pCPT8 vector (V008213)

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LOCUS       40924_13175        3012 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCPT8, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Pitulle C, Pace NR.
  TITLE     Novel T-cloning vector for plasmid-based 16S rDNA analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Pitulle C.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 3012)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3012)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-SEP-1998) Plant "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..136
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    1
                     /label=T7 RNA polymerase initiation site
                     /note="T7 RNA polymerase initiation site"
     misc_feature    15..29
                     /label=XcmI restriction site
                     /note="XcmI restriction site"
     misc_feature    32..46
                     /label=XcmI restriction site
                     /note="XcmI restriction site"
     promoter        complement(134..152)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(170..186)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(194..210)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(218..248)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(263..284)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(572..1160)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1334..2191)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2192..2296)
                     /label=AmpR promoter
     rep_origin      complement(2377..2832)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2973..2989
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2996..3012
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"