LOCUS       V003759                 8482 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003759
VERSION     V003759
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   Hu Q, Miao S, Ni X, Lu F, Yu H, Xing L, Jiang P.
  TITLE     Construction of a shuttle vector for use in Riemerella anatipestifer
  JOURNAL   J. Microbiol. Methods 95 (2), 262-267 (2013)
   PUBMED   24064367
REFERENCE   2  (bases 1 to 8482)
  AUTHORS   Hu Q, Miao S, Ni X, Lu F, Yu H, Xing L, Jiang P.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-2013) Poultry Diseases Research, Shanghai
            Veterinary Research Institute, CAAS, No. 518, Ziyue Road, Shanghai,
            Shanghai 200241, China
REFERENCE   3  (bases 1 to 8482)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8482)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Microbiol. Methods"; date: "2013"; volume: "95"; issue: "2"; pages:
            "262-267"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-AUG-2013) Poultry Diseases Research, Shanghai Veterinary
            Research Institute, CAAS, No. 518, Ziyue Road, Shanghai, Shanghai
            200241, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..266
                     /label="putative streptothricin resistance gene"
                     /note="putative streptothricin resistance gene"
                     /regulatory_class="promoter"
     primer_bind     complement(341..357)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(365..381)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(389..419)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(434..455)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(743..1331)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1505..2362)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2363..2467)
                     /label="AmpR promoter"
     CDS             complement(6049..6417)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(6450..6559)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             6858..7820
                     /gene="cfxA"
                     /label="Beta-lactamase"
                     /note="Beta-lactamase from Phocaeicola vulgatus.
                     Accession#: P30899"
ORIGIN
        1 actagttaaa tgccactaac atatggtagc cgataattgc cggctttcgt gccaacttga
       61 tactttggtg cttttaacga accgaagtgc taaattgata cgaaagtgct gtaaatccgg
      121 caactacggc taccattttt cgttagcggg cattgtgaaa gataccagca taacagttaa
      181 acattgataa atgaaaaaaa aactactttg gatattaatt ttaggactga taataatcag
      241 ttgcaaacaa aggaaaacag aaatgctgca gagtcattct ctcgagctca gatctagagt
      301 cgacgctagc gggcccgcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
      361 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
      421 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
      481 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      541 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      601 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      661 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      721 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      781 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      841 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      901 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca
      961 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     1021 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1081 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1141 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     1201 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1261 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1321 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1381 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1441 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1501 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1561 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1621 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     1681 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1741 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1801 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1861 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1921 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     1981 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     2041 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     2101 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     2161 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     2221 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     2281 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     2341 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     2401 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2461 ttccgcgcac atttccccga aaagtgccac ctgcgatttg acaatcctga aataaacaga
     2521 aatgacttaa gggctatctt gttgaatagt ttagaaaacg acacggttat ttgggataga
     2581 aaacttgtta tgcttgaacc tggtaagaag aagtggacac taacttttga gaataaaccg
     2641 agtgaaacag cagatttggt tattcttgcc aatggcggga tgtccaaggt aagaaaattt
     2701 gttaccgaca cggaagttga agaaacaggt actttcaata tacaagccga tattcatcaa
     2761 ccagagataa actgtcctgg attttttcag ctatgcaatg gaaaccggct aatggcatct
     2821 caccaaggta atttattatt tgctaacccc aataataatg gtgcattgca ttttggaata
     2881 agttttaaaa cacctgatga atggaaaaac caaacgcagg tagattttca aaacagaaat
     2941 agtgtcgttg attttcttct gaaagaattt tccgattggg acgaacgcta caaagaattg
     3001 attcatacga cgttgtcatt tgtaggattg gctacacgga tatttccttt agaaaagcct
     3061 tggaaaagca agcgcccatt acccataaca atgattgggg atgccgcaca tttgatgccg
     3121 ccttttgcag ggcagggagt aaatagtggg ttggtggatg ccttgatatt gtctgataat
     3181 ctagccgatg gaaaatttaa tagcattgaa gaggctgtta aaaattatga acagcaaatg
     3241 tttatgtatg gcaaagaagc acaagaagaa tcaactcaaa acgaaattga aatgtttaaa
     3301 cccgacttta cgtttcagca attgttaaat gtataaaatg gaataaaacg gcacataaca
     3361 aacaaattgg caatagagcc ggtgaagtac ttctattgaa cttttgtgca atgttgaaga
     3421 ttagtaattc tattcaacat ttgtgctaaa agtcggctcc atcgccaatt tgccagccgt
     3481 tatgcggcag cttaaaaaaa gactacacca aaccaaaaaa atcatcttga caaccacccg
     3541 actttgaact acgaaggatg aaatttttca gggacaactt ccagcatttc caaaaaacag
     3601 tttaaccatt gacttggaga caaacaaaca atttgagcat taaggtttaa accgaatttt
     3661 tccgaaattg acctgacctg acttttcctg aaaatcgact ttaaagctgt ttttacagat
     3721 aaatcaggtt tctctaacag ataggaaata aatgctaagt atttggcttt aaacttaaaa
     3781 tcaaaaaata agtgttttct tttaatgttt aacagggctg atttgacagt tggcggtggc
     3841 aagaaacttt caggacctgt tgagtatact tgatcagatc tcgtatagag ctcgattaag
     3901 tttttatata gcattttttt tctaatactt ctttaattat taggagctat ttatccatcc
     3961 ccttttcttt agcttcaata aagtttcttt ctcggaacgc tccgattaat ttttcgtgaa
     4021 tatccaattg cttattgtaa gatagtctga aatattctat ttgtgttttt agagtatcca
     4081 aatgttcatt agacagttta atttgctctt tcaaaaaagg gtactctgtg atcctttttt
     4141 taaactcttg gtattcctct tctgtgaagt attcaatatg aggaaattct tcatttttat
     4201 cctgtcgttc gttgtcgttc gttgtcgcta aaatgtcgta atggtgtcgc tcggcaagaa
     4261 agaaaacaat gtcttctggt attgaaaagc cctttctgtc ggtatttgat atgttgtcgc
     4321 ttagtgtcgt tatgtgtcgc tggattgtcc gttcgctgac gccaaaaagc tctgcgattt
     4381 cttttgttgt atattttttc ataagcctat tgttgttctt ttttgaaatc tttaagcata
     4441 tttcttataa tcccaatcac ataagcagga gatatttttc ctttcttagt ccgacttttt
     4501 cttatctctt gaaaaaggaa gaaagaatag tttgatggaa gtgtagcctt acaatctttg
     4561 aatagggcat agttgtttaa taaaccttgt tgtgtaaaac caaattcgtt tgtaaaaaca
     4621 tcaatttctc tttgagttaa tacagagtgt atatcttttt cctcttctat tttgatcatc
     4681 ttcatacttt catctgcaaa ttgttcttgg aaaataggaa ttagagaaat aattttttta
     4741 tcctttgaaa gagtataatc aaaagtgtat ggagaacatt tatctaactc gtttttagcg
     4801 gggtctaata cacggcgttt aaacatactg atattaagat taccctgagg gttcctgtac
     4861 ttattttcca attgaaacat tttttcaaga aatgacactt catatgttat tggtttggtt
     4921 ttattagcta tcagttcgta aaaacgcatt gcataaatag tctcaaactg catagcaatc
     4981 ttcaattcat attttctcca tcctcttgta aaatccatta ccgcttcaac aattcttttg
     5041 tcagattgcc aagtaacatt tcttccatat ttatcaatcc aaaacttatc aataacacca
     5101 aagcaagcgt attctttttc atcttcgtac tcaatgttct ttgattttag gtctttgaat
     5161 gcttttttaa ttttataata atttttatct tcatcattag ctaacagcat agaaattgga
     5221 attgtatatt ttttatcccc ccataggtta gtatctattt tgatacaatc tttcaacgcc
     5281 tcattattta gcaatgcctg ttctatttct atttgcctat atagtatacg cttttcatag
     5341 actgaaaaat catatttagc cgtagtaaaa atataggact gaaacattag tttattatct
     5401 acttttgcca tatatagaga gttatgaata taaaatgcac ttttagaata caaatatata
     5461 aaaaatgaac ttactaaaaa gcaagtttaa aaaagtattc taaaagttca aaaaaagtat
     5521 tctaaaagtt catttttgga gctaaaaaga ctaataaagt attctaaaag ttcattattt
     5581 ttatataact tcctatttat taaatagttc cgaggattgg tagatttgtc ttttagtagt
     5641 ttattttaaa ggtaatagaa ataagtttaa tgttaataag tttggttttg tataatattg
     5701 aaaatcaata gtttataaat ttgagtttat tttaaaagta ttctaaaagt tcacgaatga
     5761 aaaaaaacaa acaaaaagca tagcaagaat tttctaacac tgggtgatat tatgtatagc
     5821 atagtaaatc ataatcactt gatatttaat atcttatgat ttaattattt tttaagtcag
     5881 gttaaaaaga gggctaatgc ctaaatagga attcggcgct cggtcttgcc ttgctcgtcg
     5941 gtgatgtact tcaccagctc cgcgaagtcg ctcttcttga tggagcgcat ggggacgtgc
     6001 ttggcaatca cgcgcacccc ccggccgttt tagcggctaa aaaagtcatg gctctgccct
     6061 cgggcggacc acgcccatca tgaccttgcc aagctcgtcc tgcttctctt cgatcttcgc
     6121 cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg cgcgggtcgt cggtgagcca
     6181 gagtttcagc aggccgccca ggcggcccag gtcgccattg atgcgggcca gctcgcggac
     6241 gtgctcatag tccacgacgc ccgtgatttt gtagccctgg ccgacggcca gcaggtaggc
     6301 cgacaggctc atgccggccg ccgccgcctt ttcctcaatc gctcttcgtt cgtctggaag
     6361 gcagtacacc ttgataggtg ggctgccctt cctggttggc ttggtttcat cagccatccg
     6421 cttgccctca tctgttacgc cggcggtagc cggccagcct cgcagagcag gattcccgtt
     6481 gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg cgggtgggcc
     6541 tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt ctacacgaac
     6601 cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat
     6661 aatgaccccg aagcagggtt atgcagcgga aaaattcggg ggatccccaa aatcagttct
     6721 ttagcgatta ctaatttaca aagaaaattc gacaaactgt tatttttcta tctatttatt
     6781 tgggtgggaa actttagtta tgtacctttg tcggcaaata aagatattct cgtcaaacaa
     6841 atataaataa tataaacatg gaaaaaaaca gaaaaaaaca aatcgtagtt ttgagtatag
     6901 ctttagtttg cattttcatc ttggtatttt cattgttcca taaatcagcg acaaaagata
     6961 gcgcaaatcc tcctttaaca aatgttttga ctgatagcat ttctcaaatt gtctcagctt
     7021 gtcctggcga aattggtgtg gcggttattg ttaataacag agatacggtt aaggtcaata
     7081 ataagagtgt ttatcctatg atgagtgtgt ttaaggttca tcaggcatta gctctttgta
     7141 atgactttga caataaagga atttcacttg ataccttagt aaatataaat agggataaac
     7201 ttgacccaaa gacttggagt cctatgctga aagattattc agggccagtc atatcattga
     7261 cagtgagaga tttgctgcgt tatactctta ctcagagtga caacaatgca agcaacctta
     7321 tgtttaagga tatggttaat gtcgctcaaa cagatagttt tatagccaca ctcattcctc
     7381 gttcaagttt tcagatagct tatacggaag aggaaatgtc ggctgaccat aacaaggctt
     7441 actctaacta tacatctcct cttggtgctg caatgttgat gaatcgtttg tttactgaag
     7501 gtcttatcga tgatgagaaa caaagtttca ttaagaatac gttaaaagaa tgcaaaacag
     7561 gtgtagatag gatagcagct ccacttcttg ataaagaagg ggttgttata gcgcataaga
     7621 caggttcagg ttatgttaat gaaaatggtg ttcttgcagc tcacaatgat gttgcctata
     7681 tatgtctgcc taataatatc agttatacct tagcggtatt tgttaaggat ttcaagggaa
     7741 ataaatcaca agcgtcacaa tatgttgcgc atatatcagc tgtagtatat tctttattaa
     7801 tgcaaacttc agtaaaatct taaactgcac ttgctttgat aattaatgat aaacaatcta
     7861 aaagcactct aatcgttatc ggagtgcttt tagattacta atcaaattgc ttctattata
     7921 atttgaatcc tctacttttt ctttcttcct gttgcggcac tcttgctcca tatctaagcc
     7981 tgtgccattg ctccctgaac cagtcggcaa tcggcttcct gtttattgtc aggttcagcc
     8041 tgctttcatc gtcagggtca ttttccaccc ttaaaatatc atcctttata tcaaagttcc
     8101 gcctgtgttg ctcggaatag attttaccgc tacatttcag ggcttctttt ttgactataa
     8161 gactttcagt catttcttta gagaatccca gcatggcaca aaacttttcc atgcgcagca
     8221 tttccctgaa catcggaaac cacctgaaag ccttgtctat gattctggcg agccgtgaca
     8281 gttctttttc tttcatgtca agttcctgcc tgtgcatttc tctgagattg tggatttgcg
     8341 tatatgctgt ttctgacatc tgctgtactt ggctctgcaa tttttcaata ttggtgttcc
     8401 gttttgaaac ctcgtcttgc agtttttcgt tggcacgttc cagttctttc agttttccac
     8461 tcccgaaaag agaagcaact cc
//
