Basic Vector Information
- Vector Name:
- pCPC48
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4203 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Chang P, Wang W, Chen J.
pCPC48 vector Map
pCPC48 vector Sequence
LOCUS V008247 4203 bp DNA circular SYN 17-DEC-2018
DEFINITION Exported.
ACCESSION V008247
VERSION V008247
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 4203)
AUTHORS Chang P, Wang W, Chen J.
TITLE Fast and convenient doxycycline-induced marker recycling in Candida
albicans via Cre-loxP system
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4203)
AUTHORS Chang P, Wang W, Chen J.
TITLE Direct Submission
REFERENCE 3 (bases 1 to 4203)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4203)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(30-JAN-2018) Research Center of Bioenergy "
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4203
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter complement(476..580)
/label="AmpR promoter"
CDS 1549..2667
/gene="LEU2"
/label="3-isopropylmalate dehydrogenase"
/note="3-isopropylmalate dehydrogenase from Candida
maltosa. Accession#: P07139"
protein_bind complement(2920..2953)
/label="loxP"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
rep_origin complement(3059..3647)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(join(3821..4203,1..475))
/label="AmpR"
/note="beta-lactamase"
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