LOCUS       40924_36778        7260 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pRESQ70, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Sivamani E, Qu R.
  TITLE     Expression enhancement of a rice polyubiquitin gene promoter
  JOURNAL   Plant Mol. Biol. 60 (2), 225-239 (2006)
  PUBMED    16429261
REFERENCE   2  (bases 1 to 7260)
  AUTHORS   Sivamani E, Starmer J, Qu R.
  TITLE     Sequence analysis of rice rubi3 promoter gene expression cassettes 
            for improved transgene expression
  JOURNAL   Plant Sci. 177 (6), 549-556 (2009)
REFERENCE   3  (bases 1 to 7260)
  AUTHORS   Sivamani E, Qu R.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-FEB-2008) Crop Science, North Carolina State 
            University, 101, Derieux Place, Raleigh, NC 27695, USA
REFERENCE   4  (bases 1 to 7260)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7260)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2006"; volume: "60"; issue: "2"; pages: "225-239"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Plant 
            Sci."; date: "2009"; volume: "177"; issue: "6"; pages: "549-556"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (20-FEB-2008) Crop Science, North Carolina State University, 101, 
            Derieux Place, Raleigh, NC 27695, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            325..2315
                     /gene="rubi3"
                     /label=rubi3
                     /note="polyubiquitin; from rice"
     regulatory      325..1108
                     /gene="rubi3"
                     /regulatory_class="promoter"
     exon            1109..1175
                     /gene="rubi3"
                     /number=1
     intron          1176..2315
                     /gene="rubi3"
                     /number=1
     CDS             2313..4124
                     /label=GUS
                     /note="beta-glucuronidase"
     terminator      4129..4380
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4389..4405)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4618..5073
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5355..5459
                     /label=AmpR promoter
     CDS             5460..6317
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6491..7079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 catgcctgca ggtcgactct agtaacggcc gccagtgtgc tggaattcgg cttagtcgac
      301 ccacccaacc ccatatcgac agaggatgtg aagaacaggt aaatcacgca gaagaaccca
      361 tctctgatag cagctatcga ttagaacaac gaatccatat tgggtccgtg ggaaatactt
      421 actgcacagg aaggggggcg atctgacgag gccccgccac cggcctcgac ccgaggccga
      481 ggccgacgaa gcgccggcga gtacggcgcc gcggcggcct ctgcccgtgc cctctgcgcg
      541 tgggagggag aggccgcggt ggtgggggcg cgcgcgcgcg cgcgcgcagc tggtgcggcg
      601 gcgcgggggt cagccgccga gccggcggcg acggaggagc agggcggcgt ggacgcgaac
      661 ttccgatcgg ttggtcagag tgcgcgagtt gggcttagcc aattaggtct caacaatcta
      721 ttgggccgta aaattcatgg gccctggttt gtctaggccc aatatcccgt tcatttcagc
      781 ccacaaatat ttccccagag gattattaag gcccacacgc agcttatagc agatcaagta
      841 cgatgtttcc tgatcgttgg atcggaaacg tacggtcttg atcaggcatg ccgacttcgt
      901 caaagagagg cggcatgacc tgacgcggag ttggttccgg gcaccgtctg gatggtcgta
      961 ccgggaccgg acacgtgtcg cgcctccaac tacatggaca cgtgtggtgc tgccattggg
     1021 ccgtacgcgt ggcggtgacc gcaccggatg ctgcctcgca ccgccttgcc cacgctttat
     1081 atagagaggt tttctctcca ttaatcgcat agcgagtcga atcgaccgaa ggggaggggg
     1141 agcgaagctt tgcgttctct aatcgcctcg tcaaggtaac taatcaatca cctcgtccta
     1201 atcctcgaat ctctcgtggt gcccgtctaa tctcgcgatt ttgatgctcg tggtggaaag
     1261 cgtaggagga tcccgtgcga gttagtctca atctctcagg gtttcgtgcg attttagggt
     1321 gatccacctc ttaatcgagt tacggtttcg tgcgatttta gggtaatcct cttaatctct
     1381 cattgattta gggtttcgtg agaatcgagg tagggatctg tgttatttat atcgatctaa
     1441 tagatggatt ggttttgaga ttgttctgtc agatggggat tgtttcgata tattacccta
     1501 atgatgtgtc agatggggat tgtttcgata tattacccta atgatgtgtc agatggggat
     1561 tgtttcgata tattacccta atgatggata ataagagtag ttcacagtta tgttttgatc
     1621 ctgccacata gtttgagttt tgtgatcaga tttagtttta cttatttgtg cttagttcgg
     1681 atgggattgt tctgatattg ttccaataga tgaatagctc gttaggttaa aatctttagg
     1741 ttgagttagg cgacacatag tttatttcct ctggatttgg attggaattg tgttcttagt
     1801 ttttttcccc tggatttgga ttggaattgt gtggagctgg gttagagaat tacatctgta
     1861 tcgtgtacac ctacttgaac tgtagagctt gggttctaag gtcaatttaa tctgtattgt
     1921 atctggctct ttgcctagtt gaactgtagt gctgatgttg tactgtgttt ttttacccgt
     1981 tttatttgct ttactcgtgc aaatcaaatc tgtcagatgc tagaactagg tggctttatt
     2041 ctgtgttctt acatagatct gttgtcctgt agttacttat gtcagttttg ttattatctg
     2101 aagatatttt tggttgttgc ttgttgatgt ggtgtgagct gtgagcagcg ctcttatgat
     2161 taatgatgct gtccaattgt agtgtagtat gatgtgattg atatgttcat ctattttgag
     2221 ctgacagtac cgatatcgta ggatctggtg ccaacttatt ctccagctgc ttttttttac
     2281 ctatgttaat tccaatcctt tcttgcctct tccagatgtt acgtcctgta gaaaccccaa
     2341 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg
     2401 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag
     2461 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt
     2521 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg
     2581 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg
     2641 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
     2701 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
     2761 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg
     2821 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
     2881 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca
     2941 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
     3001 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
     3061 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg
     3121 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct
     3181 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg
     3241 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
     3301 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
     3361 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggca
     3421 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact
     3481 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga
     3541 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
     3601 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct
     3661 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
     3721 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac
     3781 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata
     3841 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat
     3901 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat
     3961 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag
     4021 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga
     4081 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaatgagct cgttcaaaca
     4141 tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat
     4201 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta
     4261 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca
     4321 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc
     4381 gggaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     4441 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     4501 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
     4561 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
     4621 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4681 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4741 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     4801 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
     4861 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4921 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag
     4981 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     5041 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
     5101 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     5161 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     5221 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     5281 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     5341 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     5401 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     5461 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     5521 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     5581 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     5641 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     5701 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     5761 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     5821 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     5881 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     5941 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6001 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6061 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6121 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     6181 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     6241 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     6301 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     6361 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     6421 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6481 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6541 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6601 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     6661 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6721 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6781 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6841 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6901 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6961 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7021 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7081 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7141 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     7201 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
//
