pCPCbLCVA.007 vector (V008227)

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LOCUS       V008227                 5352 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008227
VERSION     V008227
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Turnage MA, Muangsan N, Peele CG, Robertson D.
  TITLE     Geminivirus-based vectors for gene silencing in Arabidopsis
  JOURNAL   Plant J. 30 (1), 107-114 (2002)
   PUBMED   11967097
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Turnage MA, Muangsan N, Peele CG, Robertson D.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-APR-2003) Botany, North Carolina State University, Box
            7612, Raleigh, NC 27695, USA
REFERENCE   3  (bases 1 to 5352)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5352)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J.";
            date: "2002"; volume: "30"; issue: "1"; pages: "107-114"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-APR-2003) Botany, North Carolina State University, Box 7612,
            Raleigh, NC 27695, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(695..1081)
                     /note="Transcriptional activator protein from Cabbage leaf
                     curl virus (isolate Jamaica). Accession#: Q96703"
                     /label="Transcriptional activator protein"
     CDS             complement(1026..2072)
                     /note="Replication-associated protein from Cabbage leaf
                     curl virus (isolate Jamaica). Accession#: Q96704"
                     /label="Replication-associated protein"
     promoter        complement(2507..2525)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2535..2551)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      2693..3148
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3174..3278
                     /label="AmpR promoter"
     CDS             3279..4136
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4310..4898
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    5186..5207
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5222..5252
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5260..5276
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5284..5300
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5321..5339
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"