pCPP5386 vector (V008215)

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LOCUS       V008215                11865 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008215
VERSION     V008215
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11865)
  AUTHORS   Kvitko BH, Ramos AR, Morello JE, Oh HS, Collmer A.
  TITLE     Identification of harpins in Pseudomonas syringae pv. tomato DC3000,
            which are functionally similar to HrpK1 in promoting translocation
            of type III secretion system effectors
  JOURNAL   J. Bacteriol. 189 (22), 8059-8072 (2007)
   PUBMED   17873033
REFERENCE   2  (bases 1 to 11865)
  AUTHORS   Kvitko BH, Ramos AR, Morello JE, Oh H-S., Collmer A.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2007) Plant Pathology, Cornell University, 334
            Plant Science, Ithaca, NY 14853, USA
REFERENCE   3  (bases 1 to 11865)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11865)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "2007"; volume: "189"; issue: "22"; pages:
            "8059-8072"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-JUL-2007) Plant Pathology, Cornell University, 334 Plant
            Science, Ithaca, NY 14853, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..712)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"
     misc_feature    complement(707..860)
                     /note="similar to plasmid ColE1 in GenBank Accession Number
                     NC_001371"
     misc_feature    complement(852..1082)
                     /note="similar to plasmid ColE1 in GenBank Accession Number
                     NC_001371"
     primer_bind     1222..1238
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    1268..1292
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1312..2004)
                     /gene="SCEI"
                     /label="Intron-encoded endonuclease I-SceI"
                     /note="Intron-encoded endonuclease I-SceI from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P03882"
     RBS             complement(2020..2040)
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(2052..2076)
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      complement(2175..2224)
                     /label="PnptII"
                     /note="PnptII"
                     /regulatory_class="promoter"
     primer_bind     complement(2483..2499)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    2507..2523
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2531..2561)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2576..2597)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2832..3200)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(3233..3342)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     misc_feature    3474..3707
                     /note="similar to plasmid ColE1 in GenBank Accession Number
                     NC_001371"
     misc_feature    3699..3851
                     /note="similar to plasmid ColE1 in GenBank Accession Number
                     NC_001371"
     CDS             complement(4026..4673)
                     /label="TetR"
                     /note="tetracycline resistance regulatory protein"
     CDS             4779..5975
                     /label="TcR"
                     /note="tetracycline efflux protein"
     CDS             complement(6609..7274)
                     /codon_start=1
                     /product="unknown plasmid factor 16.5"
                     /label="unknown plasmid factor 16.5"
                     /protein_id="ABS87310.1"
                     /translation="MTWCIANARALWGQFRLGVQQPALYWHFRNKRALLDALASLSIAR
                     AHGALYELPINRGLKLTIVIKAQIRRLGAADVQDFREIRLSALKKAPEMFGSVYEHEEK
                     KPMEAFAERLRDAVAFGAYIDGEIIGLSVFKQEDGPKDAHKAHLSGVFVEPEQRGRGVA
                     GMLLRALPAGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGSRRR"
     CDS             complement(7252..8397)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(8446..8796)
                     /codon_start=1
                     /gene="ssb"
                     /product="single-stranded DNA binding protein"
                     /label="ssb"
                     /protein_id="ABS87312.1"
                     /translation="MSHNQFQFIGNLTRDTEVRHGNSNKPQAIFDIAVNEEWRNDAGDK
                     QERTDFFRIKCFGSQAEAHGKYLGKGSLVFVQGKIRNTKYEKDGQTVYGTDFIADKVDY
                     LDTKAPGGSNQE"
     gene            complement(8446..8796)
                     /gene="ssb"
                     /label="ssb"
     CDS             8917..9282
                     /codon_start=1
                     /gene="trbA"
                     /product="TrbA"
                     /label="trbA"
                     /protein_id="ABS87313.1"
                     /translation="MYNQIFFTNILRLLDERGMTKHELSERAGVSISFLSDLTNGKANP
                     SLKVMEAIADALETPLPLLLESTDLDREALAEIAGHPFKSSVPPGYERISVVLPSHKAF
                     IVKKWGDDTRKKLRGRL"
     gene            8917..9282
                     /gene="trbA"
                     /label="trbA"
     CDS             complement(10320..11414)
                     /codon_start=1
                     /gene="incC1"
                     /product="IncC1"
                     /label="incC1"
                     /protein_id="ABS87314.1"
                     /translation="MGVIHEETAYRKPVPGGDPGAGSGAADHRDSAGRLSRWEATGDVR
                     NVAGTDQGRSVASGASRVGRVRGQELARGVRAGNGGSAGTSGVHRPEVGSGRQEKTGNQ
                     TMKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNASYTLKDFATGLHAS
                     KLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDARELFGANIKALAN
                     QGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMVTTIANVRQKNAKL
                     QFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADALASGVPVWKIKKT
                     AARKASKEVRALADYVFTKMEISQ"
     gene            complement(10320..11414)
                     /gene="incC1"
                     /label="incC1"
     CDS             complement(10320..11099)
                     /codon_start=1
                     /gene="incC2"
                     /product="IncC2"
                     /label="incC2"
                     /protein_id="ABS87315.1"
                     /translation="MKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNAS
                     YTLKDFATGLHASKLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDA
                     RELFGANIKALANQGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMV
                     TTIANVRQKNAKLQFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADA
                     LASGVPVWKIKKTAARKASKEVRALADYVFTKMEISQ"
     gene            complement(10320..11099)
                     /gene="incC2"
                     /label="incC2"
     CDS             complement(11096..11401)
                     /codon_start=1
                     /gene="korA"
                     /product="KorA"
                     /label="korA"
                     /protein_id="ABS87316.1"
                     /translation="MKKRLTESQFQEAIQGLEVGQQTIEIARGVLVDGKPQATFATSLG
                     LTRGAVSQAVHRVWAAFEDKNLPEGYARVTAVLPEHQAYIVRKWEADAKKKQETKR"
     gene            complement(11096..11401)
                     /gene="korA"
                     /label="korA"