pCPC77 vector (V008233)

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LOCUS       V008233                 6997 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008233
VERSION     V008233
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6997)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6997)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6997)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    201..471
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     CDS             905..931
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             935..961
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             965..991
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     rep_origin      complement(1630..2218)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2392..3249)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3250..3354)
                     /label="AmpR promoter"
     regulatory      3425..4159
                     /note="promoter region of ADH1 gene of Candida albicans;
                     CaADH1p"
                     /regulatory_class="promoter"
     CDS             4160..4777
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     CDS             4799..5140
                     /label="GAL4 activation domain"
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     regulatory      5158..5464
                     /note="terminator region of ACT1 gene of Candida albicans;
                     ACT1 terminator"
                     /regulatory_class="terminator"
     CDS             5926..6819
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"