LOCUS       40924_12960        8158 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCPC20, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient 
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8158)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 8158)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8158)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-JAN-2018) Research Center of Bioenergy "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(179..767)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(941..1798)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1799..1903)
                     /label=AmpR promoter
     misc_feature    2005..2400
                     /note="5' region of ADE2 genes of Candida albicans; 5' of
                     ADE2"
     regulatory      2416..3888
                     /note="promoter region of ADH1 gene of Candida albicans; 
                     CaADH1p"
                     /regulatory_class="promoter"
     regulatory      3902..4208
                     /note="terminator region of ACT1 gene of Candida albicans;
                     ACT1 terminator"
                     /regulatory_class="terminator"
     protein_bind    4245..4278
                     /label=Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT); loxP"
     CDS             5360..6754
                     /codon_start=1
                     /product="Arg4"
                     /label=Arg4
                     /protein_id="AVR52627.1"
                     /translation="MSQQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADLTGTK
                     VYTQGLNKLGLITTEELNLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGEIIGK
                     NISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMPGYTH
                     LQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREFLAKG
                     LGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLADAYS
                     TGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLFDALT
                     TVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISGECVR
                     KAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDAIGGTAKSAVLKQLEN
                     LKSILN"
     protein_bind    complement(7572..7605)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gcaccaaagc gtggcttgaa atgtatcatt gctggtgcag gtggtgctgc tcatttgcca
       61 ggtatggtgt aatcatggtc ataccgcgga ggcctgggcc cgataacgca ggaaagaaca
      121 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      181 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      241 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      301 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      361 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      421 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      481 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      541 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      601 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
      661 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
      721 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
      781 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
      841 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
      901 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
      961 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     1021 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     1081 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     1141 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     1201 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     1261 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     1321 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     1381 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     1441 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     1501 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     1561 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     1621 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     1681 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     1741 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     1801 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     1861 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     1921 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     1981 tcacgaggcc aggtcgactc tagaaggtgg ccaattaggt cgtatgattg ttgaagcagc
     2041 acatagattg aatatcaaga ccgtaatttt ggacgcagcc aaatcaccag ctaaacagat
     2101 caatgctcta gacgatcacg ttgatggatc gttcacaaac tatgattcga ttgtgaaatt
     2161 agctgaaaag gcagacgtat taactgttga aatcgaacat gttgatgttg atgctttaat
     2221 caaagttcaa gagaaattcc ctaaagttga aatatatcca ttacctgaaa ccataagatt
     2281 gattcaagat aaatatttac agaaaaatca tttaatcaaa cacgatgttg cagttacaga
     2341 atctgttgca gttgaaacca acacagtcga tgacttgtta cacattgggg aaaagtttgg
     2401 cgatccgtct aggattgaac gatgatatta agcgagaatt cacatttttc tagaggaatc
     2461 acagttacaa ttgtagagga aaccgaacta gtaaaactag cacccaacac ttctaaacaa
     2521 accaatcaat tcaatggtat aacaaatcgt ctaccagaag ttatgctaat atgaaacatc
     2581 aattgacaac atggacatca actgaagctt taacaaatga ataaaaaaaa aagcccatac
     2641 aatagaccat tggtcatagt ttcatttgcc cccgccccac ttttattgtt gagttgaata
     2701 attgtgagca tcagctgatt ttaaaattat aaattttttt tttcgtccat tgttaattct
     2761 attttagttt tttcttctcc atcgtctccg aattattccg gaagctggta gcggataagt
     2821 tttagaatca ttgagcctct tttcaatgcc agatgttatc tgcaactgat ggttagttag
     2881 ttagtataag acttaaattc tcatataggt catgtgcacg gacaagctca ttgagtgacg
     2941 aaaagtcaaa tttaaacttc tctaaacaaa tttcagacaa tagtccgaaa aaaaaaaaca
     3001 aaacccatta ttgtcgacat gtcaaaggat tcaacggttg agatggagcc gtcaaatatc
     3061 caaaacactc ttctttcaac taatttgccg taaactatct ccatcgtacg attaagataa
     3121 aaaaaaacaa ttatttctta caatatttga tagagaccca atgcaaagcc agcgagaaag
     3181 aggagtattg gcattgttgg gtagtagatg aatgagtaat cttctagcca aaacaaaata
     3241 aaccacaaga acagtaggca aaactgagta cttgatagcg cttgtagtct ttgtatttaa
     3301 aaaaacctaa ttgtccaaaa attgaaaatc atgagttgca aatttgttga aatcaccagg
     3361 taaatagatc atttagctat atcgccttat cattcttcgt acaagaatta tacacaaata
     3421 ccaccaccac accacccaga aattttacac gtgttttcta aattattact ttccgtggcc
     3481 aatcaaattg aaatttttct tgttcgaaac ggcagtgcct caaacattat agtagcaagt
     3541 attgcctaaa gagtttccgt ctcgtgcccc ccccctatcc ccttttgtaa tttgccaaaa
     3601 tagctcaaaa aggataatcc cgcacttttt ttttccgagt tctcgtcatg caagcaagct
     3661 tattcagaat tttcagaggt gcgagtctca ccactaccac tgcagctgca tcttttacag
     3721 caacagcaac agcaggtgcc accacggcaa agacattgtc tggatccact gtgctccgaa
     3781 aactgtataa aaggacctat tcatccctgg tcttatcttc tccagaatta tttttttttc
     3841 atcagtttaa caacaacaaa cgttattgtc atacaacaac aacaacaacc gtgcatcgct
     3901 accattgttc accacaaatg tttctaagag tgaaattctg gaaatctgga aatctggttt
     3961 tgtattcttg ttattcttct ttttgttatt acatatataa cttgttactt ttttaaaaaa
     4021 atctttgttt attttataaa tatataaaac taaatttaag aaaaagagaa aaatgtttta
     4081 tttgagagat tgaaatttta cttgaattta gcttagcttt tataaagtat tattatgtaa
     4141 aaaaacaaaa caaatataca ttaaaaagtt aagactataa aatagccacc caaggcattt
     4201 ctatatctac agtctgccag acttgccacc tgacgtctaa gaaaataact tcgtatagca
     4261 tacattatac gaagttatag ctcggatcca ctagtaacgg ccgccagtgt gctggaattc
     4321 gcccttaaga acatttctgt accgcaaatg tatcgacttt ttcgatggtg tgttattgtt
     4381 gttttgttgc gaaatataat agcttctgct ttatatcctt tgtgttgatc cttcccctcc
     4441 tttcatactt gtaaaattac aattggtatt ttcaaccaat attaccacac gtcatgcact
     4501 aactcaaatg ggcaaaaaaa agagagatag gaattacgtc attttattat gtttttacgt
     4561 ggactagaag gttaagataa tttctggtta ttttgttgat aattgagttt tgttcgacct
     4621 ccaccaatct ttaaactctc gttgagtatt cattttaatg attttttgaa atcagaaggg
     4681 gtgtttctaa cccaggattg ttttattgag aggttgaaac atcaatgtgt tagtttattg
     4741 atttgggttg gtttaataga aaaatatcag taggtgttca ataattgtag tttattttct
     4801 tggctaaaat tgttgcgaaa aattgacaaa ataataacaa aaagtatttt agatgctaaa
     4861 acataatagt aggaatatct acctgaacca tttacattca acaactggca agaaccttcc
     4921 taataatttg caagagtaat cacaattaaa ccaaaatcaa agtttaccgt gtatatagtt
     4981 ggacagaaag ctgtcacatt ggtttaatac atagttgaat atgtatgcat gaaacaacaa
     5041 caatacaatt acagctcatg atccttcaat taatttcaac attcaaacaa cataacacct
     5101 tgtgtaaatg aggttatgag tacatgtgaa caacaaatct tatgactaaa ctcgggtcgt
     5161 gtacttttga cgattgagtc atttttacca attaggcaat taatttttgc taaattttca
     5221 ttaaccgcgc tggccatccc aacaaacaat ataaaagctg acaacgaaaa aaaaaaaaaa
     5281 aaaaaaaaaa ctccaattct gaaaaatttt tcttcttctt catactaaac aaaacaacta
     5341 taaagataat caattaataa tgtcacaaca acctagtgaa aataaattat ggggtggtcg
     5401 attcactggc gccactgatc cattaatgga tttatacaat gcttcattac cttatgataa
     5461 agttatgtat gatgcagatt taactggtac taaagtatat actcaaggat tgaacaaatt
     5521 aggattaatt accactgaag aattgaatct gattcatcaa ggtttagaac aaattagaca
     5581 agaatggcat gataataaat ttattattaa agctggtgat gaagatattc atactgctaa
     5641 tgaacgtaga ttaggagaaa tcattgggaa aaacatttct ggtaaagttc atactggtag
     5701 atccagaaat gatcaagttg ctactgatat gagaattttt gttcgtgaat cattacttaa
     5761 tttaagtaag attttacatc aattcattac agcaattctt gaacgagctc ataaagaaat
     5821 tgatgtatta atgcctggtt atactcattt acaaaaagct caacctatta gatgggctca
     5881 ttggttaagt tcatatgcaa cttatttcac tgaagattat aaacgtttac aagaaatcat
     5941 tactcgtgtt aatcaatctc cattaggatc tggtgcttta gctggtcatc cctacggtat
     6001 tgatcgtgaa tttttagcta aaggattagg atttgatggt gttattggga attctttaac
     6061 tgcagtttct gatcgtgatt ttgttgttga aagtttattt tggagtacat tatttatgaa
     6121 tcatatttct cgattttcag aagatttaat tatttattct agtggtgaat ttggatttat
     6181 aaagttagct gatgcttact ctactggttc atcattaatg ccacaaaaga aaaatcctga
     6241 ttcattagaa ttattacgtg ggaaaagtgg cagagtgttt ggtcagttat ctgggttttt
     6301 aatgtcaatt aaatccattc catcaaccta taataaagat atgcaagaag ataaagaacc
     6361 attatttgat gcattgacta ccgtagaaca ttcaatttta attgctactg gtgtgatttc
     6421 tactttactg attgataaac aaaatatgga aaaagcttta actatggata tgttagctac
     6481 tgatttagca gattatttag ttagaaaagg tgttccattt agagaaactc atcatatttc
     6541 tggtgaatgt gttagaaaag ctgaagaaga aaaattgtcg ggaattgacc aattatcatt
     6601 tgaacaattt caacaaattg attcacgttt tgaaaaagat gttatggaaa cttttgattt
     6661 tgaagctagt gtggaaagaa gagatgctat tggtggtact gctaagagcg ctgttttaaa
     6721 acagttagaa aatttgaaat caattttaaa ttaatttata aataatcata tagtattcaa
     6781 tgttttgttt tgtttttttt ttttttttta tggaaatgta caaacagtat ataatcaatt
     6841 aatagaaaga taccctgtat tccattgtat cgcctttggg atgtctataa taccaacttt
     6901 caagaatgag aaattaaaca acaatttctt tcaccaatca actcgtctat attcaataaa
     6961 ctcattctgg tcttttcaaa tatttaatgt tatcctgatc aaacttacaa atcaaaaatg
     7021 tatcaaaatc attcattagt tcttcttctt ttgttatctt tgtcactata tttctctcca
     7081 tccagaccaa aaatacttcc attgtgaaga tcgtctgaat cataatcagc aactacctct
     7141 tccatttcag agaattcaga tttttcaaaa taggtatcac gggccaagta cacacaagac
     7201 aaacgatacg agtgctggga tatttctttc aatgtatcac taaattcgtt gagatttaaa
     7261 gagagccctt tgactgcttt cattactttc ctcacatcat gagcatccac attcaaattt
     7321 cctgtagccc tttattctta gttttctttt cttattcttt tcttcgtaca ttgaaatcac
     7381 aaatggtgat atcaaacgaa ggtcacactg acttatgtct aatacctcca atgatgatac
     7441 tggtaactta accaggtcta ctcttttcaa acgatttctc attagtgtca atttacgtag
     7501 gttcttcaac ctttcaaacg atgcaaatag cttttgtaag ggcgaattct gcagatatcc
     7561 atcacactgg tataacttcg tatagcatac attatacgaa gttatggtaa tacggttatc
     7621 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggcctaa ctagatagca
     7681 gtcagtcagt caggctcatg ttcgtgctgt cacaggatta cctatgccaa aggggttcac
     7741 tgaattctcc acttccatca ccaatgctat tatgttgaat gttttgggtg ataaggcaac
     7801 tccaaataaa gaattggaga tttgtcgccg tgctttggaa actccacatg cgtcagtgta
     7861 tctttatggt aaaactacta gaccagagcg aaagatgggt catatcaatg ttgtcacatc
     7921 ttccatgcaa gatgccgaga gtagattaag ttatatattg ggtgatacca ctgaaattcc
     7981 taaatcttta gctaccgaca aggaatctcc attggtggga ataataatgg gttccgattc
     8041 ggatctacca gttatggcag ttggtgctcg tattttgaaa caatttggtg tgccatttga
     8101 actcacaatc gtaagtgcac acagaactcc tcatagaatg tctgagtatg ctattgaa
//
