LOCUS       V008256                 7174 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008256
VERSION     V008256
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7174)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7174)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7174)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7174)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(52..640)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(814..1671)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1672..1776)
                     /label="AmpR promoter"
     misc_feature    1878..2322
                     /note="5' region of EAF7 genes of Candida albicans; 5' of
                     EAF7"
     regulatory      2338..3810
                     /note="promoter region of ADH1 gene of Candida albicans;
                     CaADH1p"
                     /regulatory_class="promoter"
     regulatory      3824..4130
                     /note="terminator region of ACT1 gene of Candida albicans;
                     ACT1 terminator"
                     /regulatory_class="terminator"
     protein_bind    4167..4200
                     /label="Cre recombinase binding site"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT); loxP"
     CDS             5097..6215
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     protein_bind    complement(6468..6501)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6590..7115
                     /note="3' region of EAF7 genes of Candida albicans; 3' of
                     EAF7"
ORIGIN
        1 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
       61 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      121 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      181 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      241 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      301 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
      361 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
      421 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
      481 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
      541 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
      601 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
      661 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
      721 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
      781 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
      841 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
      901 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
      961 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     1021 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     1081 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     1141 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     1201 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     1261 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     1321 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     1381 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     1441 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     1501 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     1561 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     1621 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     1681 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     1741 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     1801 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     1861 gccaggtcga ctctagacat tggtccatcg agaatgagat aaagctattc agcttattgt
     1921 gcgattataa gcccgctggt aaaaacaaac aacacaatat taaaatcatt atcaacaata
     1981 ttaatgaatc tttagactct aaggaggaac ccttcagcga atatgacgtg tggaataaat
     2041 tgaggtcttt atatgatcta gaaaagattg accagattga ggaattactg actgagaaca
     2101 ccagtacaga agaaaccaca aaccaagtgc tgtctggatc tgcaaatatc acgaaggaca
     2161 ccaagaacgc taaaactact ttggatgcaa aagagccaat tcaagataag aaagaaatgg
     2221 ataaagagaa atcagaatcg tcatcattgt ctacccctga accaccacaa agaagaacta
     2281 gaagtgccag agatacaaca aaatcgaaac gtaacttgcg tgcgatccgt ctaggattga
     2341 acgatgatat taagcgagaa ttcacatttt tctagaggaa tcacagttac aattgtagag
     2401 gaaaccgaac tagtaaaact agcacccaac acttctaaac aaaccaatca attcaatggt
     2461 ataacaaatc gtctaccaga agttatgcta atatgaaaca tcaattgaca acatggacat
     2521 caactgaagc tttaacaaat gaataaaaaa aaaagcccat acaatagacc attggtcata
     2581 gtttcatttg cccccgcccc acttttattg ttgagttgaa taattgtgag catcagctga
     2641 ttttaaaatt ataaattttt tttttcgtcc attgttaatt ctattttagt tttttcttct
     2701 ccatcgtctc cgaattattc cggaagctgg tagcggataa gttttagaat cattgagcct
     2761 cttttcaatg ccagatgtta tctgcaactg atggttagtt agttagtata agacttaaat
     2821 tctcatatag gtcatgtgca cggacaagct cattgagtga cgaaaagtca aatttaaact
     2881 tctctaaaca aatttcagac aatagtccga aaaaaaaaaa caaaacccat tattgtcgac
     2941 atgtcaaagg attcaacggt tgagatggag ccgtcaaata tccaaaacac tcttctttca
     3001 actaatttgc cgtaaactat ctccatcgta cgattaagat aaaaaaaaac aattatttct
     3061 tacaatattt gatagagacc caatgcaaag ccagcgagaa agaggagtat tggcattgtt
     3121 gggtagtaga tgaatgagta atcttctagc caaaacaaaa taaaccacaa gaacagtagg
     3181 caaaactgag tacttgatag cgcttgtagt ctttgtattt aaaaaaacct aattgtccaa
     3241 aaattgaaaa tcatgagttg caaatttgtt gaaatcacca ggtaaataga tcatttagct
     3301 atatcgcctt atcattcttc gtacaagaat tatacacaaa taccaccacc acaccaccca
     3361 gaaattttac acgtgttttc taaattatta ctttccgtgg ccaatcaaat tgaaattttt
     3421 cttgttcgaa acggcagtgc ctcaaacatt atagtagcaa gtattgccta aagagtttcc
     3481 gtctcgtgcc ccccccctat ccccttttgt aatttgccaa aatagctcaa aaaggataat
     3541 cccgcacttt ttttttccga gttctcgtca tgcaagcaag cttattcaga attttcagag
     3601 gtgcgagtct caccactacc actgcagctg catcttttac agcaacagca acagcaggtg
     3661 ccaccacggc aaagacattg tctggatcca ctgtgctccg aaaactgtat aaaaggacct
     3721 attcatccct ggtcttatct tctccagaat tatttttttt tcatcagttt aacaacaaca
     3781 aacgttattg tcatacaaca acaacaacaa ccgtgcatcg ctaccattgt tcaccacaaa
     3841 tgtttctaag agtgaaattc tggaaatctg gaaatctggt tttgtattct tgttattctt
     3901 ctttttgtta ttacatatat aacttgttac ttttttaaaa aaatctttgt ttattttata
     3961 aatatataaa actaaattta agaaaaagag aaaaatgttt tatttgagag attgaaattt
     4021 tacttgaatt tagcttagct tttataaagt attattatgt aaaaaaacaa aacaaatata
     4081 cattaaaaag ttaagactat aaaatagcca cccaaggcat ttctatatct acagtctgcc
     4141 agacttgcca cctgacgtct aagaaaataa cttcgtatag catacattat acgaagttat
     4201 agctcggatc cactagtaac ggccgccagt gtgctggaat tcgcccttgg atccaatcat
     4261 cactggtggg ggtcaagaaa gaggtttgag atcaggtact ttggcaccac cattagtagc
     4321 tgggtttggt gaagctgcaa gattaatgaa acaagaatct gcatttgaca agaaacatat
     4381 tgagaggtta tctactaaat tgaagaacgg tttgttatcc attccatcta ctcagtttaa
     4441 cggttgtaat aacccaacat atcaataccc aggttgtgtg aatgtttcct ttgcatacat
     4501 tgaaggggaa tctttgttaa tggcattgaa agatattgca ttgagttctg gttctgcatg
     4561 tacctctgca tctttggaac catcgtatgt tttacatgct ttgggtgccg atgatgcctt
     4621 ggctcattct tccattagat tcggtattgg tagattcacc actgaagcag aagttgacta
     4681 tgttattcaa gcaattaatg aaagagttga tttcttgaga aagatgtctc cattatggga
     4741 aatggttcaa gaaggtattg acttgaactc tatcgaatgg agtggacatt aaggtttttt
     4801 gtttttttgt aatttgtgtt gttatgtgta attatcgtgt atagagtaat ttcggaaatt
     4861 gtactttcgt tcatgtatct gaaccagatg aataatacgt tcgtattgtt gaagaatgac
     4921 taactcctgt aacggcgtgc gtgggttccg cagccgggtt caattttttt tttcttgttc
     4981 actataaaaa tatataataa acgaaaaaaa tccgagggac tcaggctatg aaatttttca
     5041 ctcaagaatt ttttttcctt catccttaat tcctttattc actacccaaa cccacaatgt
     5101 cagttaaaac taaaactatt acaattttac caggtgatca cgtcggtacc gaaattgtca
     5161 atgaagctat caaagtttta gaagcaattg aagctgccac tccttaccaa aaaatccatt
     5221 tcgatttcaa acatcattta atcggtggtg ctgccattga tgccactggt gtcccattgc
     5281 cagatgacgc acttgaaagt gccaaaaaat ctgatgccgt cttattgggt gctgttggtg
     5341 gaccaaaatg ggggactggc gccgttcgtc cagaacaagg tttattaaag attcgtaaag
     5401 aattgaacct ttatgctaac atcagaccat gtaactttgc cagtgactcc ttattggaat
     5461 tatccccatt gagaccagaa gtcgttaaag gtacaaactt gatcattgtt cgtgaattag
     5521 ttggtggtat ttatttcggt gatcgtgaag aacaagaaga aagtgaagat aaacaaaccg
     5581 catgggatac tgaaaaatac accgtcgatg aagtcaccag aatcacccgt atggctgcct
     5641 tcatggcttt acaacataac ccaccattac caatctggtc attggataag gctaatgttt
     5701 tagcttcttc tagattatgg agaagaactg tcgataaagt gatttctgaa gagttcccaa
     5761 ctttgtctgt tcaacatcaa ttgattgact ccgccgccat gattttaatt caaaacccaa
     5821 ccaaattgaa tggtataatc atcacttcta acatgtttgg tgatatcatt tccgatgaag
     5881 cttcagttat tccaggttcc ttgggtttat taccatctgc atctttggct tcattgccag
     5941 ataccaacac cgcttttggt ctttatgaac catgtcacgg ttctgctcct gatttaccag
     6001 ccaataaagt taacccaatt gctactattt tatctgctgc ttctatgttg agattatctt
     6061 tggattgtgt gaaagaagct gaagctttag aagaagccgt gaaacaagtc ttggataaag
     6121 gtatcagaac tgcagattta agaggtacca gttcagctac tgaagttggt gacgcgattg
     6181 tcgaagctgt tactaaaatc ttaaaagaaa aagcttaagt aaagtataca caaaatttaa
     6241 cgaatattat taatacaaaa tgaacaccac ctaaatactt ttttttaaaa atgtaccgac
     6301 tcttctaaaa attgcgataa ttcaatatcg gaaatttctc tcaccggttt acttggatct
     6361 tcggaaataa atctatttga agtaatggta gcctcacttg attctgattg gctctttcta
     6421 gacatgggta ggtaagggcg aattctgcag atatccatca cactggtata acttcgtata
     6481 gcatacatta tacgaagtta tggtaatacg gttatccaca gaatcagggg ataacgcagg
     6541 aaagaacatg tgagcaaaag gcctaactag atagcagtca gtcagtcagc aaagataagg
     6601 gggatgagac aattgctaaa attaagaaag aaaacacttc tgagcaagaa gaagataaaa
     6661 acaaaaagca gacagaagaa gaggcagaag aagaggaaga agaaggtgat catgttagtg
     6721 gagatgaaag ggaaagcaaa aataaggaca ctagtgaaga tgaggatggt gaagataatg
     6781 aagatcaaga tgaagatgat gttgaagtaa ttgaggacag ccatactgat aaagaaaatg
     6841 aatctagttc agaagaatca tcacctgaac caatgactaa acgaacgaga caaagaacaa
     6901 ggtccaccca tgaaaaaaca gaacacacgt cacctaatat caaaaagaga acaagacaga
     6961 gtgtcaaatt tgacgacaag cctgcagagc cctccgatac aggttctaga aaaagacgag
     7021 ctccgccagg atcagtatct cctccacatc ctcctaaaac acgaagacgt acaagatcag
     7081 taacccatga aatagaggaa gaggatgctt cgacagtaat catggtcata ccgcggaggc
     7141 ctgggcccga taacgcagga aagaacatgt gagc
//
