LOCUS       40924_13175        3012 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCPT8, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Pitulle C, Pace NR.
  TITLE     Novel T-cloning vector for plasmid-based 16S rDNA analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Pitulle C.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 3012)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3012)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-SEP-1998) Plant "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..136
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    1
                     /label=T7 RNA polymerase initiation site
                     /note="T7 RNA polymerase initiation site"
     misc_feature    15..29
                     /label=XcmI restriction site
                     /note="XcmI restriction site"
     misc_feature    32..46
                     /label=XcmI restriction site
                     /note="XcmI restriction site"
     promoter        complement(134..152)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(170..186)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(194..210)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(218..248)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(263..284)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(572..1160)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1334..2191)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2192..2296)
                     /label=AmpR promoter
     rep_origin      complement(2377..2832)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2973..2989
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2996..3012
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggccccagaa ttcatatgga tccaggtaac caatggcatg gccgcgggat
       61 atcactagtg cggccgcctg caggtcgacc atatgggaga gctcccaacg cgttggatgc
      121 atagcttgag tattctatag tgtcacctaa atagcttggc gtaatcatgg tcatagctgt
      181 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
      241 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
      301 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
      361 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
      421 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
      481 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
      541 ggaaccgtaa aaaggccgcg ttgctggcgt ttttcgatag gctccgcccc cctgacgagc
      601 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      661 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
      721 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
      781 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
      841 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
      901 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
      961 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     1021 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     1081 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1141 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     1201 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     1261 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     1321 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     1381 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     1441 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     1501 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     1561 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     1621 gtttgcgcaa cgttgttggc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     1681 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     1741 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     1801 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     1861 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatac cgcgcccggc
     1921 gaccgagttg ctcttgcccg gcgtcaatac gggataatag tgtatgacat agcagaactt
     1981 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     2041 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     2101 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     2161 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     2221 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     2281 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgtatgcggt gtgaaatacc
     2341 gcacagatgc gtaaggagaa aataccgcat caggcgaaat tgtaaacgtt aatattttgt
     2401 taaaattcgc gttaaatatt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     2461 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     2521 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     2581 atcagggcga tggcccacta cgtgaaccat cacccaaatc aagttttttg cggtcgaggt
     2641 gccgtaaagc tctaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     2701 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     2761 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     2821 tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     2881 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     2941 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata
     3001 cgactcacta ta
//
