LOCUS       V008247                 4203 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008247
VERSION     V008247
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Fast and convenient doxycycline-induced marker recycling in Candida
            albicans via Cre-loxP system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4203)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4203)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(476..580)
                     /label="AmpR promoter"
     CDS             1549..2667
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     protein_bind    complement(2920..2953)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(3059..3647)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(join(3821..4203,1..475))
                     /label="AmpR"
                     /note="beta-lactamase"
ORIGIN
        1 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
       61 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
      121 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
      181 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
      241 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
      301 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
      361 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
      421 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
      481 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
      541 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
      601 cacctgacgt ctaagaaaat aacttcgtat agcatacatt atacgaagtt atagctcgga
      661 tccactagta acggccgcca gtgtgctgga attcgccctt ggatccaatc atcactggtg
      721 ggggtcaaga aagaggtttg agatcaggta ctttggcacc accattagta gctgggtttg
      781 gtgaagctgc aagattaatg aaacaagaat ctgcatttga caagaaacat attgagaggt
      841 tatctactaa attgaagaac ggtttgttat ccattccatc tactcagttt aacggttgta
      901 ataacccaac atatcaatac ccaggttgtg tgaatgtttc ctttgcatac attgaagggg
      961 aatctttgtt aatggcattg aaagatattg cattgagttc tggttctgca tgtacctctg
     1021 catctttgga accatcgtat gttttacatg ctttgggtgc cgatgatgcc ttggctcatt
     1081 cttccattag attcggtatt ggtagattca ccactgaagc agaagttgac tatgttattc
     1141 aagcaattaa tgaaagagtt gatttcttga gaaagatgtc tccattatgg gaaatggttc
     1201 aagaaggtat tgacttgaac tctatcgaat ggagtggaca ttaaggtttt ttgttttttt
     1261 gtaatttgtg ttgttatgtg taattatcgt gtatagagta atttcggaaa ttgtactttc
     1321 gttcatgtat ctgaaccaga tgaataatac gttcgtattg ttgaagaatg actaactcct
     1381 gtaacggcgt gcgtgggttc cgcagccggg ttcaattttt tttttcttgt tcactataaa
     1441 aatatataat aaacgaaaaa aatccgaggg actcaggcta tgaaattttt cactcaagaa
     1501 ttttttttcc ttcatcctta attcctttat tcactaccca aacccacaat gtcagttaaa
     1561 actaaaacta ttacaatttt accaggtgat cacgtcggta ccgaaattgt caatgaagct
     1621 atcaaagttt tagaagcaat tgaagctgcc actccttacc aaaaaatcca tttcgatttc
     1681 aaacatcatt taatcggtgg tgctgccatt gatgccactg gtgtcccatt gccagatgac
     1741 gcacttgaaa gtgccaaaaa atctgatgcc gtcttattgg gtgctgttgg tggaccaaaa
     1801 tgggggactg gcgccgttcg tccagaacaa ggtttattaa agattcgtaa agaattgaac
     1861 ctttatgcta acatcagacc atgtaacttt gccagtgact ccttattgga attatcccca
     1921 ttgagaccag aagtcgttaa aggtacaaac ttgatcattg ttcgtgaatt agttggtggt
     1981 atttatttcg gtgatcgtga agaacaagaa gaaagtgaag ataaacaaac cgcatgggat
     2041 actgaaaaat acaccgtcga tgaagtcacc agaatcaccc gtatggctgc cttcatggct
     2101 ttacaacata acccaccatt accaatctgg tcattggata aggctaatgt tttagcttct
     2161 tctagattat ggagaagaac tgtcgataaa gtgatttctg aagagttccc aactttgtct
     2221 gttcaacatc aattgattga ctccgccgcc atgattttaa ttcaaaaccc aaccaaattg
     2281 aatggtataa tcatcacttc taacatgttt ggtgatatca tttccgatga agcttcagtt
     2341 attccaggtt ccttgggttt attaccatct gcatctttgg cttcattgcc agataccaac
     2401 accgcttttg gtctttatga accatgtcac ggttctgctc ctgatttacc agccaataaa
     2461 gttaacccaa ttgctactat tttatctgct gcttctatgt tgagattatc tttggattgt
     2521 gtgaaagaag ctgaagcttt agaagaagcc gtgaaacaag tcttggataa aggtatcaga
     2581 actgcagatt taagaggtac cagttcagct actgaagttg gtgacgcgat tgtcgaagct
     2641 gttactaaaa tcttaaaaga aaaagcttaa gtaaagtata cacaaaattt aacgaatatt
     2701 attaatacaa aatgaacacc acctaaatac ttttttttaa aaatgtaccg actcttctaa
     2761 aaattgcgat aattcaatat cggaaatttc tctcaccggt ttacttggat cttcggaaat
     2821 aaatctattt gaagtaatgg tagcctcact tgattctgat tggctctttc tagacatggg
     2881 taggtaaggg cgaattctgc agatatccat cacactggta taacttcgta tagcatacat
     2941 tatacgaagt tatggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     3001 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     3061 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     3121 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     3181 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     3241 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     3301 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3361 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3421 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3481 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3541 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3601 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3661 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3721 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3781 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3841 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3901 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3961 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     4021 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     4081 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     4141 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     4201 ggc
//
