pRESQ70 vector (V003752)

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LOCUS       40924_36778        7260 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pRESQ70, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Sivamani E, Qu R.
  TITLE     Expression enhancement of a rice polyubiquitin gene promoter
  JOURNAL   Plant Mol. Biol. 60 (2), 225-239 (2006)
  PUBMED    16429261
REFERENCE   2  (bases 1 to 7260)
  AUTHORS   Sivamani E, Starmer J, Qu R.
  TITLE     Sequence analysis of rice rubi3 promoter gene expression cassettes 
            for improved transgene expression
  JOURNAL   Plant Sci. 177 (6), 549-556 (2009)
REFERENCE   3  (bases 1 to 7260)
  AUTHORS   Sivamani E, Qu R.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-FEB-2008) Crop Science, North Carolina State 
            University, 101, Derieux Place, Raleigh, NC 27695, USA
REFERENCE   4  (bases 1 to 7260)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7260)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2006"; volume: "60"; issue: "2"; pages: "225-239"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Plant 
            Sci."; date: "2009"; volume: "177"; issue: "6"; pages: "549-556"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (20-FEB-2008) Crop Science, North Carolina State University, 101, 
            Derieux Place, Raleigh, NC 27695, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            325..2315
                     /gene="rubi3"
                     /label=rubi3
                     /note="polyubiquitin; from rice"
     regulatory      325..1108
                     /gene="rubi3"
                     /regulatory_class="promoter"
     exon            1109..1175
                     /gene="rubi3"
                     /number=1
     intron          1176..2315
                     /gene="rubi3"
                     /number=1
     CDS             2313..4124
                     /label=GUS
                     /note="beta-glucuronidase"
     terminator      4129..4380
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4389..4405)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4618..5073
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5355..5459
                     /label=AmpR promoter
     CDS             5460..6317
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6491..7079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"