LOCUS       V008233                 6997 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008233
VERSION     V008233
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6997)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6997)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6997)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    201..471
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     CDS             905..931
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             935..961
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             965..991
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     rep_origin      complement(1630..2218)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2392..3249)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3250..3354)
                     /label="AmpR promoter"
     regulatory      3425..4159
                     /note="promoter region of ADH1 gene of Candida albicans;
                     CaADH1p"
                     /regulatory_class="promoter"
     CDS             4160..4777
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     CDS             4799..5140
                     /label="GAL4 activation domain"
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     regulatory      5158..5464
                     /note="terminator region of ACT1 gene of Candida albicans;
                     ACT1 terminator"
                     /regulatory_class="terminator"
     CDS             5926..6819
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
ORIGIN
        1 ttcgaccgtc atgtcctttc tataatgttt atctaacaaa gatgttgtgt aaaatgcagc
       61 gtagccatga gcaccatcag tcagtcagtc aggcttggac agcatcagca gtagcgtatt
      121 gttcgaatga accatcgtga gtgtaaccag acaagtcagc ttcaccacag tttggttcag
      181 caccttgtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
      241 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
      301 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
      361 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
      421 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
      481 gtcgagctcc aaacttcttt tttatttttc gcaatattgt cgataaatta aatataaact
      541 actcgattga gtaatagatt caataactat gggaattact aacaatgatt taaggaaaaa
      601 gcttcactta aaaataaagc cctttcaatt aaagtgttgt ccatgtttgt cgtttctgat
      661 gggcttttct gaaatataaa tatagattca aatctcttgt attcaagttt tagatttttt
      721 tttatttctt ttatatcatt atcaaatcaa atcaacaaac tctttcaact tctcagaaag
      781 aaatcaatat caactacaat aaaaccaact acgtctacta ctacgttaac tcctacacac
      841 atacaaatat aaatagttat tcatgtccac acgcaagctc gcgagcgcgc caattagatc
      901 cgtgtaccca tacgatgttc cagattacgc tggttaccca tacgatgttc cagattacgc
      961 tggttaccca tacgatgttc cagattacgc tatcgatatc ccatggagat ctacgctagg
     1021 atcgatatct cgagacgcgt gatcttggac accagtaaat aagcaaatag ctaaatcata
     1081 tacgaattaa tgttatgatt aagtgtttac gtgagtgcga tatttttatt actatcttat
     1141 acagttgtat atactctata aaatgagttg tctattaatt aacgatgaat gctttctggg
     1201 tttacctctc caacgactct agtttacttc tcaatacatt caattgtatt tgatttgtca
     1261 atacttcatc attaatcaat tctatagttt tgtttttctc gtttatttcc aaatttaatg
     1321 catcaatttt attattcaat ttgtcgttga ttttggttaa tgattttatg gtttgatctc
     1381 tggcattgat tgtttgtgtt agtttttcat tattgataat taaactattt aagttagtta
     1441 tcaactcggt gttttcaagt tttaagtttt caatttcttt agagtttata agatttgtca
     1501 aagtttctga attgcttgat tggtcctgta gaagcgtgtc taagaactcg aggataacgc
     1561 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1621 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1681 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1741 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1801 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1861 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1921 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1981 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2041 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     2101 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     2161 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     2221 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     2281 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     2341 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     2401 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     2461 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     2521 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     2581 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     2641 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2701 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     2761 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     2821 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     2881 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     2941 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     3001 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     3061 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     3121 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     3181 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     3241 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3301 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     3361 tccccgaaaa gtgccacctg acgtctaaga accgcggagg cctgggcccc cgggtaccga
     3421 gctcgccagc gagaaagagg agtattggca ttgttgggta gtagatgaat gagtaatctt
     3481 ctagccaaaa caaaataaac cacaagaaca gtaggcaaaa ctgagtactt gatagcgctt
     3541 gtagtctttg tatttaaaaa aacctaattg tccaaaaatt gaaaatcatg agttgcaaat
     3601 ttgttgaaat caccaggtaa atagatcatt tagctatatc gccttatcat tcttcgtaca
     3661 agaattatac acaaatacca ccaccacacc acccagaaat tttacacgtg ttttctaaat
     3721 tattactttc cgtggccaat caaattgaaa tttttcttgt tcgaaacggc agtgcctcaa
     3781 acattatagt agcaagtatt gcctaaagag tttccgtctc gtgccccccc cctatcccct
     3841 tttgtaattt gccaaaatag ctcaaaaagg ataatcccgc actttttttt tccgagttct
     3901 cgtcatgcaa gcaagcttat tcagaatttt cagaggtgcg agtctcacca ctaccactgc
     3961 agctgcatct tttacagcaa cagcaacagc aggtgccacc acggcaaaga cattgtctgg
     4021 atccactgtg ctccgaaaac tgtataaaag gacctattca tccctggtct tatcttctcc
     4081 agaattattt ttttttcatc agtttaacaa caacaaacgt tattgtcata caacaacaac
     4141 aacaaataca aaaacaatta tgtcaagatt agataaatca aaagttatta attcagcttt
     4201 agaattatta aatggtgttg gtattgaagg tttaactact agaaaattag ctcaaaaatt
     4261 aggtgttgaa caaccaactt tatattggca tgttaaaaat aaaagagctt tattagatgc
     4321 tttaccaatt gaaatgttag atagacatca tactcattca tgtccattag aaggtgaatc
     4381 atggcaagat tttttaagaa ataatgctaa atcatataga tgtgctttat tatcacatag
     4441 agatggtgct aaagttcatt taggtactag accaactgaa aaacaatatg aaactttaga
     4501 aaatcaatta gcttttttat gtcaacaagg tttttcatta gaaaatgctt tatatgcttt
     4561 atcagctgtt ggtcatttta ctttaggttg tgttttagaa gaacaagaac atcaagttgc
     4621 taaagaagaa agagaaactc caactactga ttcaatgcca ccattattaa aacaagctat
     4681 tgaattattt gatagacaag gtgctgaacc agctttttta tttggtttag aattaattat
     4741 ttgtggttta gaaaaacaat taaaatgtga atcaggtggt tcgactttgt ttggtggcgc
     4801 caattttaat caaagtggga atattgctga tagctcattg tccttcactt tcactaacag
     4861 tagcaacggt ccgaacctca taacaactca aacaaattct caagcgcttt cacaaccaat
     4921 tgcctcctct aacgttcatg ataacttcat gaataatgaa atcacggcta gtaaaattga
     4981 tgatggtaat aattcaaaac cattgtcacc tggttggacg gaccaaactg cgtataacgc
     5041 gtttggaatc actacaggga tgtttaatac cactacaatg gatgatgtat ataactatct
     5101 attcgatgat gaagataccc caccaaaccc aaaaaaagag taaaccgtgc atcgctacca
     5161 ttgttcacca caaatgtttc taagagtgaa attctggaaa tctggaaatc tggttttgta
     5221 ttcttgttat tcttcttttt gttattacat atataacttg ttactttttt aaaaaaatct
     5281 ttgtttattt tataaatata taaaactaaa tttaagaaaa agagaaaaat gttttatttg
     5341 agagattgaa attttacttg aatttagctt agcttttata aagtattatt atgtaaaaaa
     5401 acaaaacaaa tatacattaa aaagttaaga ctataaaata gccacccaag gcatttctat
     5461 atctacagtc tgccagactt gtatggtaat ccaaatgggt atggggttag tagtaatttg
     5521 gtagtgaaaa ccccggtaga cggagaatta ataaataccc caaaatccac cacaggactc
     5581 gacaggtacc tggaggatga ggagacagaa gttagtagta acaatttggg gacagaagag
     5641 gatacagtga catcagagga agaagacagc gaaggtatag tcaaaaagca ataggacagc
     5701 aagacaattt atttatttaa tagtttttta taaatgtaaa atctatcaca acatgtgtac
     5761 ttaattttct gtggtagggg aggccatttc gttgaaacgt gtcgcgagcg gttgtgagaa
     5821 ttgactcatt ttgattatta cgcaaccatt gaaatttttt attttttttt ggtgaagatt
     5881 tttcccacac aacttcttct tttacttaac caccaactac cgataatgga tttagtcaat
     5941 catttaccag accgtttgtt atttgctgtt cctaaaaagg gcagattata cgaaaaatgc
     6001 tgtaacttat tgagtggtgc cgatatacag tttagaagat ctaatagatt agatatagca
     6061 ctttctacaa acttgccaat tgcattaatc ttcttgcctg cagctgatat cccagttttc
     6121 gttggagaag gtaattgtga cttgggtata actgggttag accaaatcaa agaagctgaa
     6181 caattcgaca acatcgagga cttgttggat ttgaaatttg gttcatgcaa attgcagatc
     6241 caagttccag cagatggcga gtacgaaaag ccagaacagc ttgttggaaa gaaaattgtg
     6301 tcttcattta caaaattgag taccgactat ttcaaacaat tgtcagacaa acctactaat
     6361 atcagatatg tcggtggttc cgttgaggct tcttgtgcct tgggtgttgc tgatgctatt
     6421 gtcgatttgg ttgaaagtgg tgaaactatg aaagcagctg ggttaaaggc catagaaacc
     6481 atattggaga cttcggctca tttgatttcg tcaaagaaaa gtaaattccc agaaatggtc
     6541 aatataatcg tccaaagact tcaaggtgtt ttagctgccc aggaatatgt cttgtgtaac
     6601 tacaatgccc caaaatccat ccaagcaaaa tgtttaacca ttactccagg cagaagggcc
     6661 gctactgtct ctactttgga caaacacagc gacgacgaag aggactgggt tgccatctca
     6721 tccatggtta atagaaagga aatcggtaat gtaatggacg aattgaagaa agctggtgca
     6781 accgatatat tggtactcga gatatcaaat tgtagagttt aaaagaagtg atagtttctc
     6841 ataaatatta tacatatata cttatagatc tacttcaatt tgactcaatc tgtcttatac
     6901 catctttatc taccaccttg atgtacacac ctttaaagtc aataggcatt ctcgtttgta
     6961 attccttgac acacatatcc attaacttta aaccatc
//
