pRES vector (V003759)

LOCUS       V003759                 8482 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003759
VERSION     V003759
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   Hu Q, Miao S, Ni X, Lu F, Yu H, Xing L, Jiang P.
  TITLE     Construction of a shuttle vector for use in Riemerella anatipestifer
  JOURNAL   J. Microbiol. Methods 95 (2), 262-267 (2013)
   PUBMED   24064367
REFERENCE   2  (bases 1 to 8482)
  AUTHORS   Hu Q, Miao S, Ni X, Lu F, Yu H, Xing L, Jiang P.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-2013) Poultry Diseases Research, Shanghai
            Veterinary Research Institute, CAAS, No. 518, Ziyue Road, Shanghai,
            Shanghai 200241, China
REFERENCE   3  (bases 1 to 8482)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8482)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Microbiol. Methods"; date: "2013"; volume: "95"; issue: "2"; pages:
            "262-267"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-AUG-2013) Poultry Diseases Research, Shanghai Veterinary
            Research Institute, CAAS, No. 518, Ziyue Road, Shanghai, Shanghai
            200241, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..266
                     /label="putative streptothricin resistance gene"
                     /note="putative streptothricin resistance gene"
                     /regulatory_class="promoter"
     primer_bind     complement(341..357)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(365..381)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(389..419)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(434..455)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(743..1331)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1505..2362)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2363..2467)
                     /label="AmpR promoter"
     CDS             complement(6049..6417)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(6450..6559)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             6858..7820
                     /gene="cfxA"
                     /label="Beta-lactamase"
                     /note="Beta-lactamase from Phocaeicola vulgatus.
                     Accession#: P30899"