LOCUS       V008227                 5352 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008227
VERSION     V008227
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Turnage MA, Muangsan N, Peele CG, Robertson D.
  TITLE     Geminivirus-based vectors for gene silencing in Arabidopsis
  JOURNAL   Plant J. 30 (1), 107-114 (2002)
   PUBMED   11967097
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Turnage MA, Muangsan N, Peele CG, Robertson D.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-APR-2003) Botany, North Carolina State University, Box
            7612, Raleigh, NC 27695, USA
REFERENCE   3  (bases 1 to 5352)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5352)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J.";
            date: "2002"; volume: "30"; issue: "1"; pages: "107-114"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-APR-2003) Botany, North Carolina State University, Box 7612,
            Raleigh, NC 27695, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(695..1081)
                     /note="Transcriptional activator protein from Cabbage leaf
                     curl virus (isolate Jamaica). Accession#: Q96703"
                     /label="Transcriptional activator protein"
     CDS             complement(1026..2072)
                     /note="Replication-associated protein from Cabbage leaf
                     curl virus (isolate Jamaica). Accession#: Q96704"
                     /label="Replication-associated protein"
     promoter        complement(2507..2525)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2535..2551)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      2693..3148
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3174..3278
                     /label="AmpR promoter"
     CDS             3279..4136
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4310..4898
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    5186..5207
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5222..5252
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5260..5276
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5284..5300
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5321..5339
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agctccaccg cggtggcggc cgctctagtg gatcccccgc ttcatctttg ggtatgtcgc
       61 accttaggtg agttaagaat atatttcggg ctgctaaacg aaacgattta gggtttcgtg
      121 gcatttttgt aaataatatt taggactcca ggggaggacg ccaggagagc tctctctaaa
      181 acctattgtt tttggtgtct tggtgtccaa tatatactaa aagcctctgg ggaacaccag
      241 gggcaaaagc ggccatccgc aataatatta ccggatggcc gcgatttttt tggtgtcttg
      301 cgtgggacca cgcattaaat gaaatctaac caatcacatg ccgcctgaca aggttagcta
      361 ttaaggctaa ctgagtgcgc tgtgggccta tataaagaca tgtgatttca atttatgctt
      421 tacttcgaaa tgcctaagcg gctagcggta ccctcgagtc tagaggatcc agatctgagc
      481 tcgctagcct aatcctgtgt atgcgacatt aaaaattcgg atctattttt acgattcgat
      541 aacaaattaa taaattttga aatttattac atgattctcg tgaacatgag ttacatagga
      601 tctgtccgtt gcgaaacgaa cagctctaat tacatgatta ataccaataa cacctaggtt
      661 atctaaatga gacattacaa ggcatttgaa tctacttaaa tatgtcggcc cagaagctct
      721 catcgaagtc gtccagactt ggaagttgaa gtaggctttg cggagatcca atgctttccg
      781 taggttgtgg ttgaaccgga cttggatgtg gtagatcctg gttctggtgt acaacgggtc
      841 ctctacgcgt tgtatcctga aatatagggg atttggaacc tcccagataa aaacggaatt
      901 ctctgcctga gctacagtga tgctctcccc ggtgcgtgaa tccattatct gcgcaattga
      961 tgtggaggaa gatagaacac ccgcagttca aatcaatgcg tcttcggcgt acagctctcc
     1021 ttttagcaat cttgtgctgt gctttgatag aggggggctt caagagtgat gaattttgca
     1081 tttttggtag tccacgctct tagtgatgca ttttcctctt tgttgaggaa acttatataa
     1141 ctgctcccct ctcctggatt gcacagcacg attgagggta tgccaccttt aatttgaact
     1201 ggcttgccgt acttacagtt tgattgccag tccctttggg ccccaataag ctctttccag
     1261 tgctttagct ttagataatg cggagctatg tcatcaatga cgttatactc cgcattattt
     1321 gaaaagacct ttgaattaaa gtcgaggtgc ccactcaaat aattatgtgg tcctaaagca
     1381 cgcgcccaca tggtcttgcc ggttcgtgaa tcaccttcaa ctatgatact aataggtctt
     1441 tccgcccgcg cagcggcact ccgaccaaaa tagtcatctg cccatgaact catctcgtcc
     1501 gggacgttag taaaggagga gagttgaaac ggaggagccc atggttccgg agccttagta
     1561 aagagtcgct ctatgttagc tctaacatta tgataactaa caatgaacgt ctttggaact
     1621 ccagccctta taattgcgag acgctcttcc acacatcccg cattgacggc gttgtggtag
     1681 acgtcgtctt tatttgcctt tgtaccccca gacaccttgt actgcccgga ttcacaataa
     1741 tcaccatctt tggtgatgta attcttgacg gcattggtgt ctttggctgc ctgaatgttt
     1801 gggtgaaaat tggcagacct tctggggtga gtgatgtcga aaaatctagc atccttgatg
     1861 ttcgactttc ctgatagttg gatgagacag tgtgtaaatg gggaaccgtc tgaatgttcc
     1921 tctcttgcga ctctgatgta tgtgggtttg acgactgacc acgacagggt ttgaagcatc
     1981 tgaagagctt catctttggg tatgtcgcac cttaggtgag ttaagaatat atttcgggct
     2041 gctaaacgaa acgatttagg gtttcgtggc atttttgtaa ataatattta ggactccagg
     2101 ggaggacgcc aggagagctc tctctaaaac ctattgtttt tggtgtcttg gtgtccaata
     2161 tatactaaaa gcctctgggg aacaccaggg gcaaaagcgg ccatccgcaa taatattacc
     2221 ggatggccgc gatttttttg gtgtcttgcg tgggaccacg cattaaatga aatctaacca
     2281 atcacatgcc gcctgacaag gttagctatt aaggctaact gagtgcgctg tgggcctata
     2341 taaagacatg tgatttcaat ttatgcttta cttcgaaatg cctaagcggg atgccccgtg
     2401 gcgttctatg gcggggacct ctaaagtgtc ccgcaatgct aactattcac ctcgtgcagg
     2461 tatgatccat aaatttgata aggccgctgc ttgggttcca attcgcccta tagtgagtcg
     2521 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     2581 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     2641 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     2701 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     2761 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     2821 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     2881 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     2941 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3001 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3061 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3121 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     3181 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     3241 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     3301 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     3361 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     3421 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     3481 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     3541 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     3601 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     3661 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     3721 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     3781 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     3841 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     3901 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     3961 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4021 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4081 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4141 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     4201 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     4261 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     4321 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     4381 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4441 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4501 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4561 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4621 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4681 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4741 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4801 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4861 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4921 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     4981 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     5041 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     5101 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     5161 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     5221 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     5281 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     5341 aacaaaagct gg
//
