pCPC20 vector (V008257)

LOCUS       40924_12960        8158 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCPC20, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient 
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8158)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 8158)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8158)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-JAN-2018) Research Center of Bioenergy "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(179..767)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(941..1798)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1799..1903)
                     /label=AmpR promoter
     misc_feature    2005..2400
                     /note="5' region of ADE2 genes of Candida albicans; 5' of
                     ADE2"
     regulatory      2416..3888
                     /note="promoter region of ADH1 gene of Candida albicans; 
                     CaADH1p"
                     /regulatory_class="promoter"
     regulatory      3902..4208
                     /note="terminator region of ACT1 gene of Candida albicans;
                     ACT1 terminator"
                     /regulatory_class="terminator"
     protein_bind    4245..4278
                     /label=Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT); loxP"
     CDS             5360..6754
                     /codon_start=1
                     /product="Arg4"
                     /label=Arg4
                     /protein_id="AVR52627.1"
                     /translation="MSQQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADLTGTK
                     VYTQGLNKLGLITTEELNLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGEIIGK
                     NISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMPGYTH
                     LQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREFLAKG
                     LGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLADAYS
                     TGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLFDALT
                     TVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISGECVR
                     KAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDAIGGTAKSAVLKQLEN
                     LKSILN"
     protein_bind    complement(7572..7605)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."