Basic Vector Information
- Vector Name:
- pCPP5264
- Antibiotic Resistance:
- Tetracycline
- Length:
- 12904 bp
- Type:
- Cloning vector
- Replication origin:
- oriV
- Source/Author:
- Wei CF, Kvitko BH, Shimizu R, Crabill E, Alfano JR, Lin NC, Martin GB, Huang HC, Collmer A.
pCPP5264 vector Vector Map
pCPP5264 vector Sequence
LOCUS 40924_13150 12904 bp DNA circular SYN 17-DEC-2018
DEFINITION Cloning vector pCPP5264, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12904)
AUTHORS Wei CF, Kvitko BH, Shimizu R, Crabill E, Alfano JR, Lin NC, Martin
GB, Huang HC, Collmer A.
TITLE A Pseudomonas syringae pv. tomato DC3000 mutant lacking the type III
effector HopQ1-1 is able to cause disease in the model plant
Nicotiana benthamiana
JOURNAL Plant J. 51 (1), 32-46 (2007)
PUBMED 17559511
REFERENCE 2 (bases 1 to 12904)
AUTHORS Kvitko BH, Ramos AR, Morello JE, Oh HS, Collmer A.
TITLE Identification of harpins in Pseudomonas syringae pv. tomato DC3000,
which are functionally similar to HrpK1 in promoting translocation
of type III secretion system effectors
JOURNAL J. Bacteriol. 189 (22), 8059-8072 (2007)
PUBMED 17873033
REFERENCE 3 (bases 1 to 12904)
AUTHORS Kvitko BH, Ramos AR, Morello JE, Oh H-S., Collmer A.
TITLE Direct Submission
JOURNAL Submitted (09-JUL-2007) Plant Pathology, Cornell University, 334
Plant Science, Ithaca, NY 14853, USA
REFERENCE 4 (bases 1 to 12904)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 12904)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plant J.";
date: "2007"; volume: "51"; issue: "1"; pages: "32-46"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "J.
Bacteriol."; date: "2007"; volume: "189"; issue: "22"; pages:
"8059-8072"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(09-JUL-2007) Plant Pathology, Cornell University, 334 Plant
Science, Ithaca, NY 14853, USA"
COMMENT SGRef: number: 4; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..12904
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(65..81)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(89..105)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(113..143)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(158..179)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(414..782)
/label=traJ
/note="oriT-recognizing protein"
oriT complement(815..924)
/direction=LEFT
/label=oriT
/note="incP origin of transfer"
misc_feature 1056..1289
/note="similar to plasmid ColE1 in GenBank Accession Number
NC_001371"
misc_feature 1281..1433
/note="similar to plasmid ColE1 in GenBank Accession Number
NC_001371"
CDS complement(1608..2255)
/label=TetR
/note="tetracycline resistance regulatory protein"
CDS 2361..3557
/label=TcR
/note="tetracycline efflux protein"
CDS complement(4191..4856)
/codon_start=1
/product="unknown plasmid factor 16.5"
/label=unknown plasmid factor 16.5
/protein_id="ABS87292.1"
/translation="MTWCIANARALWGQFRLGVQQPALYWHFRNKRALLDALASLSIAR
AHGALYELPINRGLKLTIVIKAQIRRLGAADVQDFREIRLSALKKAPEMFGSVYEHEEK
KPMEAFAERLRDAVAFGAYIDGEIIGLSVFKQEDGPKDAHKAHLSGVFVEPEQRGRGVA
GMLLRALPAGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGSRRR"
CDS complement(4834..5979)
/label=trfA
/note="trans-acting replication protein that binds to and
activates oriV"
CDS complement(6028..6378)
/codon_start=1
/gene="ssb"
/product="single-stranded DNA binding protein"
/label=ssb
/protein_id="ABS87295.1"
/translation="MSHNQFQFIGNLTRDTEVRHGNSNKPQAIFDIAVNEEWRNDAGDK
QERTDFFRIKCFGSQAEAHGKYLGKGSLVFVQGKIRNTKYEKDGQTVYGTDFIADKVDY
LDTKAPGGSNQE"
gene complement(6028..6378)
/gene="ssb"
/label=ssb
CDS 6499..6864
/codon_start=1
/gene="trbA"
/product="TrbA"
/label=trbA
/protein_id="ABS87296.1"
/translation="MYNQIFFTNILRLLDERGMTKHELSERAGVSISFLSDLTNGKANP
SLKVMEAIADALETPLPLLLESTDLDREALAEIAGHPFKSSVPPGYERISVVLPSHKAF
IVKKWGDDTRKKLRGRL"
gene 6499..6864
/gene="trbA"
/label=trbA
CDS complement(7902..8996)
/codon_start=1
/gene="incC1"
/product="IncC1"
/label=incC1
/protein_id="ABS87297.1"
/translation="MGVIHEETAYRKPVPGGDPGAGSGAADHRDSAGRLSRWEATGDVR
NVAGTDQGRSVASGASRVGRVRGQELARGVRAGNGGSAGTSGVHRPEVGSGRQEKTGNQ
TMKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNASYTLKDFATGLHAS
KLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDARELFGANIKALAN
QGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMVTTIANVRQKNAKL
QFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADALASGVPVWKIKKT
AARKASKEVRALADYVFTKMEISQ"
gene complement(7902..8996)
/gene="incC1"
/label=incC1
CDS complement(7902..8681)
/codon_start=1
/gene="incC2"
/product="IncC2"
/label=incC2
/protein_id="ABS87298.1"
/translation="MKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNAS
YTLKDFATGLHASKLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDA
RELFGANIKALANQGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMV
TTIANVRQKNAKLQFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADA
LASGVPVWKIKKTAARKASKEVRALADYVFTKMEISQ"
gene complement(7902..8681)
/gene="incC2"
/label=incC2
CDS complement(8678..8983)
/codon_start=1
/gene="korA"
/product="KorA"
/label=korA
/protein_id="ABS87299.1"
/translation="MKKRLTESQFQEAIQGLEVGQQTIEIARGVLVDGKPQATFATSLG
LTRGAVSQAVHRVWAAFEDKNLPEGYARVTAVLPEHQAYIVRKWEADAKKKQETKR"
gene complement(8678..8983)
/gene="korA"
/label=korA
rep_origin complement(9448..10159)
/direction=LEFT
/label=oriV
/note="incP origin of replication"
misc_feature complement(10154..10307)
/note="similar to plasmid ColE1 in GenBank Accession Number
NC_001371"
misc_feature complement(10299..10529)
/note="similar to plasmid ColE1 in GenBank Accession Number
NC_001371"
primer_bind 10669..10685
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 10715..10739
/label=attB1
/note="recombination site for the Gateway(R) BP reaction"
RBS 10751..10771
/label=RBS
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS complement(10794..12062)
/label=FLP
/note="site-specific recombinase"
RBS 12066..12074
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
CDS 12163..12873
/label=lambda repressor (ts)
/note="temperature-sensitive variant of the phage lambda
repressor"
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