pCPC27 vector (V008256)

LOCUS       V008256                 7174 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008256
VERSION     V008256
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7174)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7174)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7174)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7174)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(52..640)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(814..1671)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1672..1776)
                     /label="AmpR promoter"
     misc_feature    1878..2322
                     /note="5' region of EAF7 genes of Candida albicans; 5' of
                     EAF7"
     regulatory      2338..3810
                     /note="promoter region of ADH1 gene of Candida albicans;
                     CaADH1p"
                     /regulatory_class="promoter"
     regulatory      3824..4130
                     /note="terminator region of ACT1 gene of Candida albicans;
                     ACT1 terminator"
                     /regulatory_class="terminator"
     protein_bind    4167..4200
                     /label="Cre recombinase binding site"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT); loxP"
     CDS             5097..6215
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     protein_bind    complement(6468..6501)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6590..7115
                     /note="3' region of EAF7 genes of Candida albicans; 3' of
                     EAF7"