LOCUS       40924_13155        6479 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCPP5312, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Kvitko BH, Ramos AR, Morello JE, Oh HS, Collmer A.
  TITLE     Identification of harpins in Pseudomonas syringae pv. tomato DC3000,
            which are functionally similar to HrpK1 in promoting translocation 
            of type III secretion system effectors
  JOURNAL   J. Bacteriol. 189 (22), 8059-8072 (2007)
  PUBMED    17873033
REFERENCE   2  (bases 1 to 6479)
  AUTHORS   Kvitko BH, Ramos AR, Morello JE, Oh H-S., Collmer A.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2007) Plant Pathology, Cornell University, 334 
            Plant Science, Ithaca, NY 14853, USA
REFERENCE   3  (bases 1 to 6479)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6479)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Bacteriol."; date: "2007"; volume: "189"; issue: "22"; pages: 
            "8059-8072"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-JUL-2007) Plant Pathology, Cornell University, 334 Plant 
            Science, Ithaca, NY 14853, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(15..43)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    210..234
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      353..385
                     /label=hrp box
                     /note="hrp box"
                     /regulatory_class="promoter"
     protein_bind    complement(928..952)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(2190..2297)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(2306..2324)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2334..2350)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3060..3719)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(3720..4489)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     misc_feature    complement(4714..5679)
                     /label=mob region
                     /note="mob region"
     CDS             complement(5775..6305)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
ORIGIN
        1 gctacttgca ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca
       61 tccgtttcca cggtgtgcgt ccatgggcaa atattatacg caaggcgaca aggtgctgat
      121 gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct
      181 taatgaatta caacagtttt tatgcatgca caagtttgta caaaaaagca ggctccgcgg
      241 ccgccccctt caccagccaa ggcagcgtta ctgtgcgcca aactcaagtc agtgacgctt
      301 tgatgtctaa acccggcgcc agcaaaattt tggggaatta tcttataagc ttggaaccga
      361 tccgctccct atgaccactc aagtggcgtc ataaatgctg tactaaagag ggtatacgaa
      421 tgggaaatat atgtgtcggc ggatccagga tggcccatca ggtgaactcc ccagaccgag
      481 ttagtaacaa ctcgggtgac gaagataacg taacgtccag tcaactgctg agcgtcagac
      541 atcaacttgc ggagtctgct ggtgtaccaa gagatcagca tgaatttgtt agtaaccaag
      601 cacctcaaag cctgagaaat cgctacaaca atctttactc acatacgcaa agaacactgg
      661 atatggcgga catgcagcat aggtacatga cgggagcgtc aggaatcaat ccgggaatgc
      721 tgccacatga gaatgtggac gatatgcgta gcgctataac tgattggagt gacatgcgcg
      781 aagctctgca gtacgcaatg ggtatccatg ccgacatccc accgtctcca gagcgatttg
      841 ttgcgactat gaacccgaac ggatcaattc gaatgtcaac actttctcct agcccgtacc
      901 gtaactggca aaagggtggg cgcgccgacc cagctttctt gtacaaagtg gttgatgggc
      961 agcaatcgca tcaggctggt tacgcaaacg ccgccgaccg ggagtctggc atccccgcag
     1021 ccgtactcga tggcatcaag gccgtggcga aggaaaaaaa cgccacattg atgttccgcc
     1081 tggtcaaccc ccattccacc agcctgattg ccgaaggggt ggccaccaaa ggattgggcg
     1141 tgcacgccaa gtcgtccgat tgggggttgc aggcgggcta cattcccgtc aacccgaatc
     1201 tttccaaact gttcggccgt gcgcccgagg tgatcgcgcg ggccgacaac gacgtcaaca
     1261 gcagcctggc gcatggccat accgcggtcg acctgacgct gtcgaaagag cggcttgact
     1321 atctgcggca agcgggcctg gtcaccggca tggccgatgg cgtggtcgcg agcaaccacg
     1381 caggctacga gcagttcgag tttcgcgtga aggaaacctc ggacgggcgc tatgccgtgc
     1441 agtatcgccg caagggcggc gacgatttcg aggcggtcaa ggtgatcggc aatgccgccg
     1501 gtattccact gacggcggat atcgacatgt tcgccattat gccgcatctg tccaacttcc
     1561 gcgactcggc gcgcagttcg gtgaccagcg gcgattcggt gaccgattac ctggcgcgca
     1621 cgcggcgggc cgccagcgag gccacgggcg gcctggatcg cgaacgcatc gacttgttgt
     1681 ggaaaatcgc tcgcgccggc gcccgttccg cagtgggcac cgaggcgcgt cgccagttcc
     1741 gctacgacgg cgacatgaat atcggcgtga tcaccgattt cgagctggaa gtgcgcaatg
     1801 cgctgaacag gcgggcgcac gccgtcggcg cgcaggacgt ggtccagcat ggcactgagc
     1861 agaacaatcc tttcccggag gcagatgaga agattttcgt cgtatcggcc accggtgaaa
     1921 gccagatgct cacgcgcggg caactgaagg aatacattgg ccagcagcgc ggcgagggct
     1981 atgtcttcta cgagaaccgt gcatacggcg tggcggggaa aagcctgttc gacgatgggc
     2041 tgggagccgc gcccggcgtg ccgagcggac gttcgaagtt ctcgccggat gtactggaaa
     2101 cggtgccggc gtcacccgga ttgcggcggc cgtcgctggg cgcagtggaa cgccaggatt
     2161 ccggctatga cagctaagaa ttcgatatcg gtaccgggcc ccccctcgag gtcgacggta
     2221 tcgataagct tgatatcgaa ttcctgcagc ccgggggatc cactagttct agagcggccg
     2281 ccaccgcggt ggagctccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     2341 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     2401 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     2461 caacagttgc gcagcctgaa tggcgaatgg aaattgtaag cgttaatatt ttgttaaaat
     2521 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgac tgcgatgagt
     2581 ggcagggcgg ggcgtaattt ttttaaggca gttattggtg cccttaaacg cctggtgcta
     2641 cgcctgaata agtgataata agcggatgaa tggcagaaat tcgaaagcaa attcgacccg
     2701 gtcgtcggtt cagggcaggg tcgttaaata gccgcttatg tctattgctg gtttaccggt
     2761 ttattgacta ccggaagcag tgtgaccgtg tgcttctcaa atgcctgagg ccagtttgct
     2821 caggctctcc ccgtggaggt aataattgac gatatgatca tttattctgc ctcccagagc
     2881 ctgataaaaa cggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg
     2941 cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgaggc
     3001 ggctacagcc gatagtctgg aacagcgcac ttacgggttg ctgcgcaacc caagtgctac
     3061 cggcgcggca gcgtgacccg tgtcggcggc tccaacggct cgccatcgtc cagaaaacac
     3121 ggctcatcgg gcatcggcag gcgctgctgc ccgcgccgtt cccattcctc cgtttcggtc
     3181 aaggctggca ggtctggttc catgcccgga atgccgggct ggctgggcgg ctcctcgccg
     3241 gggccggtcg gtagttgctg ctcgcccgga tacagggtcg ggatgcggcg caggtcgcca
     3301 tgccccaaca gcgattcgtc ctggtcgtcg tgatcaacca ccacggcggc actgaacacc
     3361 gacaggcgca actggtcgcg gggctggccc cacgccacgc ggtcattgac cacgtaggcc
     3421 gacacggtgc cggggccgtt gagcttcacg acggagatcc agcgctcggc caccaagtcc
     3481 ttgactgcgt attggaccgt ccgcaaagaa cgtccgatga gcttggaaag tgtcttctgg
     3541 ctgaccacca cggcgttctg gtggcccatc tgcgccacga ggtgatgcag cagcattgcc
     3601 gccgtgggtt tcctcgcaat aagcccggcc cacgcctcat gcgctttgcg ttccgtttgc
     3661 acccagtgac cgggcttgtt cttggcttga atgccgattt ctctggactg cgtggccatg
     3721 cttatctcca tgcggtaggg tgccgcacgg ttgcggcacc atgcgcaatc agctgcaact
     3781 tttcggcagc gcgacaacaa ttatgcgttg cgtaaaagtg gcagtcaatt acagattttc
     3841 tttaacctac gcaatgagct attgcggggg gtgccgcaat gagctgttgc gtacccccct
     3901 tttttaagtt gttgattttt aagtctttcg catttcgccc tatatctagt tctttggtgc
     3961 ccaaagaagg gcacccctgc ggggttcccc cacgccttcg gcgcggctcc ccctccggca
     4021 aaaagtggcc cctccggggc ttgttgatcg actgcgcggc cttcggcctt gcccaaggtg
     4081 gcgctgcccc cttggaaccc ccgcactcgc cgccgtgagg ctcggggggc aggcgggcgg
     4141 gcttcgcctt cgactgcccc cactcgcata ggcttgggtc gttccaggcg cgtcaaggcc
     4201 aagccgctgc gcggtcgctg cgcgagcctt gacccgcctt ccacttggtg tccaaccggc
     4261 aagcgaagcg cgcaggccgc aggccggagg cttttcccca gagaaaatta aaaaaattga
     4321 tggggcaagg ccgcaggccg cgcagttgga gccggtgggt atgtggtcga aggctgggta
     4381 gccggtgggc aatccctgtg gtcaagctcg tgggcaggcg cagcctgtcc atcagcttgt
     4441 ccagcagggt tgtccacggg ccgagcgaag cgagccagcc ggtggccgct cgcggccatc
     4501 gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga agcccggaga
     4561 gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa gcaaagtcta
     4621 gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct gcccccgtcg
     4681 agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc atgcgatgca
     4741 agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac cgcgagcgcg
     4801 agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg gccagcagca
     4861 ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc aaggacgctg
     4921 tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg gccagccaag
     4981 aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag tacggggcgg
     5041 atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg accgcgttcg
     5101 tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc aacaaagcgc
     5161 agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg ctgcaacggg
     5221 gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag gccctggagc
     5281 ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc gcctatgcac
     5341 cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg gaagccgtgg
     5401 ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag gccgccgcag
     5461 gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga gaccttcggg
     5521 agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg caggccaagg
     5581 ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg gaagtccagc
     5641 gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc gaaaagtgcc
     5701 acctggcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc gatctcggct
     5761 tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt
     5821 atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag
     5881 atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag cgcggtggca
     5941 atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc
     6001 ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg
     6061 aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc
     6121 ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca
     6181 gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt
     6241 gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctgcgt
     6301 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt
     6361 ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac
     6421 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatac
//
