pCPP5312 vector (V008217)

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LOCUS       40924_13155        6479 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCPP5312, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Kvitko BH, Ramos AR, Morello JE, Oh HS, Collmer A.
  TITLE     Identification of harpins in Pseudomonas syringae pv. tomato DC3000,
            which are functionally similar to HrpK1 in promoting translocation 
            of type III secretion system effectors
  JOURNAL   J. Bacteriol. 189 (22), 8059-8072 (2007)
  PUBMED    17873033
REFERENCE   2  (bases 1 to 6479)
  AUTHORS   Kvitko BH, Ramos AR, Morello JE, Oh H-S., Collmer A.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2007) Plant Pathology, Cornell University, 334 
            Plant Science, Ithaca, NY 14853, USA
REFERENCE   3  (bases 1 to 6479)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6479)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Bacteriol."; date: "2007"; volume: "189"; issue: "22"; pages: 
            "8059-8072"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-JUL-2007) Plant Pathology, Cornell University, 334 Plant 
            Science, Ithaca, NY 14853, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(15..43)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    210..234
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      353..385
                     /label=hrp box
                     /note="hrp box"
                     /regulatory_class="promoter"
     protein_bind    complement(928..952)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(2190..2297)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(2306..2324)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2334..2350)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3060..3719)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(3720..4489)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     misc_feature    complement(4714..5679)
                     /label=mob region
                     /note="mob region"
     CDS             complement(5775..6305)
                     /label=GmR
                     /note="gentamycin acetyltransferase"