LOCUS       40924_13150       12904 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCPP5264, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12904)
  AUTHORS   Wei CF, Kvitko BH, Shimizu R, Crabill E, Alfano JR, Lin NC, Martin 
            GB, Huang HC, Collmer A.
  TITLE     A Pseudomonas syringae pv. tomato DC3000 mutant lacking the type III
            effector HopQ1-1 is able to cause disease in the model plant 
            Nicotiana benthamiana
  JOURNAL   Plant J. 51 (1), 32-46 (2007)
  PUBMED    17559511
REFERENCE   2  (bases 1 to 12904)
  AUTHORS   Kvitko BH, Ramos AR, Morello JE, Oh HS, Collmer A.
  TITLE     Identification of harpins in Pseudomonas syringae pv. tomato DC3000,
            which are functionally similar to HrpK1 in promoting translocation 
            of type III secretion system effectors
  JOURNAL   J. Bacteriol. 189 (22), 8059-8072 (2007)
  PUBMED    17873033
REFERENCE   3  (bases 1 to 12904)
  AUTHORS   Kvitko BH, Ramos AR, Morello JE, Oh H-S., Collmer A.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2007) Plant Pathology, Cornell University, 334 
            Plant Science, Ithaca, NY 14853, USA
REFERENCE   4  (bases 1 to 12904)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 12904)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2007"; volume: "51"; issue: "1"; pages: "32-46"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "J. 
            Bacteriol."; date: "2007"; volume: "189"; issue: "22"; pages: 
            "8059-8072"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (09-JUL-2007) Plant Pathology, Cornell University, 334 Plant 
            Science, Ithaca, NY 14853, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(65..81)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(89..105)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(113..143)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(158..179)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(414..782)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(815..924)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     misc_feature    1056..1289
                     /note="similar to plasmid ColE1 in GenBank Accession Number
                     NC_001371"
     misc_feature    1281..1433
                     /note="similar to plasmid ColE1 in GenBank Accession Number
                     NC_001371"
     CDS             complement(1608..2255)
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
     CDS             2361..3557
                     /label=TcR
                     /note="tetracycline efflux protein"
     CDS             complement(4191..4856)
                     /codon_start=1
                     /product="unknown plasmid factor 16.5"
                     /label=unknown plasmid factor 16.5
                     /protein_id="ABS87292.1"
                     /translation="MTWCIANARALWGQFRLGVQQPALYWHFRNKRALLDALASLSIAR
                     AHGALYELPINRGLKLTIVIKAQIRRLGAADVQDFREIRLSALKKAPEMFGSVYEHEEK
                     KPMEAFAERLRDAVAFGAYIDGEIIGLSVFKQEDGPKDAHKAHLSGVFVEPEQRGRGVA
                     GMLLRALPAGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGSRRR"
     CDS             complement(4834..5979)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     CDS             complement(6028..6378)
                     /codon_start=1
                     /gene="ssb"
                     /product="single-stranded DNA binding protein"
                     /label=ssb
                     /protein_id="ABS87295.1"
                     /translation="MSHNQFQFIGNLTRDTEVRHGNSNKPQAIFDIAVNEEWRNDAGDK
                     QERTDFFRIKCFGSQAEAHGKYLGKGSLVFVQGKIRNTKYEKDGQTVYGTDFIADKVDY
                     LDTKAPGGSNQE"
     gene            complement(6028..6378)
                     /gene="ssb"
                     /label=ssb
     CDS             6499..6864
                     /codon_start=1
                     /gene="trbA"
                     /product="TrbA"
                     /label=trbA
                     /protein_id="ABS87296.1"
                     /translation="MYNQIFFTNILRLLDERGMTKHELSERAGVSISFLSDLTNGKANP
                     SLKVMEAIADALETPLPLLLESTDLDREALAEIAGHPFKSSVPPGYERISVVLPSHKAF
                     IVKKWGDDTRKKLRGRL"
     gene            6499..6864
                     /gene="trbA"
                     /label=trbA
     CDS             complement(7902..8996)
                     /codon_start=1
                     /gene="incC1"
                     /product="IncC1"
                     /label=incC1
                     /protein_id="ABS87297.1"
                     /translation="MGVIHEETAYRKPVPGGDPGAGSGAADHRDSAGRLSRWEATGDVR
                     NVAGTDQGRSVASGASRVGRVRGQELARGVRAGNGGSAGTSGVHRPEVGSGRQEKTGNQ
                     TMKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNASYTLKDFATGLHAS
                     KLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDARELFGANIKALAN
                     QGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMVTTIANVRQKNAKL
                     QFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADALASGVPVWKIKKT
                     AARKASKEVRALADYVFTKMEISQ"
     gene            complement(7902..8996)
                     /gene="incC1"
                     /label=incC1
     CDS             complement(7902..8681)
                     /codon_start=1
                     /gene="incC2"
                     /product="IncC2"
                     /label=incC2
                     /protein_id="ABS87298.1"
                     /translation="MKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNAS
                     YTLKDFATGLHASKLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDA
                     RELFGANIKALANQGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMV
                     TTIANVRQKNAKLQFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADA
                     LASGVPVWKIKKTAARKASKEVRALADYVFTKMEISQ"
     gene            complement(7902..8681)
                     /gene="incC2"
                     /label=incC2
     CDS             complement(8678..8983)
                     /codon_start=1
                     /gene="korA"
                     /product="KorA"
                     /label=korA
                     /protein_id="ABS87299.1"
                     /translation="MKKRLTESQFQEAIQGLEVGQQTIEIARGVLVDGKPQATFATSLG
                     LTRGAVSQAVHRVWAAFEDKNLPEGYARVTAVLPEHQAYIVRKWEADAKKKQETKR"
     gene            complement(8678..8983)
                     /gene="korA"
                     /label=korA
     rep_origin      complement(9448..10159)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    complement(10154..10307)
                     /note="similar to plasmid ColE1 in GenBank Accession Number
                     NC_001371"
     misc_feature    complement(10299..10529)
                     /note="similar to plasmid ColE1 in GenBank Accession Number
                     NC_001371"
     primer_bind     10669..10685
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10715..10739
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             10751..10771
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(10794..12062)
                     /label=FLP
                     /note="site-specific recombinase"
     RBS             12066..12074
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             12163..12873
                     /label=lambda repressor (ts)
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
ORIGIN
        1 ttgtacaaag tggtgatgat cctctagagt cgacctgcag gcatgcaagc ttggcgtaat
       61 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
      121 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
      181 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
      241 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcggtc ttgccttgct
      301 cgtcggtgat gtacttcacc agctccgcga agtcgctctt cttgatggag cgcatgggga
      361 cgtgcttggc aatcacgcgc accccccggc cgttttagcg gctaaaaaag tcatggctct
      421 gccctcgggc ggaccacgcc catcatgacc ttgccaagct cgtcctgctt ctcttcgatc
      481 ttcgccagca gggcgaggat cgtggcatca ccgaaccgcg ccgtgcgcgg gtcgtcggtg
      541 agccagagtt tcagcaggcc gcccaggcgg cccaggtcgc cattgatgcg ggccagctcg
      601 cggacgtgct catagtccac gacgcccgtg attttgtagc cctggccgac ggccagcagg
      661 taggccgaca ggctcatgcc ggccgccgcc gccttttcct caatcgctct tcgttcgtct
      721 ggaaggcagt acaccttgat aggtgggctg cccttcctgg ttggcttggt ttcatcagcc
      781 atccgcttgc cctcatctgt tacgccggcg gtagccggcc agcctcgcag agcaggattc
      841 ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgcgggt
      901 gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca
      961 cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc
     1021 gctataatga ccccgaagca gggttatgca gcggaaaagc gccacgcttc ccgaagggag
     1081 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     1141 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     1201 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     1261 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     1321 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     1381 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gccagaaggc
     1441 cgccagagag gccgagcgcg gccgtgaggc ttggacgcta gggcagggca tgaaaaagcc
     1501 cgtagcgggc tgctacgggc gtctgacgcg gtggaaaggg ggaggggatg ttgtctacat
     1561 ggctctgctg tagtgagtgg gttgcgctcc ggcagcggtc ctgatcaatc gtcacccttt
     1621 ctcggtcctt caacgttcct gacaacgagc ctccttttcg ccaatccatc gacaatcacc
     1681 gcgagtccct gctcgaacgc tgcgtccgga ccggcttcgt cgaaggcgtc tatcgcggcc
     1741 cgcaacagcg gcgagagcgg agcctgttca acggtgccgc cgcgctcgcc ggcatcgctg
     1801 tcgccggcct gctcctcaag cacggcccca acagtgaagt agctgattgt catcagcgca
     1861 ttgacggcgt ccccggccga aaaacccgcc tcgcagagga agcgaagctg cgcgtcggcc
     1921 gtttccatct gcggtgcgcc cggtcgcgtg ccggcatgga tgcgcgcgcc atcgcggtag
     1981 gcgagcagcg cctgcctgaa gctgcgggca ttcccgatca gaaatgagcg ccagtcgtcg
     2041 tcggctctcg gcaccgaatg cgtatgattc tccgccagca tggcttcggc cagtgcgtcg
     2101 agcagcgccc gcttgttcct gaagtgccag taaagcgccg gctgctgaac ccccaaccgt
     2161 tccgccagtt tgcgtgtcgt cagaccgtct acgccgacct cgttcaacag gtccagggcg
     2221 gcacggatca ctgtattcgg ctgcaacttt gtcatgcttg acactttatc actgataaac
     2281 ataatatgtc caccaactta tcagtgataa agaatccgcg cgttcaatcg gaccagcgga
     2341 ggctggtccg gaggccagac atgaaaccca acatacccct gatcgtaatt ctgagcactg
     2401 tcgcgctcga cgctgtcggc atcggcctga ttatgccggt gctgccgggc ctcctgcgcg
     2461 atctggttca ctcgaacgac gtcaccgccc actatggcat tctgctggcg ctgtatgcgt
     2521 tggtgcaatt tgcctgcgca cctgtgctgg gcgcgctgtc ggatcgtttc gggcggcggc
     2581 caatcttgct cgtctcgctg gccggcgcca ctgtcgacta cgccatcatg gcgacagcgc
     2641 ctttcctttg ggttctctat atcgggcgga tcgtggccgg catcaccggg gcgactgggg
     2701 cggtagccgg cgcttatatt gccgatatca ctgatggcga tgagcgcgcg cggcacttcg
     2761 gcttcatgag cgcctgtttc gggttcggga tggtcgcggg acctgtgctc ggtgggctga
     2821 tgggcggttt ctccccccac gctccgttct tcgccgcggc agccttgaac ggcctcaatt
     2881 tcctgacggg ctgtttcctt ttgccggagt cgcacaaagg cgaacgccgg ccgttacgcc
     2941 gggaggctct caacccgctc gcttcgttcc ggtgggcccg gggcatgacc gtcgtcgccg
     3001 ccctgatggc ggtcttcttc atcatgcaac ttgtcggaca ggtgccggcc gcgctttggg
     3061 tcattttcgg cgaggatcgc tttcactggg acgcgaccac gatcggcatt tcgcttgccg
     3121 catttggcat tctgcattca ctcgcccagg caatgatcac cggccctgta gccgcccggc
     3181 tcggcgaaag gcgggcactc atgctcggaa tgattgccga cggcacaggc tacatcctgc
     3241 ttgccttcgc gacacgggga tggatggcgt tcccgatcat ggtcctgctt gcttcgggtg
     3301 gcatcggaat gccggcgctg caagcaatgt tgtccaggca ggtggatgag gaacgtcagg
     3361 ggcagctgca aggctcactg gcggcgctca ccagcctgac ctcgatcgtc ggacccctcc
     3421 tcttcacggc gatctatgcg gcttctataa caacgtggaa cgggtgggca tggattgcag
     3481 gcgctgccct ctacttgctc tgcctgccgg cgctgcgtcg cgggctttgg agcggcgcag
     3541 ggcaacgagc cgatcgctga tcgtggaaac gataggccta tgccatgcgg gtcaaggcga
     3601 cttccggcaa gctatacgcg ccctaggagt gcggttggaa cgttggccca gccagatact
     3661 cccgatcacg agcaggacgc cgatgatttg aagcgcactc agcgtctgat ccaagaacaa
     3721 ccatcctagc aacacggcgg tccccgggct gagaaagccc agtaaggaaa caactgtagg
     3781 ttcgagtcgc gagatccccc ggaaccaaag gaagtaggtt aaacccgctc cgatcaggcc
     3841 gagccacgcc aggccgagaa cattggttcc tgtaggcatc gggattggcg gatcaaacac
     3901 taaagctact ggaacgagca gaagtcctcc ggccgccagt tgccaggcgg taaaggtgag
     3961 cagaggcacg ggaggttgcc acttgcgggt cagcacggtt ccgaacgcca tggaaaccgc
     4021 ccccgccagg cccgctgcga cgccgacagg atctagcgct gcgtttggtg tcaacaccaa
     4081 cagcgccacg cccgcagttc cgcaaatagc ccccaggacc gccatcaatc gtatcgggct
     4141 acctagcaga gcggcagaga tgaacacgac catcagcggc tgcacagcgc ctaccgtcgc
     4201 cgcgacccgc ccggcaggcg gtagaccgaa ataaacaaca agctccagaa tagcgaaata
     4261 ttaagtgcgc cgaggatgaa gatgcgcatc caccagattc ccgttggaat ctgtcggacg
     4321 atcatcacga gcaataaacc cgccggcaac gcccgcagca gcataccggc gacccctcgg
     4381 cctcgctgtt cgggctccac gaaaacgccg gacagatgcg ccttgtgagc gtccttgggg
     4441 ccgtcctcct gtttgaagac cgacagccca atgatctcgc cgtcgatgta ggcgccgaat
     4501 gccacggcat ctcgcaaccg ttcagcgaac gcctccatgg gctttttctc ctcgtgctcg
     4561 taaacggacc cgaacatctc tggagctttc ttcagggccg acaatcggat ctcgcggaaa
     4621 tcctgcacgt cggccgctcc aagccgtcga atctgagcct taatcacaat tgtcaatttt
     4681 aatcctctgt ttatcggcag ttcgtagagc gcgccgtgcg cccgagcgat actgagcgaa
     4741 gcaagtgcgt cgagcagtgc ccgcttgttc ctgaaatgcc agtaaagcgc tggctgctga
     4801 acccccagcc ggaactgacc ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt
     4861 gacccaggcg tgttccacca ggccgctgcc tcgcaactct tcgcaggctt cgccgacctg
     4921 ctcgcgccac ttcttcacgc gggtggaatc cgatccgcac atgaggcgga aggtttccag
     4981 cttgagcggg tacggctccc ggtgcgagct gaaatagtcg aacatccgtc gggccgtcgg
     5041 cgacagcttg cggtacttct cccatatgaa tttcgtgtag tggtcgccag caaacagcac
     5101 gacgatttcc tcgtcgatca ggacctggca acgggacgtt ttcttgccac ggtccaggac
     5161 gcggaagcgg tgcagcagcg acaccgattc caggtgccca acgcggtcgg acgtgaagcc
     5221 catcgccgtc gcctgtaggc gcgacaggca ttcctcggcc ttcgtgtaat accggccatt
     5281 gatcgaccag cccaggtcct ggcaaagctc gtagaacgtg aaggtgatcg gctcgccgat
     5341 aggggtgcgc ttcgcgtact ccaacacctg ctgccacacc agttcgtcat cgtcggcccg
     5401 cagctcgacg ccggtgtagg tgatcttcac gtccttgttg acgtggaaaa tgaccttgtt
     5461 ttgcagcgcc tcgcgcggga ttttcttgtt gcgcgtggtg aacagggcag agcgggccgt
     5521 gtcgtttggc atcgctcgca tcgtgtccgg ccacggcgca atatcgaaca aggaaagctg
     5581 catttccttg atctgctgct tcgtgtgttt cagcaacgcg gcctgcttgg cctcgctgac
     5641 ctgttttgcc aggtcctcgc cggcggtttt tcgcttcttg gtcgtcatag ttcctcgcgt
     5701 gtcgatggtc atcgacttcg ccaaacctgc cgcctcctgt tcgagacgac gcgaacgctc
     5761 cacggcggcc gatggcgcgg gcagggcagg gggagccagt tgcacgctgt cgcgctcgat
     5821 cttggccgta gcttgctgga ccatcgagcc gacggactgg aaggtttcgc ggggcgcacg
     5881 catgacggtg cggcttgcga tggtttcggc atcctcggcg gaaaaccccg cgtcgatcag
     5941 ttcttgcctg tatgccttcc ggtcaaacgt ccgattcatt caccctcctt gcgggattgc
     6001 cccgactcac gccggggcaa tgtgccctta ttcctgattt gacccgcctg gtgccttggt
     6061 gtccagataa tccaccttat cggcaatgaa gtcggtcccg tagaccgtct ggccgtcctt
     6121 ctcgtacttg gtattccgaa tcttgccctg cacgaatacc agcgacccct tgcccaaata
     6181 cttgccgtgg gcctcggcct gagagccaaa acacttgatg cggaagaagt cggtgcgctc
     6241 ctgcttgtcg ccggcatcgt tgcgccactc ttcattaacc gctatatcga aaattgcttg
     6301 cggcttgtta gaattgccat gacgtacctc ggtgtcacgg gtaagattac cgataaactg
     6361 gaactgatta tggctcatat cgaaagtctc cttgagaaag gagactctag tttagctaaa
     6421 cattggttcc gctgtcaaga actttagcgg ctaaaatttt gcgggccgcg accaaaggtg
     6481 cgaggggcgg cttccgctgt gtacaaccag atatttttca ccaacatcct tcgtctgctc
     6541 gatgagcggg gcatgacgaa acatgagctg tcggagaggg caggggtttc aatttcgttt
     6601 ttatcagact taaccaacgg taaggccaac ccctcgttga aggtgatgga ggccattgcc
     6661 gacgccctgg aaactcccct acctcttctc ctggagtcca ccgaccttga ccgcgaggca
     6721 ctcgcggaga ttgcgggtca tcctttcaag agcagcgtgc cgcccggata cgaacgcatc
     6781 agtgtggttt tgccgtcaca taaggcgttt atcgtaaaga aatggggcga cgacacccga
     6841 aaaaagctgc gtggaaggct ctgacgccaa gggttagggc ttgcacttcc ttctttagcc
     6901 gctaaaacgg ccccttctct gcgggccgtc ggctcgcgca tcatatcgac atcctcaacg
     6961 gaagccgtgc cgcgaatggc atcgggcggg tgcgctttga cagttgtttt ctatcagaac
     7021 ccctacgtcg tgcggttcga ttagctgttt gtcttgcagg ctaaacactt tcggtatatc
     7081 gtttgcctgt gcgataatgt tgctaatgat ttgttgcgta ggggttactg aaaagtgagc
     7141 gggaaagaag agtttcagac catcaaggag cgggccaagc gcaagctgga acgcgacatg
     7201 ggtgcggacc tgttggccgc gctcaacgac ccgaaaaccg ttgaagtcat gctcaacgcg
     7261 gacggcaagg tgtggcacga acgccttggc gagccgatgc ggtacatctg cgacatgcgg
     7321 cccagccagt cgcaggcgat tatagaaacg gtggccggat tccacggcaa agaggtcacg
     7381 cggcattcgc ccatcctgga aggcgagttc cccttggatg gcagccgctt tgccggccaa
     7441 ttgccgccgg tcgtggccgc gccaaccttt gcgatccgca agcgcgcggt cgccatcttc
     7501 acgctggaac agtacgtcga ggcgggcatc atgacccgcg agcaatacga ggtcattaaa
     7561 agcgccgtga ttgatgatat agcggcccgg ctgctcctgg ttctcgcgca ccgaaatggg
     7621 tgacttcacc ccgcgctctt tgatcgtggc accgatttcc gcgatgctct ccggggaaaa
     7681 gccggggttg tcggccgtcc gcggctgatg cggatcttcg tcgatcaggt ccaggtccag
     7741 ctcgataggg ccggaaccgc cctgagacgc cgcaggagcg tccaggaggc tcgacaggtc
     7801 gccgatgcta tccaacccca ggccggacgg ctgcgccgcg cctgcggctt cctgagcggc
     7861 cgcagcggtg tttttcttgg tggtcttggc ttgagccgca gtcattggga aatctccatc
     7921 ttcgtgaaca cgtaatcagc cagggcgcga acctctttcg atgccttgcg cgcggccgtt
     7981 ttcttgatct tccagaccgg cacaccggat gcgagggcat cggcgatgct gctgcgcagg
     8041 ccaacggtgg ccggaatcat catcttgggg tacgcggcca gcagctcggc ttggtggcgc
     8101 gcgtggcgcg gattccgcgc atcgaccttg ctgggcacca tgccaaggaa ttgcagcttg
     8161 gcgttcttct ggcgcacgtt cgcaatggtc gtgaccatct tcttgatgcc ctggatgctg
     8221 tacgcctcaa gctcgatggg ggacagcaca tagtcggccg cgaagagggc ggccgccagg
     8281 ccgacgccaa gggtcggggc cgtgtcgatc aggcacacgt cgaagccttg gttcgccagg
     8341 gccttgatgt tcgccccgaa cagctcgcgg gcgtcgtcca gcgacagccg ttcggcgttc
     8401 gccagtaccg ggttggactc gatgagggcg aggcgcgcgg cctggccgtc gccggctgcg
     8461 ggtgcggttt cggtccagcc gccggcaggg acagcgccga acagcttgct tgcatgcagg
     8521 ccggtagcaa agtccttgag cgtgtaggac gcattgccct gggggtccag gtcgatcacg
     8581 gcaacccgca agccgcgctc gaaaaagtcg aaggcaagat gcacaagggt cgaagtcttg
     8641 ccgacgccgc ctttctggtt ggccgtgacc aaagttttca tcgtttggtt tcctgttttt
     8701 tcttggcgtc cgcttcccac ttccggacga tgtacgcctg atgttccggc agaaccgccg
     8761 ttacccgcgc gtacccctcg ggcaagttct tgtcctcgaa cgcggcccac acgcgatgca
     8821 ccgcttgcga cactgcgccc ctggtcagtc ccagcgacgt tgcgaacgtc gcctgtggct
     8881 tcccatcgac taagacgccc cgcgctatct cgatggtctg ctgccccact tccagcccct
     8941 ggatcgcctc ctggaactgg ctttcggtaa gccgtttctt catggataac acccataatt
     9001 tgctccgcgc cttggttgaa catagcggtg acagccgcca gcacatgaga gaagtttagc
     9061 taaacatttc tcgcacgtca acacctttag ccgctaaaac tcgtccttgg cgtaacaaaa
     9121 caaaagcccg gaaaccgggc tttcgtctct tgccgcttat ggctctgcac ccggctccat
     9181 caccaacagg tcgcgcacgc gcttcactcg gttgcggatc gacactgcca gcccaacaaa
     9241 gccggttgcc gccgccgcca ggatcgcgcc gatgatgccg gccacaccgg ccatcgccca
     9301 ccaggtcgcc gccctcactg cccggcacct ggtcgctgaa tgtcgatgcc agcacctgcg
     9361 gcacgtcaat gcttccgggc gtcgcgctcg ggctgatcgc ccatcccgtt actgccccga
     9421 tcccggcaat ggcaaggact gccagcgctg ccatttttgg ggtgaggccg ttcgcggccg
     9481 aggggcgcag cccctggggg gatgggaggc ccgcgttagc gggccgggag ggttcgagaa
     9541 gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa
     9601 acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg gtggccgaaa
     9661 aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc ctcaaatgtc
     9721 aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct
     9781 catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat ccccaggctt
     9841 gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct
     9901 ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg
     9961 ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt
    10021 tccgcgaggt atccacaacg ccggcggccg cggtgtctcg cacacggctt cgacggcgtt
    10081 tctggcgcgt ttgcagggcc atagacggcc gccagcccag cggcgagggc aaccagcccg
    10141 gtgagcgtcg gaaaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    10201 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    10261 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    10321 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    10381 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    10441 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    10501 cgcctttctc ccttcgggaa gcgtggcgcc attcgccatt caggctgcgc aactgttggg
    10561 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
    10621 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
    10681 ccagtgaatt cgagctcggt acccggggat catcacaagt ttgtacaaaa aagcaggctc
    10741 cgcggccgcc ttgtttaact ttaagaagga gcccttcacc aaatgcgtac ttatatgcgt
    10801 ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc attccatgcg
    10861 gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg ccactcctca
    10921 attggattag tctcatcctt caatgctatc atttcctttg atattggatc atatgcatag
    10981 taccgagaaa ctagtgcgaa gtagtgatca ggtattgctg ttatctgatg agtatacgtt
    11041 gtcctggcca cggcagaagc acgcttatcg ctccaatttc ccacaacatt agtcaactcc
    11101 gttaggccct tcattgaaag aaatgaggtc atcaaatgtc ttccaatgtg agattttggg
    11161 ccatttttta tagcaaagat tgaataaggc gcatttttct tcaaagcttt attgtacgat
    11221 ctgactaagt tatcttttaa taattggtat tcctgtttat tgcttgaaga attgccggtc
    11281 ctatttactc gttttaggac tggttcagaa ttcctcaaaa attcatccaa atatacaagt
    11341 ggatcgatcc taccccttgc gctaaagaag tatatgtgcc tactaacgct tgtctttgtc
    11401 tctgtcacta aacactggat tattactccc agatacttat tttggactaa tttaaatgat
    11461 ttcggatcaa cgttcttaat atcgctgaat cttccacaat tgatgaaagt agctaggaag
    11521 aggaattggt ataaagtttt tgtttttgta aatctcgaag tatactcaaa cgaatttagt
    11581 attttctcag tgatctccca gatgctttca ccctcactta gaagtgcttt aagcattttt
    11641 ttactgtggc tatttccctt atctgcttct tccgatgatt cgaactgtaa ttgcaaacta
    11701 cttacaatat cagtgatatc agattgatgt ttttgtccat agtaaggaat aattgtaaat
    11761 tcccaagcag gaatcaattt ctttaatgag gcttccagaa ttgttgcttt ttgcgtcttg
    11821 tatttaaact ggagtgattt attgacaata tcgaaactca gcgaattgct tatgatagta
    11881 ttatagctca tgaatgtggc tctcttgatt gctgttccgt tatgtgtaat catccaacat
    11941 aaataggtta gttcagcagc acataatgct attttctcac ctgaaggtct ttcaaacctt
    12001 tccacaaact gacgaacaag caccttaggt ggtgttttac ataatatacc aaattgtggc
    12061 atacaacctc cttagtacat gcaaccatta tcaccgccag aggtaaaata gtcaacacgc
    12121 acggtgttag atatttatcc cttgcggtga tagatttaac gtatgagcac aaaaaagaaa
    12181 ccattaacac aagagcagct tgaggacgca cgtcgcctta aagcaattta tgaaaaaaag
    12241 aaaaatgaac ttggcttatc ccaggaatct gtcgcagaca agatggggat ggggcagtca
    12301 ggcgttggtg ctttatttaa tggcatcaat gcattaaatg cttataacgc cgcattgctt
    12361 acaaaaattc tcaaagttag cgttgaagaa tttagccctt caatcgccag agaaatctac
    12421 gagatgtatg aagcggttag tatgcagccg tcacttagaa gtgagtatga gtaccctgtt
    12481 ttttctcatg ttcaggcagg gatgttctca cctaagctta gaacctttac caaaggtgat
    12541 gcggagagat gggtaagcac aaccaaaaaa gccagtgatt ctgcattctg gcttgaggtt
    12601 gaaggtaatt ccatgaccgc accaacaggc tccaagccaa gctttcctga cggaatgtta
    12661 attctcgttg accctgagca ggctgttgag ccaggtgatt tctgcatagc cagacttggg
    12721 ggtgatgagt ttaccttcaa gaaactgatc agggatagcg gtcaggtgtt tttacaacca
    12781 ctaaacccac agtacccaat gatcccatgc aatgagagtt gttccgttgt ggggaaagtt
    12841 atcgctagtc agtggcctga agagacgttt ggctgataag ggtgggcgcg ccgacccagc
    12901 tttc
//
