LOCUS       V008215                11865 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008215
VERSION     V008215
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11865)
  AUTHORS   Kvitko BH, Ramos AR, Morello JE, Oh HS, Collmer A.
  TITLE     Identification of harpins in Pseudomonas syringae pv. tomato DC3000,
            which are functionally similar to HrpK1 in promoting translocation
            of type III secretion system effectors
  JOURNAL   J. Bacteriol. 189 (22), 8059-8072 (2007)
   PUBMED   17873033
REFERENCE   2  (bases 1 to 11865)
  AUTHORS   Kvitko BH, Ramos AR, Morello JE, Oh H-S., Collmer A.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2007) Plant Pathology, Cornell University, 334
            Plant Science, Ithaca, NY 14853, USA
REFERENCE   3  (bases 1 to 11865)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11865)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "2007"; volume: "189"; issue: "22"; pages:
            "8059-8072"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-JUL-2007) Plant Pathology, Cornell University, 334 Plant
            Science, Ithaca, NY 14853, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..712)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"
     misc_feature    complement(707..860)
                     /note="similar to plasmid ColE1 in GenBank Accession Number
                     NC_001371"
     misc_feature    complement(852..1082)
                     /note="similar to plasmid ColE1 in GenBank Accession Number
                     NC_001371"
     primer_bind     1222..1238
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    1268..1292
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1312..2004)
                     /gene="SCEI"
                     /label="Intron-encoded endonuclease I-SceI"
                     /note="Intron-encoded endonuclease I-SceI from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P03882"
     RBS             complement(2020..2040)
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(2052..2076)
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      complement(2175..2224)
                     /label="PnptII"
                     /note="PnptII"
                     /regulatory_class="promoter"
     primer_bind     complement(2483..2499)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    2507..2523
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2531..2561)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2576..2597)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2832..3200)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(3233..3342)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     misc_feature    3474..3707
                     /note="similar to plasmid ColE1 in GenBank Accession Number
                     NC_001371"
     misc_feature    3699..3851
                     /note="similar to plasmid ColE1 in GenBank Accession Number
                     NC_001371"
     CDS             complement(4026..4673)
                     /label="TetR"
                     /note="tetracycline resistance regulatory protein"
     CDS             4779..5975
                     /label="TcR"
                     /note="tetracycline efflux protein"
     CDS             complement(6609..7274)
                     /codon_start=1
                     /product="unknown plasmid factor 16.5"
                     /label="unknown plasmid factor 16.5"
                     /protein_id="ABS87310.1"
                     /translation="MTWCIANARALWGQFRLGVQQPALYWHFRNKRALLDALASLSIAR
                     AHGALYELPINRGLKLTIVIKAQIRRLGAADVQDFREIRLSALKKAPEMFGSVYEHEEK
                     KPMEAFAERLRDAVAFGAYIDGEIIGLSVFKQEDGPKDAHKAHLSGVFVEPEQRGRGVA
                     GMLLRALPAGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGSRRR"
     CDS             complement(7252..8397)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(8446..8796)
                     /codon_start=1
                     /gene="ssb"
                     /product="single-stranded DNA binding protein"
                     /label="ssb"
                     /protein_id="ABS87312.1"
                     /translation="MSHNQFQFIGNLTRDTEVRHGNSNKPQAIFDIAVNEEWRNDAGDK
                     QERTDFFRIKCFGSQAEAHGKYLGKGSLVFVQGKIRNTKYEKDGQTVYGTDFIADKVDY
                     LDTKAPGGSNQE"
     gene            complement(8446..8796)
                     /gene="ssb"
                     /label="ssb"
     CDS             8917..9282
                     /codon_start=1
                     /gene="trbA"
                     /product="TrbA"
                     /label="trbA"
                     /protein_id="ABS87313.1"
                     /translation="MYNQIFFTNILRLLDERGMTKHELSERAGVSISFLSDLTNGKANP
                     SLKVMEAIADALETPLPLLLESTDLDREALAEIAGHPFKSSVPPGYERISVVLPSHKAF
                     IVKKWGDDTRKKLRGRL"
     gene            8917..9282
                     /gene="trbA"
                     /label="trbA"
     CDS             complement(10320..11414)
                     /codon_start=1
                     /gene="incC1"
                     /product="IncC1"
                     /label="incC1"
                     /protein_id="ABS87314.1"
                     /translation="MGVIHEETAYRKPVPGGDPGAGSGAADHRDSAGRLSRWEATGDVR
                     NVAGTDQGRSVASGASRVGRVRGQELARGVRAGNGGSAGTSGVHRPEVGSGRQEKTGNQ
                     TMKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNASYTLKDFATGLHAS
                     KLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDARELFGANIKALAN
                     QGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMVTTIANVRQKNAKL
                     QFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADALASGVPVWKIKKT
                     AARKASKEVRALADYVFTKMEISQ"
     gene            complement(10320..11414)
                     /gene="incC1"
                     /label="incC1"
     CDS             complement(10320..11099)
                     /codon_start=1
                     /gene="incC2"
                     /product="IncC2"
                     /label="incC2"
                     /protein_id="ABS87315.1"
                     /translation="MKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNAS
                     YTLKDFATGLHASKLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDA
                     RELFGANIKALANQGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMV
                     TTIANVRQKNAKLQFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADA
                     LASGVPVWKIKKTAARKASKEVRALADYVFTKMEISQ"
     gene            complement(10320..11099)
                     /gene="incC2"
                     /label="incC2"
     CDS             complement(11096..11401)
                     /codon_start=1
                     /gene="korA"
                     /product="KorA"
                     /label="korA"
                     /protein_id="ABS87316.1"
                     /translation="MKKRLTESQFQEAIQGLEVGQQTIEIARGVLVDGKPQATFATSLG
                     LTRGAVSQAVHRVWAAFEDKNLPEGYARVTAVLPEHQAYIVRKWEADAKKKQETKR"
     gene            complement(11096..11401)
                     /gene="korA"
                     /label="korA"
ORIGIN
        1 ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga
       61 ggcccgcgtt agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc
      121 ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa
      181 gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg
      241 gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca
      301 ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag
      361 tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg
      421 taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat
      481 cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg
      541 tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg
      601 ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg gccatagacg
      661 gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg tcggaaaggc gctcttccgc
      721 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      781 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      841 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      901 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      961 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1021 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1081 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     1141 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     1201 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attcgagctc ggtacccggg
     1261 gatcatcacc actttgtaca agaaagctgg gtcggcgcgc ccaccctttt atttcaggaa
     1321 agtttcggag gagatagtgt tcggcagttt gtacatcatc tgcgggatca ggtacggttt
     1381 gatcaggttg tagaagatca ggtaagacat agaatcgatg tagatgatcg gtttgttttt
     1441 gttgattttt acgtaacagt tcagttggaa tttgttacgc agacccttaa ccaggtattc
     1501 tacttcttcg aaagtgaaag actgggtgtt cagtacgatc gatttgttgg tagagttttt
     1561 gttgtaatcc catttaccac catcatccat gaaccagtat gccagagaca tcggggtcag
     1621 gtagttttca accaggttgt tcgggatggt ttttttgttg ttaacgatga acaggttagc
     1681 cagtttgttg aaagcttggt gtttgaaagt ctgggcgccc caggtgatta ccaggttacc
     1741 caggtggtta acacgttctt ttttgtgcgg cggggacagt acccactgat cgtacagcag
     1801 acatacgtgg tccatgtatg ctttgttttt ccactcgaac tgcatacagt aggttttacc
     1861 ttcatcacga gaacggatgt aagcatcacc caggatcaga ccgatacctg cttcgaactg
     1921 ttcgatgttc agttcgatca gctgggattt gtattctttc agcagtttag agttcggacc
     1981 caggttcatt acctggtttt tttgatgcat ggtgaagggc tccttcttaa agttaaacaa
     2041 ggcggccgcg gagcctgctt ttttgtacaa acttgtgatg atcctctaga gtcgacggat
     2101 ccccgggaat tcccggggat cttgatcccc tgcgccatca gatccttggc ggcaagaaag
     2161 ccatccagtt tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctggcaatt
     2221 ccggttcgct tgctgtccat aaaaccgccc agtctagcta tcgccatgta agcccactgc
     2281 aagctacctg ctttctcttt gcgcttgcgt tttcccttgt ccagatagcc cagtagctga
     2341 cattcatccg gggtcagcac cgtttctgcg gactggcttt ctacgtgttc cgcttccttt
     2401 agcagccctt gcgccctgag tgcttgcggc agcgtgaagc tgatccgtcg acctgcaggc
     2461 atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     2521 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     2581 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     2641 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     2701 gctcggtctt gccttgctcg tcggtgatgt acttcaccag ctccgcgaag tcgctcttct
     2761 tgatggagcg catggggacg tgcttggcaa tcacgcgcac cccccggccg ttttagcggc
     2821 taaaaaagtc atggctctgc cctcgggcgg accacgccca tcatgacctt gccaagctcg
     2881 tcctgcttct cttcgatctt cgccagcagg gcgaggatcg tggcatcacc gaaccgcgcc
     2941 gtgcgcgggt cgtcggtgag ccagagtttc agcaggccgc ccaggcggcc caggtcgcca
     3001 ttgatgcggg ccagctcgcg gacgtgctca tagtccacga cgcccgtgat tttgtagccc
     3061 tggccgacgg ccagcaggta ggccgacagg ctcatgccgg ccgccgccgc cttttcctca
     3121 atcgctcttc gttcgtctgg aaggcagtac accttgatag gtgggctgcc cttcctggtt
     3181 ggcttggttt catcagccat ccgcttgccc tcatctgtta cgccggcggt agccggccag
     3241 cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag
     3301 gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgacg ccgttggata
     3361 caccaaggaa agtctacacg aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat
     3421 ggatataccg aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc ggaaaagcgc
     3481 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3541 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3601 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3661 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3721 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     3781 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     3841 ggaagagcgc cagaaggccg ccagagaggc cgagcgcggc cgtgaggctt ggacgctagg
     3901 gcagggcatg aaaaagcccg tagcgggctg ctacgggcgt ctgacgcggt ggaaaggggg
     3961 aggggatgtt gtctacatgg ctctgctgta gtgagtgggt tgcgctccgg cagcggtcct
     4021 gatcaatcgt caccctttct cggtccttca acgttcctga caacgagcct ccttttcgcc
     4081 aatccatcga caatcaccgc gagtccctgc tcgaacgctg cgtccggacc ggcttcgtcg
     4141 aaggcgtcta tcgcggcccg caacagcggc gagagcggag cctgttcaac ggtgccgccg
     4201 cgctcgccgg catcgctgtc gccggcctgc tcctcaagca cggccccaac agtgaagtag
     4261 ctgattgtca tcagcgcatt gacggcgtcc ccggccgaaa aacccgcctc gcagaggaag
     4321 cgaagctgcg cgtcggccgt ttccatctgc ggtgcgcccg gtcgcgtgcc ggcatggatg
     4381 cgcgcgccat cgcggtaggc gagcagcgcc tgcctgaagc tgcgggcatt cccgatcaga
     4441 aatgagcgcc agtcgtcgtc ggctctcggc accgaatgcg tatgattctc cgccagcatg
     4501 gcttcggcca gtgcgtcgag cagcgcccgc ttgttcctga agtgccagta aagcgccggc
     4561 tgctgaaccc ccaaccgttc cgccagtttg cgtgtcgtca gaccgtctac gccgacctcg
     4621 ttcaacaggt ccagggcggc acggatcact gtattcggct gcaactttgt catgcttgac
     4681 actttatcac tgataaacat aatatgtcca ccaacttatc agtgataaag aatccgcgcg
     4741 ttcaatcgga ccagcggagg ctggtccgga ggccagacat gaaacccaac atacccctga
     4801 tcgtaattct gagcactgtc gcgctcgacg ctgtcggcat cggcctgatt atgccggtgc
     4861 tgccgggcct cctgcgcgat ctggttcact cgaacgacgt caccgcccac tatggcattc
     4921 tgctggcgct gtatgcgttg gtgcaatttg cctgcgcacc tgtgctgggc gcgctgtcgg
     4981 atcgtttcgg gcggcggcca atcttgctcg tctcgctggc cggcgccact gtcgactacg
     5041 ccatcatggc gacagcgcct ttcctttggg ttctctatat cgggcggatc gtggccggca
     5101 tcaccggggc gactggggcg gtagccggcg cttatattgc cgatatcact gatggcgatg
     5161 agcgcgcgcg gcacttcggc ttcatgagcg cctgtttcgg gttcgggatg gtcgcgggac
     5221 ctgtgctcgg tgggctgatg ggcggtttct ccccccacgc tccgttcttc gccgcggcag
     5281 ccttgaacgg cctcaatttc ctgacgggct gtttcctttt gccggagtcg cacaaaggcg
     5341 aacgccggcc gttacgccgg gaggctctca acccgctcgc ttcgttccgg tgggcccggg
     5401 gcatgaccgt cgtcgccgcc ctgatggcgg tcttcttcat catgcaactt gtcggacagg
     5461 tgccggccgc gctttgggtc attttcggcg aggatcgctt tcactgggac gcgaccacga
     5521 tcggcatttc gcttgccgca tttggcattc tgcattcact cgcccaggca atgatcaccg
     5581 gccctgtagc cgcccggctc ggcgaaaggc gggcactcat gctcggaatg attgccgacg
     5641 gcacaggcta catcctgctt gccttcgcga cacggggatg gatggcgttc ccgatcatgg
     5701 tcctgcttgc ttcgggtggc atcggaatgc cggcgctgca agcaatgttg tccaggcagg
     5761 tggatgagga acgtcagggg cagctgcaag gctcactggc ggcgctcacc agcctgacct
     5821 cgatcgtcgg acccctcctc ttcacggcga tctatgcggc ttctataaca acgtggaacg
     5881 ggtgggcatg gattgcaggc gctgccctct acttgctctg cctgccggcg ctgcgtcgcg
     5941 ggctttggag cggcgcaggg caacgagccg atcgctgatc gtggaaacga taggcctatg
     6001 ccatgcgggt caaggcgact tccggcaagc tatacgcgcc ctaggagtgc ggttggaacg
     6061 ttggcccagc cagatactcc cgatcacgag caggacgccg atgatttgaa gcgcactcag
     6121 cgtctgatcc aagaacaacc atcctagcaa cacggcggtc cccgggctga gaaagcccag
     6181 taaggaaaca actgtaggtt cgagtcgcga gatcccccgg aaccaaagga agtaggttaa
     6241 acccgctccg atcaggccga gccacgccag gccgagaaca ttggttcctg taggcatcgg
     6301 gattggcgga tcaaacacta aagctactgg aacgagcaga agtcctccgg ccgccagttg
     6361 ccaggcggta aaggtgagca gaggcacggg aggttgccac ttgcgggtca gcacggttcc
     6421 gaacgccatg gaaaccgccc ccgccaggcc cgctgcgacg ccgacaggat ctagcgctgc
     6481 gtttggtgtc aacaccaaca gcgccacgcc cgcagttccg caaatagccc ccaggaccgc
     6541 catcaatcgt atcgggctac ctagcagagc ggcagagatg aacacgacca tcagcggctg
     6601 cacagcgcct accgtcgccg cgacccgccc ggcaggcggt agaccgaaat aaacaacaag
     6661 ctccagaata gcgaaatatt aagtgcgccg aggatgaaga tgcgcatcca ccagattccc
     6721 gttggaatct gtcggacgat catcacgagc aataaacccg ccggcaacgc ccgcagcagc
     6781 ataccggcga cccctcggcc tcgctgttcg ggctccacga aaacgccgga cagatgcgcc
     6841 ttgtgagcgt ccttggggcc gtcctcctgt ttgaagaccg acagcccaat gatctcgccg
     6901 tcgatgtagg cgccgaatgc cacggcatct cgcaaccgtt cagcgaacgc ctccatgggc
     6961 tttttctcct cgtgctcgta aacggacccg aacatctctg gagctttctt cagggccgac
     7021 aatcggatct cgcggaaatc ctgcacgtcg gccgctccaa gccgtcgaat ctgagcctta
     7081 atcacaattg tcaattttaa tcctctgttt atcggcagtt cgtagagcgc gccgtgcgcc
     7141 cgagcgatac tgagcgaagc aagtgcgtcg agcagtgccc gcttgttcct gaaatgccag
     7201 taaagcgctg gctgctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca
     7261 atgcaccagg tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc
     7321 gcaggcttcg ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat
     7381 gaggcggaag gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa
     7441 catccgtcgg gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg
     7501 gtcgccagca aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt
     7561 cttgccacgg tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac
     7621 gcggtcggac gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt
     7681 cgtgtaatac cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa
     7741 ggtgatcggc tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag
     7801 ttcgtcatcg tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac
     7861 gtggaaaatg accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa
     7921 cagggcagag cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat
     7981 atcgaacaag gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc
     8041 ctgcttggcc tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt
     8101 cgtcatagtt cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc
     8161 gagacgacgc gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg
     8221 cacgctgtcg cgctcgatct tggccgtagc ttgctggacc atcgagccga cggactggaa
     8281 ggtttcgcgg ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga
     8341 aaaccccgcg tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc gattcattca
     8401 ccctccttgc gggattgccc cgactcacgc cggggcaatg tgcccttatt cctgatttga
     8461 cccgcctggt gccttggtgt ccagataatc caccttatcg gcaatgaagt cggtcccgta
     8521 gaccgtctgg ccgtccttct cgtacttggt attccgaatc ttgccctgca cgaataccag
     8581 cgaccccttg cccaaatact tgccgtgggc ctcggcctga gagccaaaac acttgatgcg
     8641 gaagaagtcg gtgcgctcct gcttgtcgcc ggcatcgttg cgccactctt cattaaccgc
     8701 tatatcgaaa attgcttgcg gcttgttaga attgccatga cgtacctcgg tgtcacgggt
     8761 aagattaccg ataaactgga actgattatg gctcatatcg aaagtctcct tgagaaagga
     8821 gactctagtt tagctaaaca ttggttccgc tgtcaagaac tttagcggct aaaattttgc
     8881 gggccgcgac caaaggtgcg aggggcggct tccgctgtgt acaaccagat atttttcacc
     8941 aacatccttc gtctgctcga tgagcggggc atgacgaaac atgagctgtc ggagagggca
     9001 ggggtttcaa tttcgttttt atcagactta accaacggta aggccaaccc ctcgttgaag
     9061 gtgatggagg ccattgccga cgccctggaa actcccctac ctcttctcct ggagtccacc
     9121 gaccttgacc gcgaggcact cgcggagatt gcgggtcatc ctttcaagag cagcgtgccg
     9181 cccggatacg aacgcatcag tgtggttttg ccgtcacata aggcgtttat cgtaaagaaa
     9241 tggggcgacg acacccgaaa aaagctgcgt ggaaggctct gacgccaagg gttagggctt
     9301 gcacttcctt ctttagccgc taaaacggcc ccttctctgc gggccgtcgg ctcgcgcatc
     9361 atatcgacat cctcaacgga agccgtgccg cgaatggcat cgggcgggtg cgctttgaca
     9421 gttgttttct atcagaaccc ctacgtcgtg cggttcgatt agctgtttgt cttgcaggct
     9481 aaacactttc ggtatatcgt ttgcctgtgc gataatgttg ctaatgattt gttgcgtagg
     9541 ggttactgaa aagtgagcgg gaaagaagag tttcagacca tcaaggagcg ggccaagcgc
     9601 aagctggaac gcgacatggg tgcggacctg ttggccgcgc tcaacgaccc gaaaaccgtt
     9661 gaagtcatgc tcaacgcgga cggcaaggtg tggcacgaac gccttggcga gccgatgcgg
     9721 tacatctgcg acatgcggcc cagccagtcg caggcgatta tagaaacggt ggccggattc
     9781 cacggcaaag aggtcacgcg gcattcgccc atcctggaag gcgagttccc cttggatggc
     9841 agccgctttg ccggccaatt gccgccggtc gtggccgcgc caacctttgc gatccgcaag
     9901 cgcgcggtcg ccatcttcac gctggaacag tacgtcgagg cgggcatcat gacccgcgag
     9961 caatacgagg tcattaaaag cgccgtgatt gatgatatag cggcccggct gctcctggtt
    10021 ctcgcgcacc gaaatgggtg acttcacccc gcgctctttg atcgtggcac cgatttccgc
    10081 gatgctctcc ggggaaaagc cggggttgtc ggccgtccgc ggctgatgcg gatcttcgtc
    10141 gatcaggtcc aggtccagct cgatagggcc ggaaccgccc tgagacgccg caggagcgtc
    10201 caggaggctc gacaggtcgc cgatgctatc caaccccagg ccggacggct gcgccgcgcc
    10261 tgcggcttcc tgagcggccg cagcggtgtt tttcttggtg gtcttggctt gagccgcagt
    10321 cattgggaaa tctccatctt cgtgaacacg taatcagcca gggcgcgaac ctctttcgat
    10381 gccttgcgcg cggccgtttt cttgatcttc cagaccggca caccggatgc gagggcatcg
    10441 gcgatgctgc tgcgcaggcc aacggtggcc ggaatcatca tcttggggta cgcggccagc
    10501 agctcggctt ggtggcgcgc gtggcgcgga ttccgcgcat cgaccttgct gggcaccatg
    10561 ccaaggaatt gcagcttggc gttcttctgg cgcacgttcg caatggtcgt gaccatcttc
    10621 ttgatgccct ggatgctgta cgcctcaagc tcgatggggg acagcacata gtcggccgcg
    10681 aagagggcgg ccgccaggcc gacgccaagg gtcggggccg tgtcgatcag gcacacgtcg
    10741 aagccttggt tcgccagggc cttgatgttc gccccgaaca gctcgcgggc gtcgtccagc
    10801 gacagccgtt cggcgttcgc cagtaccggg ttggactcga tgagggcgag gcgcgcggcc
    10861 tggccgtcgc cggctgcggg tgcggtttcg gtccagccgc cggcagggac agcgccgaac
    10921 agcttgcttg catgcaggcc ggtagcaaag tccttgagcg tgtaggacgc attgccctgg
    10981 gggtccaggt cgatcacggc aacccgcaag ccgcgctcga aaaagtcgaa ggcaagatgc
    11041 acaagggtcg aagtcttgcc gacgccgcct ttctggttgg ccgtgaccaa agttttcatc
    11101 gtttggtttc ctgttttttc ttggcgtccg cttcccactt ccggacgatg tacgcctgat
    11161 gttccggcag aaccgccgtt acccgcgcgt acccctcggg caagttcttg tcctcgaacg
    11221 cggcccacac gcgatgcacc gcttgcgaca ctgcgcccct ggtcagtccc agcgacgttg
    11281 cgaacgtcgc ctgtggcttc ccatcgacta agacgccccg cgctatctcg atggtctgct
    11341 gccccacttc cagcccctgg atcgcctcct ggaactggct ttcggtaagc cgtttcttca
    11401 tggataacac ccataatttg ctccgcgcct tggttgaaca tagcggtgac agccgccagc
    11461 acatgagaga agtttagcta aacatttctc gcacgtcaac acctttagcc gctaaaactc
    11521 gtccttggcg taacaaaaca aaagcccgga aaccgggctt tcgtctcttg ccgcttatgg
    11581 ctctgcaccc ggctccatca ccaacaggtc gcgcacgcgc ttcactcggt tgcggatcga
    11641 cactgccagc ccaacaaagc cggttgccgc cgccgccagg atcgcgccga tgatgccggc
    11701 cacaccggcc atcgcccacc aggtcgccgc cctcactgcc cggcacctgg tcgctgaatg
    11761 tcgatgccag cacctgcggc acgtcaatgc ttccgggcgt cgcgctcggg ctgatcgccc
    11821 atcccgttac tgccccgatc ccggcaatgg caaggactgc cagcg
//
