacc
     4981 atcaccggcc tgcagcccag cttcgaggtg gtgtacatga acgacggcgt gctggtgggc
     5041 caggtgatcc tggtgtaccg cctgaacagc ggcaagttct acagctgcca catgcgcacc
     5101 ctgatgaaga gcaagggcgt ggtgaaggac ttccccgagt accacttcat ccagcaccgc
     5161 ctggagaaga cctacgtgga ggacggcggc ttcgtggagc agcacgagac cgccatcgcc
     5221 cagctgacca gcctgggcaa gcccctgggc agcctgcacg agtgggtgta ataggcggcc
     5281 gcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     5341 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     5401 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5461 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggacct
     5521 gcaggtgaac tttggtctct gcgtatttct ttcttatcta gtttccatgg ctacgtagat
     5581 aagtagcatg gcgggttaat cattaactac aaggaacccc tagtgatgga gttggccact
     5641 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
     5701 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gagagggagt ggccaattaa
     5761 ttaagtcgac tcagttagcc tcccccatct cccgggcaaa cgtgcgcgcc aggtcgcaga
     5821 tcgtcggtat ggagccgggg gtggtgacgt gggtctggac catcccggag gtaagttgca
     5881 gcagggcgtc ccggcagccg gcgggcgatt ggtcgtaatc caggataaag acgtgcatgg
     5941 gacggaggcg tttggccaag acgtccaagg cccaggcaaa cacgttgtac aggtcgccgt
     6001 tgggggccag caactcgggg gcccgaaaca gggtaaataa cgtgtccccg atatggggtc
     6061 gtgggcccgc gttgctctgg ggctcggcac cctggggcgg cacggccgtc cccgaaagct
     6121 gtccccaatc ctcccgccac gacccgccgc cctgcagata ccgcaccgta ttggcaagca
     6181 gcccgtaaac gcggcgaatc gcggccagca tagccaggtc aagccgctcg ccggggcgct
     6241 ggcgtttggc caggcggtcg atgtgtctgt cctccggaag ggcccccaac acgatgtttg
     6301 tgccgggcaa ggtcggcggg atgagggcca cgaacgccag cacggcctgg ggggtcatgc
     6361 tgcccataag gtatcgcgcg gccgggtagc acaggagggc ggcgatggga tggcggtcga
     6421 agatgagggt gagggccggg ggcggggcat gtgagctccc agcctccccc ccgatatgag
     6481 gagccagaac ggcgtcggtc acggcataag gcatgcccat tgttatctgg gcgcttgtca
     6541 ttaccaccgc cgcgtccccg gccgatatct caccctggtc gaggcggtgt tgtgtggtgt
     6601 agatgttcgc gattgtctcg gaagccccca gcacctgcca gtaagtcatc ggctcgggta
     6661 cgtagacgat atcgtcgcgc gaacccaggg ccaccagcag ttgcgtggtg gtggttttcc
     6721 ccatcccgtg aggaccgtct atataaaccc gcagtagcgt gggcattttc tgctccaggc
     6781 ggacttccgt ggcttcttgc tgccggcgag ggcgcaacgc cgtacgtcgg ttgctatggc
     6841 cgcgagaacg cgcagcctgg tcgaacgcag acgcgtgttg atggcagggg tacgaagcca
     6901 tgttgatgta gcggtaggtg tcgcccatgg tggcggcctg cagctgtgtt cgaggccgcg
     6961 cgtgtcacct taatatgcga agtggacctg ggaccgcgcc gccccgactg catctgcgtg
     7021 ttcgagttcg ccaatgacaa gacgctgggc ggggagatcc ccctggatcc tcatgtttga
     7081 cagcttatct tcgataagct ataatgcggt agtttatcac agttaaattc ccccttacaa
     7141 ggaggaagag tatgactaag caagagaaga ctgctttgaa tatggctaga ttcattagat
     7201 cccaaactct gactttgtta gaaaagttga atgaattaga tgctgatgag caagctgata
     7261 tttgtgagag cttgcatgat catgcagatg aattgtatag atcctgtttg gctagatttg
     7321 gtgatgatgg tgagaatttg taaccgctga gcaataacta gcaccccgct tcggcggggt
     7381 ttttttattt aaatggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7441 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7501 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7561 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7621 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7681 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7741 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7801 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     7861 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7921 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7981 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8041 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8101 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8161 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8221 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8281 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8341 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8401 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8461 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt
     8521 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8581 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     8641 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8701 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8761 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
     8821 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     8881 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     8941 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9001 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9061 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9121 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     9181 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaactc
     9241 tcttaaggta gcgaattc
//
